Distribution of adeG, adeB, adeE, adeY, abeM, and adeJ Efflux Pump Genes in Clinical Isolates of Acinetobacter Species from Korea

Choi, In-Sun; Choi, Ji Ae; Jang, Sook Jin; Park, Geon; Jeong, Seok Hoon; Kim, Choon-Mee; Lee, O-Jin; Kang, Seong‐Ho; Moon, Dae Soo

doi:10.3343/lmo.2019.9.4.201

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…In 2019, the prevalence of efflux pump genes was examined among 154 isolates of A. baumannii in Korea. The frequency of adeG, adeB, adeE, adeY, abeM and adej genes were 98.7%, 89%, 13.6%, 7.7%, 95.5% and 98.9% respectively (Choi et al 2019). In a similar investigation, the frequency of adeA, adeB, adeC, AbeM, and adeS genes in isolates of multidrug-resistant A. baumannii isolated from patients admitted to Alzahra Hospital in Isfahan was evaluated as 100%, 96%, 95%, and 8, respectively.…”

Section: Discussionmentioning

confidence: 93%

The frequency of efflux pump genes expression in Acinetobacter baumannii isolates from pulmonary secretions

et al. 2022

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Acinetobacter baumannii is an important opportunistic pathogen, and the cause of nosocomial infections worldwide in recent decades. Efflux pumps are considered as the important causes of multidrug resistance of A. baumannii. The aim of this study was to determine the frequency of efflux pump genes, and evaluate the antibiotic effect of Tigecycline on the expression of adeB gene in isolates of multidrug-resistant. A. baumannii. 70 isolates of A. baumannii were collected and confirmed by biochemical and molecular tests. Antibiotic resistance (Ciprofloxacin, Trimethoprim-sulfamethoxazole, and Tigecycline) was performed based on the minimum inhibitory concentration (MIC) method. Then, the effect of Carbonyl cyanide m-chlorophenyl hydrazone inhibitor (CCCP) on isolates was investigated and the frequency of adeB, adeG, adeJ and abeM genes were examined by PCR for isolates with reduced in MIC titer. Also, the antibiotic effect of Tigecycline on adeB gene expression in A. baumannii isolates was analyzed by Real-Time PCR. The antibiotic resistance for Ciprofloxacin, Trimethoprim-sulfamethoxazole, and Tigecycline was 97.1%, 95.8% and 37.2%, respectively. Following CCCP inhibitor use, the MIC titer had a decrease in MIC titer containing CCCP inhibitor was 64.3% for Ciprofloxacin, 51.5% for Trimethoprim-sulfamethoxazole and 50% for Tigecycline. The frequencies of genes associated with adeB, adeG, adeJ and abeM efflux pump were 100%, 92.8%, 86% and 98.5%, respectively. Real-Time PCR results showed a correlation between the antibiotic effects of Tigecycline on adeB gene expression. The antibiotic resistance of the isolates was relatively high. The isolates were resistant to Ciprofloxacin and Trimethoprim-sulfamethoxazole antibiotics, while more sensitive to Tigecycline. Also, efflux pump genes, which are the antibiotic resistance factors of A. baumannii, are frequently high in the isolates but it seems that isolates use other effluxe pumps than RND family to exit tigecycline. Graphical Abstract

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Section: Discussionmentioning

confidence: 93%

The frequency of efflux pump genes expression in Acinetobacter baumannii isolates from pulmonary secretions

et al. 2022

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“…Among the three structures, the most important transporter is a pump that cooperates with the outer membrane channel and the periplasmic adaptor protein (PAP) to promote the active efflux of many antibiotics and chemotherapeutic agents [5,6] . Therefore, the presence of efflux pumps in samples has been investigated by measuring this transporter even when measuring the presence or expression of various efflux pump genes [7,8] .…”

Section: Introductionmentioning

confidence: 99%

Role of Efflux Pump Gene adeIJK to Multidrug Resistance in Acinetobacter baumannii Clinical Isolates

