Distribution of Intrinsic Plasmid Replicase Genes and Their Association with Carbapenem-Hydrolyzing Class D β-Lactamase Genes in European Clinical Isolates of Acinetobacter baumannii

Towner, K. J.; Evans, Benjamin A.; Villa, Laura; Levi, K.; Hamouda, Ahmed; Amyes, Sebastian G. B.; Carattoli, Alessandra

doi:10.1128/aac.01661-10

Cited by 60 publications

(79 citation statements)

References 36 publications

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“…The largest group of strains belonged to GR6 (repAci6; 93 isolates), and the variations in rep gene content were even presented among epidemiologically related isolates. Further analysis of co-occurrence of rep genes and CHDL encoding genes revealed the association of bla OXA-58-like genes (22 strains) with repAci1, repAci3, repAci4, and repAciX genes, while bla OXA-40-like (6 strains) were related to repAci2 and p2ABSDF0001, and bla OXA-23-like (8 isolates) were correlated with repAci1 [93].…”

Section: Plasmidsmentioning

confidence: 99%

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Acinetobacter baumannii: biology and drug resistance — role of carbapenemases

Nowak

Paluchowska

2015

Folia Histochem Cytobiol.

107

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Acinetobacter baumannii is a Gram-negative, glucose-non-fermenting, oxidase-negative coccobacillus, most commonly associated with the hospital settings. The ability to survive in adverse environmental conditions as well as high level of natural and acquired antimicrobial resistance make A. baumannii one of the most important nosocomial pathogens. While carbapenems have long been considered as antimicrobials of last-resort, the rates of clinical A. baumannii strains resistant to these antibiotics are increasing worldwide. Carbapenem resistance among A. baumannii is conferred by coexisting mechanisms including: decrease in permeability of the outer membrane, efflux pumps, production of beta-lactamases, and modification of penicillin-binding proteins. The most prevalent mechanism of carbapenem resistance among A. baumannii is associated with carbapenem-hydrolysing enzymes that belong to Ambler class D and B beta-lactamases. In addition, there have also been reports of resistance mediated by selected Ambler class A carbapenemases among A. baumannii strains. Resistance determinants in A. baumannii are located on chromosome and plasmids, while acquisition of new mechanisms can be mediated by insertion sequences, integrons, transposons, and plasmids. Clinical relevance of carbapenem resistance among strains isolated from infected patients, carriers and hospital environment underlines the need for carbapenemase screening. Currently available methods vary in principle, accuracy and efficiency. The techniques that deserve particular attention belong to both easily accessible unsophisticated methods as well as advanced techniques based on mass spectrometry or molecular biology. While carbapenemases limit the therapeutic options in A. baumannii infections, studies concerning novel beta-lactamase inhibitors offer a new insight into effective therapy.

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Section: Plasmidsmentioning

confidence: 99%

“…Studies concerning the sequence analysis of A. baumannii plasmid replicons revealed the differences comparing to other prokaryotic species, suggesting that A. baumannii strains may carry distinct set of plasmid types [92,93]. It is believed that the most clinically relevant carbapenemases are often associated with plasmids [68].…”

Section: Plasmidsmentioning

confidence: 99%

Acinetobacter baumannii: biology and drug resistance — role of carbapenemases

Nowak

Paluchowska

2015

Folia Histochem Cytobiol.

107

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“…In this country, blaOXA-143 has been reported in frequencies ranging between 8.4% and 76% [29,46,47]. With regard to OXA-24 and OXA-58, although all Acinetobacter strains in this study were blaOXA-24 -and blaOXA-58 -, international literature suggests these oxacillinases are well spread among A. baumannii clinical isolates [13][14][15]28].…”

Section: Discussionmentioning

confidence: 81%

“…OXA-143 is still little studied and restricted to Brazil. It was identified in a clinical isolate of multidrug-resistant A. baumanni [26][27][28][29]. β-lactamase OXA-143 hydrolyzes penicillins and carbapenems but does not significantly hydrolyze expanded-spectrum cephalosporins, as observed with other carbapenem-hydrolyzing class D-β-lactamase [26].…”

Section: Introductionmentioning

confidence: 99%

Epidemiological characteristics and antimicrobial susceptibility among carbapenem-resistant non-fermenting bacteria in Brazil

Dias¹,

Diniz²,

Peter

et al. 2016

J Infect Dev Ctries

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Introduction: Non-fermenting Gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii are widespread in the environment and are increasingly associated with nosocomial infections. Extensive and indiscriminate use of antibiotics in hospitals has contributed to an increased number of infections caused by these microorganisms, that are resistant to a wide variety of antimicrobials, including β-lactams. This study aimed to isolate and identify carbapenem-resistant Acinetobacter spp. and P. aeruginosa from hospitalized patients, to determine their antimicrobial susceptibility patterns and to screen for blaOXA , and blaOXA-143 genes among the isolated bacteria. Methodology: Antimicrobial resistance patterns were performed using the disk-diffusion method. Genetic markers related to carbapenem resistance were screened by polymerase chain reaction. Results: Carbapenem-resistant Acinetobacter spp. (n = 44) and P. aeruginosa (n = 28) samples were isolated from patients admitted to a tertiary hospital. Polymyxin B was the only effective drug for all isolates. Considering the oxacillinase gene screening, genetic markers were observed only in Acinetobacter isolates. The most frequent genotype observed was blaOXA-23 + /blaOXA-51 + (45.5%), followed by blaOXA-51 + /blaOXA-143

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“…Although a number of different mechanisms of resistance to carbapenems have been reported, most clinically significant carbapenem resistance has been associated with plasmid mediated acquisition of genes encoding either class B metallo-β-lactamases or carbapenem hydrolyzing class D OXA type -β-lactamases, representing the most important and wide spread mechanism of carbapenem resistance [7]. This study is aimed to characterize the nature of plasmids among metallo-β-lactamase producing Pseudomonas aeruginosa isolates from the clinical samples and to detect the presence of bla VIM gene by PCR amplification procedure and to study the prevalence of the gene in our tertiary care hospital.…”

mentioning

confidence: 99%