Distribution of<i>vacA</i>genotypes in<i>Helicobacter pylori</i>strains isolated from Brazilian adult patients with gastritis, duodenal ulcer or gastric carcinoma

Ashour, Abdussalam Ali Ramadan; Magalhães, Paula Prazeres; Mendes, Edilberto Nogueira; Collares, Guilherme Birchal; Gusmão, Valquı́ria Ribeiro de; Queiroz, Dulciene Maria Magalhães; Nogueira, Ana Margarida Miguel Ferreira; Rocha, Gifone A.; Oliveira, C. A.

doi:10.1111/j.1574-695x.2002.tb00588.x

Cited by 70 publications

(10 citation statements)

References 28 publications

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“…As reported by [42,43] iceA1 expression is associated with a higher activity of the gastric inflammation, a condition that increases the risk for developing ulcer disease and gastric carcinoma.…”

Section: Discussionmentioning

confidence: 75%

Prevalence of H. pylori strains harbouring cagA and iceA virulence genes in saudi patients with gastritis and peptic ulcer disease

Kadi¹,

Halawani²,

Abdelkader³

2014

Microbiol Discov

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Aim: The study is aimed to detect the presence of cagA, iceA1, and iceA2 virulence genes in H. pylori from gastric biopsies, and to deduce the correlation between these genotypes and the two clinical outcomes peptic ulcer disease (PUD), and gastritis. Materials and methods: Thirty three Saudi patients 15 males and 18 females, 20 to 90 years were assigned into two groups PUD and gastritis. Genomic DNAs were extracted from biopsy specimens and used to detect the presence of cagA, and iceA genes by PCR typing system. Fisher's and Phi coefficient association tests were used for statistical analysis. Results: Genotyping show that both cagA and iceA genes were amplified from 27 specimens (81.7%). The prevalence of cagA+ and cagA-genotypes or iceA+ and iceA-genotypes did not differ significantly between males and females (p=0.070). Within the PUD and gastritis groups, the percentages of specimens positive for cagA gene were 76.9 % and 85 %, while those positive for iceA+ were 92.3% and 75% respectively. All cagA+/iceA+ combined genotypes was statically correlated with peptic ulcer (77%). This correlation was not observed within H. pylori specimens typed from gastritis. Patients with either PUD or gastritis were most likely infected by several strains of H. pylori. Conclusion: Different strains of H. pylori have virulent genotypes evidenced by PCR-based genotyping from biopsy specimens at a reasonable cost and time. These virulence strains spread at Taif province, may result in sever clinical outcomes such as ulcers which may be developed to cancer, the situation which necessitates further studies.

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Section: Discussionmentioning

confidence: 75%

Prevalence of H. pylori strains harbouring cagA and iceA virulence genes in saudi patients with gastritis and peptic ulcer disease

Kadi¹,

Halawani²,

Abdelkader³

2014

Microbiol Discov

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“…Cytotoxin associated gene A (CagA) is the most important pathogenic factor of H. pylori and plays a crucial role in the occurrence of gastric cancer [13]. Earlier studies by other groups have reported that CagA-positive H. pylori strains were isolated from almost all patients with gastric cancer [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Correlation between Helicobacter pylori Infection and Gastric Atrophy Examined in the Sera of Mongolian People