Ji Ae Choi,

Choon-Mee Kim,

Sook-Jin Jang

et al. 2023

apm

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Background: The emergence of multidrug-resistant Acinetobacter baumannii (MDRAB) as a nosocomial pathogen is one of the major public health problems. This study aimed to evaluate the role of an efflux pump gene adeJ for the multidrug resistance of A. baumannii clinical isolates. Methods: Two groups, MDRAB and susceptible A. baumannii (SAB), of baumannii clinical isolates were studied. The SAB group consisted of strains that did not meet the criteria of MDRAB and were susceptible to more categories of antibiotics than MDRAB. Antimicrobial susceptibility results obtained by the VITEKⅡ system were used in data analysis and bacterial group allocation. We performed real-time reverse transcription PCR to determine the relative expression of adeJ. We compared the relative expression of adeJ in comparison groups by considering two viewpoints: (1) MDRAB and SAB groups,and (2) susceptible and non-susceptible groups for each antibiotic used in this study.Results: The mean value of relative expression of adeJ of MDRAB and SAB groups was 1.4 and 0.92, respectively, and showed significant differences (P = 0.002). The mean values of relative expression of adeJ of susceptible and non-susceptible groups to the antibiotics cefepime, ceftazidime, ciprofloxacin, imipenem, meropenem, tigecycline, piperacillin/tazobactam, ticarcillin/clavulanic acid, trimethoprim/sulfamethoxazole, piperacillin, and gentamicin showed statistically significant differences (P < 0.05). Conclusion: The overexpression of adeIJK might contribute to the multi-drug resistance in A. baumannii clinical isolates. Further, the overexpression of adeIJK might be one of the factors contributing to the resistance to numerous antibiotics.

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“…Acinetobacter baumannii ( A. baumannii ), which is well known for its tenacious survival in the environment and its ability to confer antibiotic resistance to other coexisting bacteria, 1 2 3 and the antimicrobial resistance are crucial problems that have caused high morbidity and mortality in critically ill patients. 4 5 6 The Korean Nosocomial Infections Surveillance System showed that, since 2010, A. baumannii was the most relevant causative organism of ventilator-associated pneumonia (VAP), and its resistance to carbapenem increased from 53% in 2006 to 90% in 2013.…”

Section: Introductionmentioning

confidence: 99%

Controlling an Outbreak of Multidrug-resistant Acinetobacter baumannii in a Pediatric Intensive Care Unit: a Retrospective Analysis

et al. 2021

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Background Multidrug-resistant Acinetobacter baumannii (MDRAB) is widespread among intensive care units worldwide, posing a threat to patients and the health system. We describe the successful management of a MDRAB outbreak by implementing an infection-control strategy in a pediatric intensive care unit (PICU). Methods This retrospective study investigated the patients admitted to the PICU in periods 1 (8 months) and 2 (7 months), from the index MDRAB case to intervention implementation, and from intervention implementation to cessation of MDRAB spread. An infection-control strategy was designed following six concepts: 1) cohort isolation of colonized patients, 2) enforcement of hand hygiene, 3) universal contact precautions, 4) environmental management, 5) periodic surveillance culture study, and 6) monitoring and feedback. Results Of the 427 patients, 29 were confirmed to have MDRAB colonization, of which 18 had MDRAB infections. Overall incidence per 1,000 patient days decreased from 7.8 (period 1) to 5.8 (period 2). The MDRAB outbreak was declared terminated after the 6-month follow-up following period 2. MDRAB was detected on the computer keyboard and in condensed water inside the ventilator circuits. The rate of hand hygiene performance was the lowest in the three months before and after index case admission and increased from 84% (period 1) to 95% (period 2). Patients with higher severity, indicated by a higher Pediatric Risk of Mortality III score, were more likely to develop colonization ( P = 0.030), because they had invasive devices and required more contact with healthcare workers. MDRAB colonization contributed to an increase in the duration of mechanical ventilation and PICU stay ( P < 0.001), but did not affect mortality ( P = 0.273). Conclusion The MDRAB outbreak was successfully terminated by the implementation of a comprehensive infection-control strategy focused on the promotion of hand hygiene, universal contact precautions, and environmental management through multidisciplinary teamwork.

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Distribution of adeG, adeB, adeE, adeY, abeM, and adeJ Efflux Pump Genes in Clinical Isolates of Acinetobacter Species from Korea

Cited by 5 publications

References 22 publications

The frequency of efflux pump genes expression in Acinetobacter baumannii isolates from pulmonary secretions

The frequency of efflux pump genes expression in Acinetobacter baumannii isolates from pulmonary secretions

Role of Efflux Pump Gene adeIJK to Multidrug Resistance in Acinetobacter baumannii Clinical Isolates

Controlling an Outbreak of Multidrug-resistant Acinetobacter baumannii in a Pediatric Intensive Care Unit: a Retrospective Analysis

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