Distribution of Laminins in the Developing Human Eye

Byström, Berit; Virtanen, Ismo; Rousselle, Patricia; Gullberg, Donald; Domellöf, Fátima Pedrosa

doi:10.1167/iovs.05-0367

Cited by 63 publications

(59 citation statements)

References 45 publications

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“…In our study, neither the epithelial BM nor the DM were immunoreactive for LM 1 chain, in agreement with Filenius et al (2001) and our previous data showing the disappearance of this chain from the epithelial BM of the human cornea after 12 wg and from the DM after 17 wg (Byström et al 2006). We were not able to detect any speciWc staining in the adult human cornea using a polyclonal antibody against LM 1 chain (Tiger et al 1997), although this antibody worked well in fetal corneal specimens (Byström et al 2006).…”

Section: Laminin 1 Chainsupporting

confidence: 91%

“…LM 4 chain was found in the epithelial BM of two keratoconus cases with scarring. We have previously reported the presence of this chain in developing human corneas at 10-20 wg (Byström et al 2006) and here we show that it is normally downregulated in the adult cornea. The occurrence of LM 4 chain in these two cases further supports our hypothesis of disturbed laminin regulation during development as the pathogenesis of some cases of keratoconus.…”

Section: Keratoconussupporting

confidence: 74%

“…We were not able to detect any speciWc staining in the adult human cornea using a polyclonal antibody against LM 1 chain (Tiger et al 1997), although this antibody worked well in fetal corneal specimens (Byström et al 2006). This is also in line with the known restricted distribution of LM 1 chain in epithelial BMs of fetal and adult human tissues, as it appears to be lacking in BMs of stratiWed epithelia both in man and mice (Falk et al 1999;Virtanen et al 2000).…”

Section: Laminin 1 Chainsupporting

confidence: 70%

“…In cases without LM 3 chain, the molecular composition of the laminin containing 3 2 remains to be elucidated, but the LM 3 and 2 chains might be present as dimers, as previously reported in some cell lines (Hirosaki et al 2000). LM 3 chain is present in the fetal DM at 17-20 weeks of gestation (wg) (Byström et al 2006), but it was not present in normal adult corneas indicating that its expression is developmentally regulated. The occurrence of LM 3 chain in the majority of the DM of keratoconus corneas, might either reXect novel production of this laminin chain as a consequence of keratoconus or it may be due to the absence of normal develop- Cross-sections of the interface between donor and host cornea (arrowheads in a-f) and the DM (arrows in a-c) in a case with scarring after deep lamellar keratoplasty.…”

Section: Keratoconusmentioning

confidence: 83%

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Laminins in normal, keratoconus, bullous keratopathy and scarred human corneas

Byström

Virtanen

Rousselle

et al. 2007

Histochem Cell Biol

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The laminin composition (LMalpha1-alpha5, beta1-beta3, gamma1 and gamma2 chains) of normal corneas and corneal buttons from keratoconus, bullous keratopathy (BKP), Fuchs' dystrophy + BKP, Fuchs' dystrophy without BKP and scar after deep lamellar keratoplasty (DLKP) was investigated with immunohistochemistry. The epithelial basement membranes (BMs) of both normal and diseased corneas contained LMalpha3, alpha5, beta1, beta3, gamma1 and gamma2 chains. The epithelial BM morphology was altered in the different diseases. Scarring was associated with irregular BM and ectopic stromal localization of different laminin chains. The Descemet's membrane (DM) contained LMalpha5, beta1 and gamma1 chains in all cases and additionally LMbeta3 and gamma2 chains in the majority of keratoconus corneas. The interface in the DLKP cornea had patches of LMalpha3, alpha4, alpha5, beta1 and beta2 chains, and an extra BM-like structure under the Bowman's membrane. These results suggest that laminin chains participate in the process of corneal scarring and in the pathogenesis of some corneal diseases. The novel finding of LMalpha3, beta3 and gamma2 in the DM of keratoconus buttons indicates that this membrane is also involved in the disease and that some cases of keratoconus may have a congenital origin, without normal downregulation of the LMbeta3 chain.