Amgalanbaatar

Shimomura

Sugano

et al. 2019

Gastrointestinal Disorders

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Serum specimens obtained from 680 individuals were examined to measure the amounts of pepsinogens 1 and 2, anti-CagA antibodies, and anti-Helicobacter pylori antibodies. We examined sera obtained from 610 Mongolian individuals living in the capital city, Ulaanbaatar. Seventy serum specimens were collected from Japanese people who were health-screened: These were stored at the gastroenterology laboratory of Jichi Medical University. The sera of the Japanese people were used as a control specimen. Two enzyme-linked immunosorbent assay (ELISA) kits, an E-plate ELISA kit from Eiken Chemical Co., Ltd. (Tokyo, Japan), and a Biohit ELISA kit from Biohit Oyj (Helsinki, Finland), were used for the detection of anti-H. pylori IgG antibodies in the sera of the 610 Mongolian people. An ELISA kit EIA-4138 from DRG Instruments GmbH (Germany) was used for the detection of anti-CagA IgG antibodies in the serum specimens. Serum pepsinogens were detected by an ELISA kit from Biohit Oyj. Of the 610 serum specimens, 385 specimens tested positive for the detection of anti-H. pylori antibodies using the two ELISA kits, and 47 tested negative. For the detection of anti-H. pylori antibodies by the Biohit ELISA kit, 560 and 50 specimens were positive and negative, respectively. The ratio of serum pepsinogen 1/2 was statistically lower (p < 0.0001) in the H. pylori-positive (560 specimens) than in the H. pylori-negative (50 specimens) specimens. However, the levels of serum pepsinogen 1 had no statistical significance (p = 0.465) between the specimens of the H. pylori-positive and -negative specimens. The ratio of serum pepsinogen 1/2 was 6.74 ± 0.12 in the H. pylori-positive specimens, whereas the ratio of serum pepsinogen 1/2 was 12.69 ± 1.02 in the H. pylori-negative specimens. This study demonstrated the high prevalence of H. pylori infection in Mongolian people, including young generations, and the people infected with H. pylori possessed low pepsinogen 1/2 ratios, indicating atrophic gastritis. The serological examinations by the two ELISA kits did not consistently reflect the prevalence of H. pylori infection in Mongolian people.

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“…The vac A is a polymorphic gene with two main signal region genotypes s1 and s2, and two different alleles in the mid region of the gene named m1 and m2. Infection with strains possessing the s1m1 genotype has been associated with precancerous gastric hypochlorhydria [17] and gastric carcinoma [19]. …”

Section: Introductionmentioning

confidence: 99%

Higher frequency of cagA EPIYA-C Phosphorylation Sites in H. pylori strains from first-degree relatives of gastric cancer patients

et al. 2012

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BackgroundTo evaluate the prevalence of more virulent H. pylori genotypes in relatives of gastric cancer patients and in patients without family histories of gastric cancer.MethodsWe evaluated prospectively the prevalence of the infection by more virulent H. pylori strains in 60 relatives of gastric cancer patients comparing the results with those obtained from 49 patients without family histories of gastric cancer. H. pylori status was determined by the urease test, histology and presence of H. pylori ureA. The cytotoxin associated gene (cagA), the cagA-EPIYA and vacuolating cytotoxin gene (vacA) were typed by PCR and the cagA EPIYA typing was confirmed by sequencing.ResultsThe gastric cancer relatives were significant and independently more frequently colonized by H. pylori strains with higher numbers of CagA-EPIYA-C segments (OR = 4.23, 95%CI = 1.53–11.69) and with the most virulent s1m1 vacA genotype (OR = 2.80, 95%CI = 1.04–7.51). Higher numbers of EPIYA-C segments were associated with increased gastric corpus inflammation, foveolar hyperplasia and atrophy. Infection by s1m1 vacA genotype was associated with increased antral and corpus gastritis.ConclusionsWe demonstrated that relatives of gastric cancer patients are more frequently colonized by the most virulent H. pylori cagA and vacA genotypes, which may contribute to increase the risk of gastric cancer.

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Distribution ofvacAgenotypes inHelicobacter pyloristrains isolated from Brazilian adult patients with gastritis, duodenal ulcer or gastric carcinoma

Cited by 70 publications

References 28 publications

Prevalence of H. pylori strains harbouring cagA and iceA virulence genes in saudi patients with gastritis and peptic ulcer disease

Prevalence of H. pylori strains harbouring cagA and iceA virulence genes in saudi patients with gastritis and peptic ulcer disease

Correlation between Helicobacter pylori Infection and Gastric Atrophy Examined in the Sera of Mongolian People

Higher frequency of cagA EPIYA-C Phosphorylation Sites in H. pylori strains from first-degree relatives of gastric cancer patients

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