Distribution of IS900 restriction fragment length polymorphism types among animal Mycobacterium avium subsp. paratuberculosis isolates from Argentina and Europe

Moreira, Ana Rita Silva; Paolicchi, Fernando Alberto; Morsella, Claudia; Zumárraga, Martín José; Cataldi, Angel; Bigi, Fabiana; Alito, A.; Piet, Overduin; Dick, van Soolingen; Isabel, Romano Marı́a

doi:10.1016/s0378-1135(99)00144-3

Cited by 37 publications

(28 citation statements)

References 11 publications

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“…The results obtained with the current panel of strains comprising 71 isolates from 14 German herds supplements these data with BstEII-based IS900 RFLP types C17, C5, C16, C18, C22, and C new , which are otherwise rare. Besides Europe, IS900 RFLP type C1 is highly prevalent in the United States (33,46) and New Zealand (9), whereas type C3 predominates in Australia (10,36,47) and type C17 predominates in specific regions of Argentina (25). As documented with our results for Germany, type C17 is rare in Europe and up to now has only occasionally been detected in isolates from Scotland (12, 18).…”

Section: Discussionsupporting

confidence: 78%

“…Therefore, the IS900 RFLP profiles differ depending on whether the hybridization site of the probe is to the 5Ј or 3Ј site of the digestion site of the restriction enzyme or covers the digestion site. In the latter case, the probe recognizes IS900 on both sides of the PstI site (25) (12); and corresponding patterns were shown after digestion with one or both of these enzymes. In contrast, IS900 contains no restriction site for BstEII or BamHI.…”

Section: Discussionmentioning

confidence: 95%

“…By using M. avium subsp. paratuberculosis isolates from different host species and different geographic regions of the world, a number of subtypes could be charac-terized, especially in the type II group of strains (9,10,25,27,32,33,46). Type II strains were mainly isolated from cattle; but they were also isolated from sheep, goats, different wildlife species, and humans.…”

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High Genetic Diversity among Mycobacterium avium subsp. paratuberculosis Strains from German Cattle Herds Shown by Combination of IS 900 Restriction Fragment Length Polymorphism Analysis and Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem-Repeat Typing

Möbius

Luyven²,

Hotzel

et al. 2008

J Clin Microbiol

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Mycobacterium avium subsp. paratuberculosis is the etiologic agent of Johne's disease and is endemic to the national cattle herds of many countries. Because of the very low level of genetic heterogeneity of this organism, it is difficult to select a workable procedure for strain differentiation at a resolution sufficient to investigate epidemiological links between herds or different ruminant species and the suggested zoonotic potential of M. avium subsp. paratuberculosis for Crohn's disease. Analysis of restriction fragment length polymorphisms (RFLPs) based on the insertion element IS900 (IS900 RFLP) with four restriction enzymes and 10 markers of specific mycobacterial interspersed repetitive units (MIRUs) and variable-number tandem repeats (VNTRs) was applied to 71 bovine M. avium subsp. paratuberculosis isolates originating from 14 herds from different regions in Germany. Among these isolates, all of which belonged to the M. avium subsp. paratuberculosis type II group, 17 genotypes were detected by IS900 RFLP and consisted of a combination of seven BstEII, eight PstI, nine PvuII, and four BamHI restriction patterns. Novel RFLP types were found. The diversity of the M. avium subsp. paratuberculosis isolates inside the herds was different depending on the frequency of animal purchase. The results of typing by IS900 RFLP and MIRU-VNTR analyses were not associated. Fifteen MIRU-VNTR patterns were identified with a discriminatory index of 0.905. The most common BstEII-based IS900 RFLP type, type C1 (72%), was subdivided into 14 types by MIRU-VNTR analysis. A combination of fingerprinting and PCR-based techniques resulted in 24 M. avium subsp. paratuberculosis genotypes and achieved a discriminatory index of 0.997. By using only BstEII and PstI digestion together with typing by MIRU-VNTR analysis, a discriminatory index of 0.993 was achieved. This is high enough to support epidemiological studies on a national as well as a global scale.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

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High Genetic Diversity among Mycobacterium avium subsp. paratuberculosis Strains from German Cattle Herds Shown by Combination of IS 900 Restriction Fragment Length Polymorphism Analysis and Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem-Repeat Typing

Möbius

Luyven²,

Hotzel

et al. 2008

J Clin Microbiol

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“…Of 33 cervine-derived M. avium subsp. paratuberculosis isolates described (20 from New Zealand [9], 11 from South America [26], and 2 from Denmark [31]), only 3 had been reported to exhibit RFLP banding patterns similar to those of ovine strains of M. avium subsp. paratuberculosis.…”

Section: Vol 74 2006 Immune Response To Johne's Disease In Deer 3533mentioning

confidence: 99%

Immunological and Molecular Characterization of Susceptibility in Relationship to Bacterial Strain Differences inMycobacterium aviumsubsp.paratuberculosisInfection in the Red Deer (Cervus elaphus)

