Distribution of main plant-parastic nematodes in sweet potato and taro fields in Kyushu and Okinawa, Japan. 1. Survey in the central and southern parts in Kyushu Island (Kumamoto,Miyazaki and Kagoshima Prefs.) and development of an effective DNA analysis method for species identification.

Iwahori, Hideaki; Sano, Zen-ichi; Ogawa, Tetsuji

doi:10.4241/kyubyochu.46.112

Cited by 33 publications

(15 citation statements)

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“…DNA was extracted from individual nematodes according to the method of Iwahori et al (2000) with slight modifications. A nematode was put into a drop of water on a glass slide, air-dried and cut with a sterile needle under a microscope.…”

Section: Dna Extraction From Nematodesmentioning

confidence: 99%

Development of specific primers to Hirschmanniella spp. causing damage to lotus and their economic threshold level in Tokushima prefecture in Japan

Koyama

Thar

Kizaki

et al. 2013

Nematol

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Nematode diseases caused by Hirschmanniella spp. are becoming a major threat to lotus production in Tokushima Prefecture, a primary production area in Japan. The objectives of this study were: /) to design specific primers to Hirschmanniella spp. causing damage to lotus; ii) to evaluate the relationship between pre-plant density of Hirschmanniella spp. in soil and damage index of lotus; and Hi) to establish an economic threshold level. Since two species, Hirschmanniella imamuri and H. diversa, are known as nematode pests on lotus, three real-time PCR primers were designed based on the ITS regions specific to H. imamuri (designated as imaF and imaR), H. diversa (divF and divR), and both species (HdiF and HdiR). The primers imaF and imaR did not react to DNA of nematodes extracted from lotus fields in Tokushima but the primers divF and divR did, suggesting that the damage seen in Tokushima is mainly caused by H. diversa. A calibration curve was made to evaluate the relationship between the number of Hirschmanniella spp. inoculated to soil and the threshold cycle values. The quantification of Hirschmanniella spp. in soil was conducted by real-time PCR method with the primers HdiF and HdiR as well as the Baermann method. The economic threshold level was estimated as ten individuals of Hirschmanniella spp. (100 g fresh soil)"' with the Baermann method and 50 individuals (100 g oven-dried soil)"' with real-time PCR.

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Section: Dna Extraction From Nematodesmentioning

confidence: 99%

Development of specific primers to Hirschmanniella spp. causing damage to lotus and their economic threshold level in Tokushima prefecture in Japan

Koyama

Thar

Kizaki

et al. 2013

Nematol

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“…Primer specificity was evaluated using M. hapla and M. incognita with real-time PCR. DNA was extracted from individual J2s, according to the method of Iwahori et al (2000) with slight modifications. A nematode was put into a drop of water on a glass slide, air-dried and cut with a sterile needle under a microscope.…”

Section: Primersmentioning

confidence: 99%

Development of a direct quantitative detection method for Meloidogyne incognita and M. hapla in andosol and analysis of relationship between the initial population of Meloidogyne spp. and yield of eggplant in an andosol

Watanabe

Masumura

Kioka

et al. 2013

Japanese Journal of Nematology

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Baermann method, while that of M. incognita and M. hapla from 0.6 to 713 J2 equivalent (J2eq)/20 g soil and from 0.0 to 115 J2eq/20 g soil, respectively, with the real-time PCR method. The yield was determined by the sum of commercial sized eggplants harvested for 3 months of the cultivation period. The yield decreased in the plots with an initial population of RKNs more than 2 J2/20 g soil with the Baermann method. In real-time PCR, the yields were low in the plots with the sum of initial M. incognita and M. hapla more than 128 J2eq/20 g soil. The present study established a quantification method with real-time PCR for M. incognita and M. hapla in andosol and evaluated the relationship between the initial population of Meloidogyne spp. and the yield of eggplant. Nematol. Res. 43(2), 21-29 (2013)

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“…are very common in Japan, and some species are frequently detected together in a single field. [14][15][16][17] If such virulent species are latent in a field, the known resistant cultivars described above could not suppress the infestation of RKN. The importance of the identification of new Solanum sources that carry multiple resistance genes or broad-spectrum resistance to Meloidogyne spp.…”

Section: Introductionmentioning

confidence: 99%

“…27,28 The RKN species were confirmed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. 15 All isolates were established from a single eggmass and propagated on glasshouse-grown tomato (S. lycopersicum 'Pritz') or eggplant (S. melongena 'Senryo Nigo'). Eggs collected from tomato or eggplant roots were incubated at 25°C…”

Section: Introductionmentioning

confidence: 99%

Solanum palinacanthum: broad‐spectrum resistance to root‐knot nematodes (Meloidogyne spp.)

Murata

Uesugi

Uehara

et al. 2020

Pest Management Science

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BACKGROUND: Root-knot nematodes (RKN, Meloidogyne spp.) are harmful phytophagous pests of Solanum spp. Some RKN species are becoming worldwide problems because of their virulence to RKN-resistant Solanum species. A new Solanum species carrying broad-spectrum resistance to Meloidogyne spp. is required for the effective management of this pest. Here we sought to determine the host suitability of RKN to Solanum palinacanthum, a wild Solanum species, and to evaluate its potential effectiveness in RKN management. RESULTS: We identified an RKN-resistant Solanum species, S. palinacanthum, by screening Solanum accessions. We tested its spectrum of resistance to common Meloidogyne spp. in Japan. In pot tests inoculated with second-stage juveniles, S. palinacanthum showed poor host suitability for Melidogyne incognita, M. arenaria genotypes A2-J and A2-O, M. javanica and M. hapla, indicating broad-spectrum resistance to RKN. The development of M. incognita within S. palinacanthum roots was significantly poorer than that in susceptible S. melongena and S. lycopersicum at 10 and 21 days after inoculation. Microplot tests confirmed that the number of second-stage juveniles in plots where S. palinacanthum grew and root galling of the root system were significantly lower than those of susceptible S. melongena, suggesting that the resistance could be used to manage RKN under field conditions. CONCLUSION: S. palinacanthum showed poor host suitability to all Meloidogyne spp. tested in this study, and it thus has the potential to be used as a genetic resource with broad-spectrum RKN resistance, and it could be effective against multiple RKN species in a field.

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Distribution of main plant-parastic nematodes in sweet potato and taro fields in Kyushu and Okinawa, Japan. 1. Survey in the central and southern parts in Kyushu Island (Kumamoto,Miyazaki and Kagoshima Prefs.) and development of an effective DNA analysis method for species identification.

Cited by 33 publications

References 0 publications

Development of specific primers to Hirschmanniella spp. causing damage to lotus and their economic threshold level in Tokushima prefecture in Japan

Development of specific primers to Hirschmanniella spp. causing damage to lotus and their economic threshold level in Tokushima prefecture in Japan

Development of a direct quantitative detection method for Meloidogyne incognita and M. hapla in andosol and analysis of relationship between the initial population of Meloidogyne spp. and yield of eggplant in an andosol

Solanum palinacanthum: broad‐spectrum resistance to root‐knot nematodes (Meloidogyne spp.)

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