Distribution of insertion sequence IS200 in Salmonella and Shigella

Abstract

Two DNA probes for the detection of insertion sequence IS200 by either Southern blotting or colony hybridization were constructed. One of the probes is a 300 bp EcoRI-Hind111 fragment of IS200 cloned onto pBluescript KS( +); the other is a tail-to-tail dimer of the same fragment cloned onto pUC19. A survey of the presence of IS200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of Salmonella spp. examined contained one or more copies of insertion sequence IS200, with the exception of the subgenus I Serovar S. agona in which IS200 is not found. Although insertion sequence IS200 was first considered a Salmonellaspecific element, it also exists in many isolates of Shigella sonnei and Shigellajlexneri, but not in Shigella dysenteriae.

Interrelationships between strains ofSalmonella enteritidis

“…Like the strains of S. enteritidis PT 24 described above, these strains of S. enteritidis PT 23 were also found to possess an Inc N plasmid of 34 MDa which coded for resistance to ampicillin and streptomycin, but not to tetracyclines [6].…”

“…IS200 is a salmonella-specific insertion element first described by Lam and Roth [33] and distributed on conserved loci on the chromosome of many salmonella serotypes [34]. Certain characteristics of IS200 favour its use as a probe for strain discrimination and using IS200, three 'clonal lineages', designated SEClI, SEClII and SEClIII have been identified within the chromosomes of 27 of the S. enteritidis phage type strains [35].…”

Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in Microcystis

“…Significantly increased expression occurred most frequently for transposases from the family IS200/IS605, suggesting not only that nutrient limitation may regulate transposase gene expression, but that it specifically targets expression of a single family. This family of transposases is also widespread in the genomes of multiple pathogenic bacteria (Gibert et al, 1990): similar behavior of these elements among pathogens and proteomic identification of transposases in endosymbionts and an acid mine drainage community may have ramifications for the ability of such organisms to infect a host or adapt to an environment (Chao et al, 1983;Ram et al, 2005;Kleiner et al, 2013). Homologs of the IS200/605 family have been identified across both eukaryotes and eukaryote-infecting viruses, suggesting these results may have implications in all domains of life (Bao and Jurka, 2013).…”

Conventional and molecular approaches to isolates of Salmonella hadar from sporadic and epidemic cases

“…The enzyme used from the previously reported sporadic cases, allowing one to detect the peculiarity of the outbreak isolate. was PstI which does not cut within IS200 and provided the clear resolution (Gibert et al 1990). PstI analysis showed Each method used in this study was found to be efficient as an epidemiological marker; because of the rarity of isolation different profiles of IS200 insertion in Salm.…”

“…Agarose (Bio-Rad, Hercules, CA) for gel electrophoresis was used at Outbreak a concentration of 0·8%. Plasmid pIZ46 (kindly provided by Dr José Casadesùs, In September 1994 out of 2206 people, workers and waiters, having consumed lunch in a factory canteen in Montalto di University of Seville, Spain) containing a tail-to-tail dimer of the EcoRI-HindIII fragment of IS200 (Gibert et al 1990) was Castro (Viterbo), 437 developed gastroenteritis. Out of the Salmonella sp.…”