Distribution of human fecal marker genes and their association with pathogenic viruses in untreated wastewater determined using quantitative PCR

Ahmed, Warish; Bivins, Aaron; Payyappat, Sudhi; Cassidy, Michele; Harrison, Nathan; Besley, C.H.

doi:10.1016/j.watres.2022.119093

Cited by 19 publications

(7 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study, 36 untreated sewage samples were collected over a period of 12 months from two different wastewater treatment plants (WWTPs) in Australia, and average log 10 -transformed HF183/PMMoV ratios were approximately 0.5 at both plants. Analysis of the full data set from this study (kindly provided by Warish Ahmed) showed minimal temporal fluctuations in the HF183/PMMoV ratio at WWTP A (despite slight dips in the concentrations of HF183 and PMMoV), while the log 10 -transformed HF183/PMMoV ratio in WWTP B dropped below zero for 8 out of 10 consecutive samples, with the lowest value reaching −1.47.…”

Section: Resultsmentioning

confidence: 99%

“…Also, in untreated wastewater samples from six different systems in the Bay Area of California, USA, geometric mean concentrations of HF183 and PMMoV were 1.93 × 10 10 and 1.08 × 10 8 gc/L, respectively, which equates to a log 10transformed HF183/PMMoV ratio of 2.3 (although they measured HF183 rRNA instead of the HF183 rRNA gene). 29 In another study, 54 36 untreated sewage samples were collected over a period of 12 months from two different wastewater treatment plants (WWTPs) in Australia, and average log 10 -transformed HF183/PMMoV ratios were approximately 0.5 at both plants. Analysis of the full data set from this study (kindly provided by Warish Ahmed) showed Still, the log 10 ratios were greater than zero in most of the samples (83 and 78% of the samples from WWTPs A and B, respectively).…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ratio of HF183 to PMMoV in Sewage and Its Change When Exposed to Different Temperatures and Sunlight Conditions

Fani,

Rocha,

Howick

et al. 2024

ACS EST Water

View full text Add to dashboard Cite

In surface waters with fecal pollution, the age of fecal pollution has a direct connection to human health risk. Current microbial source tracking methods distinguish human-associated fecal markers from animal-associated biomarkers. However, in waters containing human-associated fecal pollution, current source tracking methods do not effectively distinguish small amounts of fresh (new) fecal pollution from large amounts of aged (old) fecal pollution. We introduced a concept for a model that considers environmental factors such as sunlight and temperature to estimate human fecal pollution age based on the ratio of two human-associated fecal biomarkers with different levels of persistence in the environment: HF183 and pepper mild mottle virus (PMMoV). We assessed the HF183/PMMoV ratio using (RT-)qPCR in 98 wastewater samples from two university residence halls in southern California, finding that HF183 was 15 times higher than PMMoV on average. This ratio decreased under sunlight and at higher temperatures due to differing decay rates of the two biomarkers. We propose that monitoring the concentration of HF183 and PMMoV, as well as the HF183/PMMoV ratio, can help to distinguish between a small amount of fresh contamination and a large amount of aged contamination.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Ratio of HF183 to PMMoV in Sewage and Its Change When Exposed to Different Temperatures and Sunlight Conditions

Fani,

Rocha,

Howick

et al. 2024

ACS EST Water

View full text Add to dashboard Cite

show abstract

“…The diversity in community function affects the composition of sewage. The concentration of human fecal marker genes (including crAssphage and PMMoV) were quite stable with little variation observed within and between WWTPs [ 54 ]. Thus, although some researchers detected the concentration of PMMoV, they did not normalize the concentration of SARS-CoV-2 genes, because PMMoV concentrations were similar in all samples, indicating that the composition (dilution degree) of sewage samples was stable [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Exploration on wastewater-based epidemiology of SARS-CoV-2: Mimic relative quantification with endogenous biomarkers as internal reference

Tang

et al. 2023

Heliyon

View full text Add to dashboard Cite

“…Samples from each event were collected in triplicate, 5 min apart. Dry-weather influent concentrations of human-associated MST marker gene concentrations were also measured from triplicate samples collected at influent sample points of two wastewater treatment plants in the Sydney region on 12 occasions from September 2019 to late September 2020 in a study by Ahmed et al ( 2022 ). They investigated the distribution of wastewater-associated marker genes and their association with pathogenic viruses in untreated wastewater.…”

Section: Methodsmentioning

confidence: 99%

Tracking contaminants of concern in wet-weather sanitary sewer overflows

Besley

Batley

Cassidy

2023

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

Four representative sites in the greater city of Sydney, Australia, were selected for a study of the wet-weather overflow of sanitary (separate to stormwater) sewerage systems. Water samples were collected by autosamplers from up to eight wet weather overflow events over 16 months and from companion receiving water sites. The objective was to identify the risks posed by sewage contaminants to aquatic biota in the receiving waters, to aid in prioritising management actions. Twelve organic contaminants were identified in influents across the four sites under rainfall ingress diluted conditions, with measurements showing that the highest concentrations were restricted to the anti-inflammatory acetaminophen and the diabetes medication metformin. Lesser contaminants included theobromine, ibuprofen, sucralose, and three benzotriazoles (mainly 1-H benzotriazole). An assessment of the toxicity of the identified organic chemicals indicated that none appeared to pose concerns for ecosystem health before wet-weather ingress dilution, and this was even less likely after dilution in the receiving waters. Metal concentrations were low; however, ammonia concentrations in the influent did pose a risk to ecosystem health, although receiving water dilution diminished this risk at four of the five receiving water locations studied.

show abstract

Distribution of human fecal marker genes and their association with pathogenic viruses in untreated wastewater determined using quantitative PCR

Cited by 19 publications

References 71 publications

Ratio of HF183 to PMMoV in Sewage and Its Change When Exposed to Different Temperatures and Sunlight Conditions

Ratio of HF183 to PMMoV in Sewage and Its Change When Exposed to Different Temperatures and Sunlight Conditions

Exploration on wastewater-based epidemiology of SARS-CoV-2: Mimic relative quantification with endogenous biomarkers as internal reference

Tracking contaminants of concern in wet-weather sanitary sewer overflows

Contact Info

Product

Resources

About