Distribution of Integron Iii and Phylogenic Clade Among MDR Uropathogenic E. Coli From Patient in Al-Diwaniyah City, Iraq

Abstract

The aim: The goal of this study is to identify Uropathogenic E. coli multidrug-resistant bacteria, as well as their genetic profile clade and correlation with dispersion integron. Materials and methods: Five hundred urine samples from UTI patients were collected in Al-Diwaniyah, Iraq. They were then investigated by a qualified consulting doctor. After cultivation in special media (MacConkey agar and Eosin-Methylene blue) to detect Enterobacteriacea, including Escherichia coli, the samples were identified using the Vitec 2 compact system, as well as MIC susceptibility testing to Amikacin, Levofloxacin, Cefepime, Meropenem, Nitrofurantion, and Trimethoprim-sulfamethoxazole for detect multidrug resistance isolates. Multiplex PCR was used to detect three types of integrase gene. Finally, ERIC2-PCR was used to detect the genetic profile of all isolates. Results: From 500 UTI samples, 22 isolates UPEC detected resistance to different class of antibiotic, including: 86.3% to Cefepime, 54.5% - to Trimethoprim-sulfamethoxazole, 31.8% -Levofloxacin, 18.8% - Amikacin, 18.8% - Imipenem, 0% to Nitrofurantion. Twenty of the 22 isolates had various integrase gene classes as: 54.5%, 36.3%, 72.2% for Intl I, Intl II, and Intl III, respectively; while two isolates have no integron. Conclusions: Integron III has a higher incidence and compensates other classes; isolates with triple classes are more virulent and antibiotic resistant. Their genomic profile reveals association with human urine and unique clade of relatives.

Detection and Characterization of hly,cdtI and stxI genes, in MDR uropathogenic and intestinal E.coli isolates, Hilla city, Iraq.

“…Despite various efforts, UTIs continue to impact 150 million people annually around the world, with significant morbidity and huge medical expenditures. A variety of virulence genes must be expressed in a coordinated manner for the UPEC mode of pathogenesis to create long-lasting infections [1] [2]. Escherichia coli expressing one or two different forms of Shiga toxin (Stx) are referred to as Shiga toxin-producing Escherichia coli (STEC) [3].…”