Distribution of lipids in cytoplasmic and outer membranes of Escherichia coli K12

Lugtenberg, E. J. J.; Peters, R.C.

doi:10.1016/0005-2760(76)90279-4

Cited by 177 publications

(124 citation statements)

References 24 publications

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“…Composition of E. coli Lipid Extracts-The polar head group and acyl chain compositions of the membrane lipids of the wild-type strain K12 used in this investigation are very similar to those reported earlier (Aibara et al, 1972;Cronan and Rock, 1987;Ishinaga et al, 1979;Lugtenberg and Peters, 1976;Marr and Ingraham, 1962;Suutari and Laakso, 1994). The cells responded to a higher growth temperature by synthesizing lipids with shorter and more saturated acyl chains.…”

Section: Discussionsupporting

confidence: 83%

Wild-type Escherichia coli Cells Regulate the Membrane Lipid Composition in a “Window” between Gel and Non-lamellar Structures

Morein

Andersson

Rilfors

et al. 1996

Journal of Biological Chemistry

350

287

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Escherichia coli strain K12 was grown at 17, 27, and 37°C. The acyl chain composition of the membrane lipids varied with the growth temperature; the fraction of cis-vaccenoyl chains decreased, and the fraction of palmitoyl chains increased, when the growth temperature was increased. However, the polar head group composition did not change significantly. The equilibria between lamellar and reversed non-lamellar phases of lipids extracted from the inner membrane (IM), and from both the membranes (IOM), were studied with NMR and x-ray diffraction. At temperatures above the growth temperature the lipid extracts formed a reversed hexagonal phase, or a bicontinuous cubic phase, depending on the degree of hydration of the lipids. It was observed that: 1) at equal elevations above the growth temperature, IM lipid extracts, as well as IOM lipid extracts, have a nearly equal ability to form nonlamellar phases; 2) IM extracts have a stronger tendency than IOM extracts to form non-lamellar phases; 3) nonlamellar phases are formed under conditions that are relatively close to the physiological ones; the membrane lipid monolayers are thus "frustrated"; and 4) as a consequence of the change of the acyl chain structures, the temperature for the lamellar gel to liquid crystalline phase transition is changed simultaneously, and in the same direction, as the temperature for the lamellar to non-lamellar phase transition. With a too large fraction of saturated acyl chains the membrane lipids enter a gel state, and with a too large fraction of unsaturated acyl chains the lipids transform to non-lamellar phases. It is thus concluded that the regulation of the acyl chain composition in wild-type cells of E. coli is necessary for the organism to be able to grow in a "window" between a lamellar gel phase and reversed non-lamellar phases.

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Section: Discussionsupporting

confidence: 83%

Wild-type Escherichia coli Cells Regulate the Membrane Lipid Composition in a “Window” between Gel and Non-lamellar Structures

Morein

Andersson

Rilfors

et al. 1996

Journal of Biological Chemistry

350

287

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“…The results are in good agreement with those previously published [4,12,15]. These extracted lipids were studied by NMR and freeze-fracture electron microscopy.…”

Section: Characterization Of Isolated Total Phospholipidssupporting

confidence: 91%

“…After conversion of the cells to spheroplasts with lysozyme and EDTA, membranes were isolated and separated according to a procedure [4] basically consisting of that of Osborn et al [3], modified in that the spheroplasts were lysed after centrifugation by resuspending in buffer without sucrose. The quality of the separation appeared to be good [9].…”

Section: Isolation and Analysis Of The Cytoplasmic Membranementioning

confidence: 99%

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31P nuclear magnetic resonance and freeze-fracture electron microscopy studies on Escherichia coli. I. Cytoplasmic membrane and total phospholipids

Burnell

Alphen

Verkleij

et al. 1980

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Summary1. At the growth temperature the total phospholipids isolated from Escherichia coli cells give rise to 31p-NMR spectra which indicate the existence of lamellar, isotropic and hexagonal phases. These phases are also detected by freeze-fracture electron microscopy. In particular, the isotropic phase may contain lipidic particles (possibly inverted micelles) associated with the lamellar phase.2. The cytoplasmic membrane isolated from E. coli cells grown at 37°C is mainly lamellar at 25°C, whereas at 37 and 45°C the presence of some almost isotropic phospholipid motion is indicated. The possible significance of the isotropic phase for the functioning of the cytoplasmic membrane is discussed.

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“…Also the phospholipid composition differs between these membranes. For example, the outer leaflet of erythrocyte membranes is dominated by zwitterionic lipids, such as phosphatidylcholine and sphingomyelin, thus being 4 essentially uncharged (8). In contrast, bacterial membranes are rich in anionic lipids.…”

Section: Bacterial Membranes As Amp Targetsmentioning

confidence: 99%

(Lipo)polysaccharide interactions of antimicrobial peptides

Schmidtchen

Malmsten

2015

Journal of Colloid and Interface Science

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Due to rapidly increasing resistance development against conventional antibiotics, as well as problems associated with diseases either triggered or deteriorated by infection, antimicrobial and anti-inflammatory peptides have attracted considerable interest during the last few years. While there is an emerging understanding of the direct antimicrobial function of such peptides through bacterial membrane destabilization, the mechanisms of their anti-inflammatory function are less clear. We here summarize some recent results obtained from our own research on antiinflammatory peptides, with focus on peptide-(lipo)polysaccharide interactions.3

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Distribution of lipids in cytoplasmic and outer membranes of Escherichia coli K12

Cited by 177 publications

References 24 publications

Wild-type Escherichia coli Cells Regulate the Membrane Lipid Composition in a “Window” between Gel and Non-lamellar Structures

Wild-type Escherichia coli Cells Regulate the Membrane Lipid Composition in a “Window” between Gel and Non-lamellar Structures

31P nuclear magnetic resonance and freeze-fracture electron microscopy studies on Escherichia coli. I. Cytoplasmic membrane and total phospholipids

(Lipo)polysaccharide interactions of antimicrobial peptides

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