Distribution of Karlodinium veneficum in the coastal region of Xiangshan Bay in the East China Sea, as detected by a real-time quantitative PCR assay of ribosomal ITS sequence

Huang, Hailong; Shao, Qianwen; Zhu, Xiaojuan; Luo, Jie; Meng, Ran; Zhou, Chengxu; Zhu, Peng; Zhu, Yan; Yan, Xiaojun

doi:10.1016/j.hal.2018.12.001

Cited by 21 publications

(7 citation statements)

References 38 publications

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“…However, this method seems inadequate in areas where cells are not abundant enough to be easily detected. Molecular methods based on the analysis of environmental DNA (eDNA) are now used to detect the presence of rare species of microorganisms, especially for the conservation and monitoring of endangered species (Jerde et al ., 2011; Taberlet et al ., 2012; Thomsen and Willerslev, 2015; Barnes and Turner, 2016) but also for monitoring harmful algal species (Galluzzi et al ., 2004; Fitzpatrick et al ., 2010; Garvetto et al ., 2018; Huang et al ., 2019). Standard and quantitative real‐time PCR or quantitative (rt‐PCR or qPCR) methods, based on the analysis of species‐specific Internal Transcribed Spacer (ITS)‐5.8S and Large Sub Unit (LSU) ribosomal DNA, have been developed and applied to the monitoring of Ostreopsis species (Penna et al ., 2007; Battocchi et al ., 2010; Casabianca et al ., 2013, 2014).…”

Section: Introductionmentioning

confidence: 99%

Current distribution and potential expansion of the harmful benthic dinoflagellateOstreopsiscf.siamensistowards the warming waters of the Bay of Biscay,North‐EastAtlantic

Drouet

Jauzein

Herviot-Heath

et al. 2021

Environmental Microbiology

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In a future scenario of increasing temperatures in North-Atlantic waters, the risk associated with the expansion of the harmful, benthic dinoflagellate Ostreopsis cf. siamensis has to be evaluated and monitored. Microscopy observations and spatiotemporal surveys of environmental DNA (eDNA) were associated with Lagrangian particle dispersal simulations to: (i) establish the current colonization of the species in the Bay of Biscay, (ii) assess the spatial connectivity among sampling zones that explain this distribution, and (iii) identify the sentinel zones to monitor future expansion. Throughout a sampling campaign carried out in August to September 2018, microscope analysis showed that the species develops in the south-east of the bay where optimal temperatures foster blooms. Quantitative PCR analyses revealed its presence across almost the whole bay to the western English Channel. An eDNA timeseries collected on plastic samplers showed that the species occurs in the bay from April to September. Due to the water circulation, colonization of the whole bay from the southern blooming zones is explained by inter-site connectivity. Key areas in the middle of the bay permit continuous dispersal connectivity towards the north. These key areas are proposed as sentinel zones to monitor O. cf. siamensis invasions towards the presumably warming water of the North-East Atlantic.

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Section: Introductionmentioning

confidence: 99%

Current distribution and potential expansion of the harmful benthic dinoflagellateOstreopsiscf.siamensistowards the warming waters of the Bay of Biscay,North‐EastAtlantic

Drouet

Jauzein

Herviot-Heath

et al. 2021

Environmental Microbiology

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“…The spike experiments for all three toxigenic species with an average recovery efficiency of~94% of the target cells over a range of 10 2 to 10 4 target cells per L revealed a very robust assay design and reliability of the quantitative field data calculations. Specific qPCR is thus confirmed to be a valuable tool for the detection and enumeration of low abundant species [77]. In addition to this and previous Amphidomataceae qPCR spike-recovery experiments [32], a direct comparison of qPCR quantification with other independent quantification methods over a larger data set is desirable.…”