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Section: Laminin 1 Chainsupporting

confidence: 91%

Section: Keratoconussupporting

confidence: 74%

Section: Laminin 1 Chainsupporting

confidence: 70%

Section: Keratoconusmentioning

confidence: 83%

See 2 more Smart Citations

Laminins in normal, keratoconus, bullous keratopathy and scarred human corneas

Byström

Virtanen

Rousselle

et al. 2007

Histochem Cell Biol

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“…In addition, an individual basement membrane contains a unique set of more minor proteins. The expression and localization of laminin isoforms have been described in great detail for rat [6] and human [7] retinae, and the distribution of collagen IV isoforms in human Bruch's membrane is also known [8] . The presence of nidogen, presumably nidogen 1, in the retina has been noted, but the relative distribution of nidogen 1 and nidogen 2 has not been studied.…”

Section: Introductionmentioning

confidence: 99%

Deposition of Nidogens and Other Basement Membrane Proteins in the Young and Aging Mouse Retina

Kunze¹,

Abari

Semkova

et al. 2009

Ophthalmic Res

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Aim: To determine the distribution of major basement membrane constituents, particularly nidogen 1 and 2, in young and aging mouse retinae. Methods: The specificity of antibodies against basement membrane proteins was ascertained by immunoblotting with proteins extracted from mouse retinae. The same antibodies were used in indirect immunofluorescence microscopy to localize basement membrane proteins in paraffin sections of retinae from 1-, 12- and 18-month-old C57BL/6 mice. Results: At a young age, laminin, perlecan and collagen IV were most abundant in Bruch’s membrane. Later, the proteins were clearly detected in capillary basement membranes and the inner limiting membrane. In both of these basement membranes, a massive increase in protein amount was seen upon aging, whereas in Bruch’s membrane the staining intensity was less drastically changed. Both nidogen 1 and 2 were present in vascular basement membranes and Bruch’s membrane throughout the age periods studied. In the inner limiting membrane, the nidogens were more strongly expressed at higher ages, with an earlier and more extensive deposition of nidogen 1. Conclusions: All major basement membrane constituents are present in the mouse retina, but the onset of deposition differs among the different proteins and between the various retinal basement membranes. In general, basement membrane protein deposition increases with age.

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New Biomimetic Directions in Regenerative Ophthalmology

Green

Watson

et al. 2012

Adv Healthcare Materials

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One of the most complete and permanent ways of treating many causes of visual impairment and blindness is to replace the entire affected tissue with pre-cultured ocular tissues supported and maintained on biomaterial frameworks. One direction towards enhancing ocular tissue regeneration on biomaterials, in the laboratory is by applying biomimicry. Specifically to engineer biomaterials with important functional elements of the native extracellular matrices, such as topography, that support and organise cells into coherent tissues. Further problems in regenerative ophthalmology can be potentially solved through application of biomimicry. They include, more efficient ways of moving and transplanting cultivated tissues into correct therapeutic locations inside the eye and scar-less, non-destructive healing of surgical incisions and wounds, to repair structural integrity of tissues at the ocular surface. Two examples are given to show this potential for redeveloping an ocular epithelium onto a nanostructured insect wing surface and producing an origami membrane modelled on deployable structures in nature. Efforts to harness natural innovation will eventually provide unique designs and structures that cannot for now be made synthetically, for regeneration of clinically acceptable ocular tissues.

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Distribution of Laminins in the Developing Human Eye

Cited by 63 publications

References 45 publications

Laminins in normal, keratoconus, bullous keratopathy and scarred human corneas

Laminins in normal, keratoconus, bullous keratopathy and scarred human corneas

Deposition of Nidogens and Other Basement Membrane Proteins in the Young and Aging Mouse Retina

New Biomimetic Directions in Regenerative Ophthalmology

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