O’Brien

Mackintosh²,

Bakker³

et al. 2006

Infect Immun

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Johne's disease (JD) infection, caused by Mycobacterium avium subsp. paratuberculosis, represents a major disease problem in farmed ruminants. Although JD has been well characterized in cattle and sheep, little is known of the infection dynamics or immunological response in deer. In this study, typing of M. avium subsp. paratuberculosis isolates from intestinal lymphatic tissues from 74 JD-infected animals showed that clinical isolates of M. avium subsp. paratuberculosis from New Zealand farmed red deer were exclusively of the bovine strain genotype. The susceptibility of deer to M. avium subsp. paratuberculosis was further investigated by experimental oral-route infection studies using defined isolates of virulent bovine and ovine M. avium subsp. paratuberculosis strains. Oral inoculation with high (10 9 CFU/animal) or medium (10 7 CFU/animal) doses of the bovine strain of M. avium subsp. paratuberculosis established 100% infection rates, compared to 69% infection following inoculation with a medium dose of the ovine strain. The high susceptibility of deer to the bovine strain of M. avium subsp. paratuberculosis was confirmed by a 50% infection rate following experimental inoculation with a low dose of bacteria (10 3 CFU/animal). This study is the first to report experimental M. avium subsp. paratuberculosis infection in red deer, and it outlines the strong infectivity of bovine-strain M. avium subsp. paratuberculosis isolates for cervines.

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“…These elements have also been used for typing culturable isolates of M. avium subsp. paratuberculosis (McFadden et al, 1987 ;Whipple et al, 1990 ;Moss et al, 1991 ;Pavlik et al, 1995Pavlik et al, , 1999Bauerfeind et al, 1996 ;Collins et al, 1997 ;Whittington et al, 1998 ;Moreira et al, 1999). This approach to the molecular typing of M. avium subsp.…”

Section: Abbreviation : Mpil Multiplex Pcr Of Is900 Locimentioning

confidence: 99%

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

et al. 2000

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Mycobacterium avium subsp. paratuberculosis is a pathogen that causes chronic inflammation of the intestine in many animals, including primates, and is implicated in Crohn's disease in humans. It differs from other members of the M. avium complex in having 14-18 copies of IS900 inserted into conserved loci in its genome. In the present study, genomic DNA flanking 14 of these insertions was characterized and homologues in the Mycobacterium tuberculosis and M. avium subsp. avium genomes were identified. These included regions encoding a sigma factor (sigJ) at locus 3, a nitrate reductase (nirA) at locus 4, a transcription regulator (tetR) and polyketide synthase at locus 6, and a 6-O-methylguanine methyltransferase at locus 9. In addition, locus numbers were assigned to 9 of 15 RFLP bands previously described. IS900 insertion at 7 of the 14 characterized loci was into the RBS of a gene substituting an RBS encoded by IS900 sited two bases closer to the initiation codon. IS900 insertion at five loci interrupted an ORF at the target site, one of which encoded a homologue of the immunodominant mycobacterial DesA1 protein. Eleven of eighty-one M. avium subsp. paratuberculosis isolates lacked the insertion site at locus 6 together with flanking genomic DNA. This region was also absent from seven reference strains of M. avium subsp. avium, from one M. avium subsp. silvaticum and from six other mycobacterial species. A multiplex PCR of IS900 loci (MPIL) typing method was developed which was able to discriminate 10 different types of M. avium subsp. paratuberculosis from the panel of 81 isolates with consistent differences between those of bovine and ovine origin. Nine MPIL types corresponded with a single PstI/BstEII RFLP type, suggesting that this method may be applicable to typing of M. avium subsp. paratuberculosis directly from a sample without the need for culture. The remaining MPIL type corresponded with seven PstI/BstEII RFLP types. Further resolution of these may come from sequencing the remaining four uncharacterized IS900 loci.

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Distribution of IS900 restriction fragment length polymorphism types among animal Mycobacterium avium subsp. paratuberculosis isolates from Argentina and Europe

Cited by 37 publications

References 11 publications

High Genetic Diversity among Mycobacterium avium subsp. paratuberculosis Strains from German Cattle Herds Shown by Combination of IS 900 Restriction Fragment Length Polymorphism Analysis and Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem-Repeat Typing

High Genetic Diversity among Mycobacterium avium subsp. paratuberculosis Strains from German Cattle Herds Shown by Combination of IS 900 Restriction Fragment Length Polymorphism Analysis and Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem-Repeat Typing

Immunological and Molecular Characterization of Susceptibility in Relationship to Bacterial Strain Differences inMycobacterium aviumsubsp.paratuberculosisInfection in the Red Deer (Cervus elaphus)

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

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