Distribution of Ia-like molecules on the surface of normal and leukemic human cells.

Abstract

Antiserum to a glycoprotein antigen complex of 23,000 and 30,000 dalton subunits (p23,30), isolated and purified from a human lymphoblastoid B cell line, was shown to be highly specific for human bursal-equivalent-processed (B)

Immuno-histological diagnosis of lymphoproliferative diseases by selected combinations of antisera and monoclonal antibodies

“…Anti-Ia-like sera react strongly with B lymphocytes, normal myeloblasts, some monocytes, tissue macrophages and interdigitating reticular cells, and weakly with a small subpopulation of T lymphocytes (Schlossman et al, 1976;Winchester et al, 1977;Janossy et al, 1977Janossy et al, , 1980bFu et al, 1978). It is unreactive with thymocytes, most T lymphocytes, mature myeloid cells, platelets and 225 erythrocytes.…”

“…It is unreactive with thymocytes, most T lymphocytes, mature myeloid cells, platelets and 225 erythrocytes. In malignant-cell populations, anti-Ia-like serum reacts with cells of B lymphoproliferative diseases, non-T non-B ALL and some AML but fails to react with Thy-ALL, most T lymphomas, T-CLL and Sezary syndrome (Janossy et al, 1977;1980a;Schlossman et al, 1976;Winchester et al, 1977). C-anti-Ia serum was used at 1: 40 dilution on sections.…”

Ia-bearing T lymphocytes in man. Their identification and role in the generation of allogeneic helper activity.

“…Although previous studies from both this and other laboratories (1, 6) were not able to demonstrate Ia presence on human T lymphocytes, the ability to obtain highly purified T cells, optical improvement of immunofluorescence microscopy, and the high potency of the heterologous anti-Ia antiserum contributed to the identification of this small population. The existence of 2-6% Ia-positive lymphoeytes in a T-cell population which contained 99.5% sheep erythrocyte rosette-forming cells was detected.…”

“…Later, they were detected by hetero-antisera raised against the isolated protein from cell membranes (2)(3)(4). In addition to their presence on B cells and monocytes, they have been detected on the leukemic blast in eases of acute lymphocytic leukemia, acute myelogenous leukemia, and chronic myelogenous leukemia in blastic crisis (4)(5)(6). Recently, these Ia antigens were also shown to be present on the surface of precursor cells responsible for colony formation of both the myeloid monocytic and erythroid series (7)(8)(9) and on the surface of nonblood cells such as epidermal Langerhans cells (10).…”

Surface antigens in acute myeloblastic leukaemia: a study using heterologous antisera

“…TIUPCHONGx AND I. C. M. MACLENNAN monly associated with B-cells (Ia antigens) (Arbeit et al, 1975;Mann et al, 1975a) have been reported on the surface of a high proportion of acute leukaemia cells (Fu et al, 1975;Schlossmann et al, 1976;Billing et al, 1976Billing et al, , 1977. It is clear that several antisera which were previously reported to be specific for leukaemia are, in fact, directed against these antigens (Billing et al, 1976;Zighelboim et al, 1977).…”