Distribution of Immunoglobulins in Human Rectal Mucosa

Gelzayd, Eugene A.; Kraft, Sumner C.; Kirsner, Joseph B.

doi:10.1016/s0016-5085(68)80093-9

Cited by 42 publications

(8 citation statements)

References 22 publications

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“…The distribution of both SP and LF determined by the fluorescent studies agrees well with the immunological determination of these components in the corresponding fluids. The studies reported here are not consistent with those reporting intraepithelial cell ~,A staining in rabbit intestinal epithelium (19), parotid acini cells (20), and renal tubular epithelium (21), but are in agreement with those showing primarily an apical distribution in the human rectal mucosa and small intestine (3,4,22). Our findings are similar to those of Heremans and Crabb6 (22) and we are in general agreement with the hypothesis developed by these workers concerning the transport of ~A.…”

Section: Poscontrasting

confidence: 72%

The HUMAN SECRETORY IMMUNOGLOBULIN SYSTEM: IMMUNOHISTOLOGICAL LOCALIZATION OF ΓA, SECRETORY "PIECE," AND LACTOFERRIN IN NORMAL HUMAN TISSUES

Tourville

Adler

Bienenstock

et al. 1969

The Journal of Experimental Medicine

299

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Studies from a number of laboratories have suggested that a local immunological system supplies immunoglobulins to the fluids that bathe certain mucous membrane surfaces. The predominance of 5'A producing plasma cells in the lamina propria of the rectum and colon (1-5), stomach (6--8), duodenum, and jejunum (1, 8, 9) has been reported. Furthermore, 3,A-producing plasma cells have been shown to predominate in the bronchial (10-11) and nasal (7) mucosa as well as in the parotid (12) salivary gland.Immunofluorescent studies of human parofid salivary gland (12) showed that secretory "piece" was localized in acinar and ductular epithelial cells while "~A was restricted to plasma cells of the intersfifium, as well as the lumen of the glandular acini and ducts. On the basis of these results and other studies, a model was proposed for the synthesis of qcA and secretory "piece" (SP) in separate cells and a possible mechanism was suggested by which these components might combine to form the llS secretory "yA molecule (12).The present studies were undertaken to further investigate by means of immunofluorescent techniques, the cellular sites for 3'A and SP synthesis in a variety of normal human tissues. As a result of these investigations a revised model is presented involving at least in part, the intercellular (rather than intraceUular) transport of ~'A and its complexing with SP in extracellular (intercellular and luminal surface) locations. Moreover, the cellular sites of synthesis of SP and its relationship to epithelial mucins have been more clearly delineated by studies in a variety of epithelial tissues. Material and MethodsTissue Spevimens.--Immunohistochemlcal investigations were performed on various human tissue. 14 bronchial biopsies were obtained from two locations, eight from the carina and six

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Section: Poscontrasting

confidence: 72%

The HUMAN SECRETORY IMMUNOGLOBULIN SYSTEM: IMMUNOHISTOLOGICAL LOCALIZATION OF ΓA, SECRETORY "PIECE," AND LACTOFERRIN IN NORMAL HUMAN TISSUES

Tourville

Adler

Bienenstock

et al. 1969

The Journal of Experimental Medicine

299

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“…The same localization of Ig-positive cells in the lamina propria has been found in the respiratory, digestive, and female genital tracts (Brandtzaeg & Kraus, 1965;Crabbe and Heremans, 1966;Eidelman et al 1966;Brandtzaeg et al, 1967;Heremans & Crabbe, 1967;Gelzayd, Kraft & Fitch, 1967;Gelzayd, Kraft & Kirsner, 1968;Martinez-Tello, Braun & Blanc, 1968;Rossen et al, 1968;Soltoft, 1969;Tourville et al, 1969Tourville et al, , 1970Savilahti, 1972).…”

Section: Dtscussionmentioning

confidence: 59%

An immunohistopathological study on the synthesis of immunoglobulins and complement in normal and pathological skin and the adjacent mucous membranes

1974

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SUMMARY To visualize the cells in skin and mucous membranes in which synthesis of immunoglobulins and complement had been demonstrated, the direct and indirect immunofluorescence techniques were applied, using a fluorescence microscope with epi‐illumination, and blue narrow‐band excitation light. Ig‐positive cells were found in the lesional skin of patients with discoid lupus erythematosus, (bullous) pemphigoid, dermatitis herpctiformis, mycosis fungoides, atopic dermatitis, and lichen planus, and in the normal mucous membranes deriving from the conjunctiva, the nasal septum, the oral cavity and vagina, but not in the normal skin. The lesional skin showed mainly IgG‐positive cells, and the mucous membranes generally had IgG‐ and IgA‐positive cells. In the mucous membranes, with the exception of the oral mucosa, there was a predominance of IgA‐positive cells. The Ig‐positive cells were morphologically plasma cells and lymphocytes, and occurred not in groups but scattered in the dermis of the skin and in the lamina propria of the mucous membranes. These cells could be distinguished rather easily from eosinophilic granulocytes, which showed nonspecific fluorescence. The results of the immunofluorescence study were in good agreement with those obtained with the tissue culture technique. Complement‐positive cells could not be demonstrated with the polyvalent antiserum used. A possible explanation for this failure is discussed.

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“…Further support comes from the high incidence of chronic hepatitis in persons with hypogammaglobulinemia in whom delayed hypersensitivity is intact or even enhanced (106,107). Thus, while immu nologic mechanisms are active in the disease, their role in the progression of chronic hepatitis remains speculative (108). Nevertheless, immunologic considerations have served as a tremendous impetus for immunosuppres sive therapy which will be discussed later.…”

Section: Perpetuating Mechanismsmentioning

confidence: 99%