Section: Plos Onementioning

confidence: 77%

Distribution and abundance of azaspiracid-producing dinophyte species and their toxins in North Atlantic and North Sea waters in summer 2018

et al. 2020

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Representatives of the marine dinophyte family Amphidomataceae produce lipophilic phycotoxins called azaspiracids (AZA) which may cause azaspiracid shellfish poisoning (AZP) in humans after consumption of contaminated seafood. Three of the four known toxigenic species are observed frequently in the eastern North Atlantic. In 2018, a research survey was performed to strengthen knowledge on the distribution and abundance of toxigenic Amphidomataceae and their respective toxins in Irish coastal waters and in the North Sea. Species-specific quantification of the three toxigenic species (Azadinium spinosum, Azadinium poporum and Amphidoma languida) was based on recently developed qPCR assays, whose performance was successfully validated and tested with specificity tests and spike experiments. The multi-method approach of on-board live microscopy, qPCR assays and chemical AZA-analysis revealed the presence of Amphidomataceae in the North Atlantic including the three targeted toxigenic species and their respective AZA analogues (AZA-1,-2,-33,-38,-39). Azadinium spinosum was detected at the majority of Irish stations with a peak density of 8.3 x 10 4 cells L-1 and AZA (AZA-1,-2,-33) abundances up to 1,274 pg L-1. Amphidoma languida was also present at most Irish stations but appeared in highest abundance in a bloom at a central North Sea station with a density of 1.2 x 10 5 cells L-1 and an AZA (AZA-38,-39) abundances of 618 pg L-1. Azadinium poporum was detected sporadically at the Irish south coast and North Sea and was rather low in abundance during this study. The results confirmed the wide distribution and frequent occurrence of the target species in the North Atlantic area and revealed, for the first time, bloom abundances of toxigenic Amphidomataceae in this area. This emphasizes the importance of future studies and monitoring of amphidomatacean species and their respective AZA analogues in the North Atlantic.

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“…In the sampling areas of this study, both toxic K. veneficum and nontoxic K. zhouanum were detected (Huang et al . ). Questions about how these two closely related species interact with each other, the ecological significance of population dynamics in the field, and whether the potentially nontoxic K. zhouanum supports the growth of the omnivorous mixotrophic K. veneficum should be considered for future studies in the laboratory and in the field.…”

Section: Discussionmentioning

confidence: 97%

Biochemical characteristics support the recently described species Karlodinium zhouanum (Gymnodiniales, Dinophyceae)

Zhu

Zhou

Meng

et al. 2019

Phycological Research

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SUMMARY The small athecate dinoflagellate Karlodinium zhouanum is a species recently described in the coastal waters of China. K. zhouanum is morphologically similar to Karlodinium veneficum, a typical ichthyotoxic blooming karlotoxin‐producing species, and it is impossible to distinguish between these two species based on light microscopy. In this study, strains of K. zhouanum isolated from the East China Sea were studied. By analyzing toxins, toxicity, lipid characteristics and typical molecular and physiological traits of this species, K. zhouanum was shown to be nontoxic to brine shrimp and widely spread over the coastal waters of China. No karlotoxin‐like toxin was detected by liquid chromatography‐mass spectrometry (LC–MS). Instead of gymnodinosterol, the critical sterol in toxic K. veneficum, 27(nor)‐24S‐4α‐Methyl‐5α‐ergosta‐8(14)‐en‐3β‐ol (NEE) was dominant in K. zhouanum, while gymnodinosterol was absent. These sterol characteristics may provide not only support for the species separation between toxic and nontoxic species of Karlodinium but also environmental survey tools to differentiate the contribution of nontoxic Karlodinium strains, which has been unclear until now.

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Distribution of Karlodinium veneficum in the coastal region of Xiangshan Bay in the East China Sea, as detected by a real-time quantitative PCR assay of ribosomal ITS sequence

Cited by 21 publications

References 38 publications

Current distribution and potential expansion of the harmful benthic dinoflagellateOstreopsiscf.siamensistowards the warming waters of the Bay of Biscay,North‐EastAtlantic

Current distribution and potential expansion of the harmful benthic dinoflagellateOstreopsiscf.siamensistowards the warming waters of the Bay of Biscay,North‐EastAtlantic

Distribution and abundance of azaspiracid-producing dinophyte species and their toxins in North Atlantic and North Sea waters in summer 2018

Biochemical characteristics support the recently described species Karlodinium zhouanum (Gymnodiniales, Dinophyceae)

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