Distribution of Menaquinones in Actinomycetes and Corynebacteria

Abstract

SUMMARYMenaquinones were the only isoprenoid quinones found in 48 corynebacteria and actinomycete strains examined. Dihydromenaquinones having nine isoprene units were the main components isolated from Gordona, Mycobacterium, Corynebacterium bovis, Corynebacterium glutamicum and a strain labelled Nocardia farcinica, but dihydromenaquinones having eight isoprene units were characteristic of other Corynebacterium species and representatives of the ' rhodochrous' complex. Tetrahydromenaquinones having six and eight isoprene units were found in Nocardia strains and in a single strain of Micropolyspora brevicatena, which also contained mycolic acids similar in chain length to those of Nocardia. Menaquinones having nine isoprene units with from one to five double bonds hydrogenated were the main Components in Actinomadura madurae, Actinomadura pelletieri, Micropolyspora faeni, Oerskovia turbata and Streptomyces strains. Actinomadura dassonvillei strains had a characteristic pattern of di-, tetra-and hexahydromenaquinones with 10 isoprene units which was slightly different from the pattern in mixtures of similar quinones from Actinomyces israelii and A c t inom y ces viscosus .

Phenatic Acids A and B, New Potentiators of Antifungal Miconazole Activity Produced by Streptomyces sp. K03-0132

“…The type of diaminopimelic acid (DAP) isomers was determined by the method of Becker et al [11]. Menaquinones were extracted and purified by the methods of Collins et al [12], then analyzed by HPLC equipped with a CAPCELL PAK C18 column (Shiseido) [13].…”

Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins

“…Biomass for the molecular systematics and chemotaxonomic studies was obtained after cultivation in yeast extract-glucose broth (yeast extract 1.0%, glucose 1.0%, pH 7.0) for 1 week at 27 1C. After extraction of isoprenoid quinones, as described by Collins et al, 26 analysis was done by HPLC (Agilent 1100, Agilent, Santa Clara, CA, USA) and mass spectrometer (JEOL JMS-T 100LP) using a Pegasil ODS column (Senshu, Tokyo, Japan), according to Tamaoka et al 27 Isomers of diaminopimelic acid were determined by TLC using whole-cell hydrolysates. 28 The N-acyl types of muramic acid were determined by the method of Uchida and Aida.…”

Retymicin, Galtamycin B, Saquayamycin Z and Ribofuranosyllumichrome, Novel Secondary Metabolites from Micromonospora sp. Tü 6368

“…Diaminopimelic acid [11] and the composition of cell-wall sugars [12] was identified by TLC of the whole-organism hydrolysate. Fatty acids were determined by GC-MS [13,14], and menaquinones by reversed-phase HPLC [15,16]. 16S rRNA gene amplification and determination of the almost complete sequence (1495 bp) were carried out according to Rainey et al (1996) [17].…”