Distribution of MAP1A, MAP1B, and MAP2A&amp;B during layer formation in the optic tectum of developing chick embryos

Yasuda, Yuko; Fujita, Setsuya

doi:10.1007/s00441-003-0796-z

Cited by 7 publications

(2 citation statements)

References 30 publications

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“…We found that microtubule-associated protein 2 (MAP2), a neuronal marker, was also expressed in layers IV/V ( Fig. 1D-F; Yasuda and Fujita, 2003). Expression of Sema3A, a ligand for NRP1, was localized in layer VI (Fig.…”

Section: Sema3a and Nrp1 Are Expressed In Distinct Tectal Layersmentioning

confidence: 86%

NRP1-mediated Sema3A signals coordinate laminar formation in the developing chick optic tectum

2014

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The optic tectum comprises multiple layers, which are formed by radial and tangential migration during development. Here, we report that Neuropilin 1 (NRP1)-mediated Sema3A signals are involved in the process of tectal laminar formation, which is elaborated by tangential migration. In the developing chick tectum, NRP1, a receptor for Sema3A, is expressed in microtubule-associated protein 2 (MAP2)-positive intermediate layers IV and V. Sema3A itself is a diffusible guidance factor and is expressed in the overlying layer VI. Using stable fluorescent labeling of tectal cells, we show that MAP2-positive intermediate layers are formed by the neurons that have been dispersed by tangential migration along the tectal efferent axons. When Sema3A was mis-expressed during laminar formation, local Sema3A repelled the tangential migrants, thus eliminating MAP2-positive neurons that expressed NRP1. Furthermore, in the absence of the MAP2-positive neurons, tectal layers were disorganized into an undulated form, indicating that MAP2-positive intermediate layers are required for proper laminar formation. These results suggest that NRP1-mediated Sema3A signals provide repulsive signals for MAP2-positive neurons to segregate tectal layers, which is important in order to coordinate laminar organization of the optic tectum.

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Section: Sema3a and Nrp1 Are Expressed In Distinct Tectal Layersmentioning

confidence: 86%

NRP1-mediated Sema3A signals coordinate laminar formation in the developing chick optic tectum

2014

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“…Live imaging in these neuro-2a cells shows that MAP2c triggers neurite formation through rapid accumulation and bundling of stable MAP2c bound microtubules, and in hippocampal neurons loss of either the MAP2c microtubule binding domain or the protein kinase A binding domain of MAP2c impairs neurite formation (Dehmelt et al, 2006). The expression of the higher molecular weight MAP2a and MAP2b isoforms generally occurs after neurite growth and during neuron maturation, however their expression has been observed in developing neuroblasts in the optic tectum preceding differentiation into multipolar cells, but the roles of MAP1a and MAP2b with respect to neurite initiation in this case are unknown (Matus et al, 1990; Schoenfeld and Obar, 1994; Yasuda and Fujita, 2003). …”

Section: Regulation Of Microtubules By Mapsmentioning

confidence: 99%

Cytoskeletal and signaling mechanisms of neurite formation

Sainath

Gallo

2014

Cell Tissue Res

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The formation of a neurite, the basis for axons and dendrites, begins with a concerted accumulation and organization of actin and microtubules. While much is known about proteins that play a role in these processes, as they perform similar functions in axon branching and filopodia formation, much remains to be discovered how individual cytoskeletal regulators interact to form a neurite. Here we review the literature regarding different models of filopodial formation and how proteins that control actin organization and polymerization induce neurite formation. While several different regulators of actin polymerization play roles in neurite initiation there is redundancy between these regulators, as the effects of loss of a single regulator can be mitigated by addition of neurite promoting substrates and proteins. Similar to actin dynamics, both microtubule stabilizing and destabilizing proteins play a role in neurite initiation. Furthermore, interactions between the actin and microtubule cytoskeleton are required for neurite formation. Several lines of evidence indicate that the interactions between these two components of the cytoskeleton are needed for force generation as well as localization of microtubules at sites of nascent neurites. The general theme that emerges is the existence of several central regulatory pathways that extracellular cues converge on which can control and organize both actin and microtubules to induce the formation of neurites.

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The chick optic tectum developmental stages. A dynamic table based on temporal‐ and spatial‐dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis

Rapacioli¹,

Celin²,

Duarte³

et al. 2011

Journal of Morphology

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Development is often described as temporal sequences of developmental stages (DSs). When tables of DS are defined exclusively in the time domain they cannot discriminate histogenetic differences between different positions along a spatial reference axis. We introduce a table of DSs for the developing chick optic tectum (OT) based on time- and space-dependent changes in quantitative morphometric parameters, qualitative histogenetic features and immunocytochemical pattern of several developmentally active molecules (Notch1, Hes5, NeuroD1, β-III-Tubulin, synaptotagmin-I and neurofilament-M). Seven DSs and four transitional stages were defined from ED2 to ED12, when the basic OT cortical organization is established, along a spatial developmental gradient axis extending between a zone of maximal and a zone of minimal development. The table of DSs reveals that DSs do not only progress as a function of time but also display a spatially organized propagation along the developmental gradient axis. The complex and dynamic character of the OT development is documented by the fact that several DSs are simultaneously present at any ED or any embryonic stage. The table of DSs allows interpreting how developmental cell behaviors are temporally and spatially organized and explains how different DSs appear as a function of both time and space. The table of DSs provides a reference system to characterize the OT corticogenesis and to reliably compare observations made in different specimens.

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Distribution of MAP1A, MAP1B, and MAP2A&B during layer formation in the optic tectum of developing chick embryos

Cited by 7 publications

References 30 publications

NRP1-mediated Sema3A signals coordinate laminar formation in the developing chick optic tectum

NRP1-mediated Sema3A signals coordinate laminar formation in the developing chick optic tectum

Cytoskeletal and signaling mechanisms of neurite formation

The chick optic tectum developmental stages. A dynamic table based on temporal‐ and spatial‐dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis

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