Distribution of MICB diversity in the Zhejiang Han population: PCR sequence-based typing for exons 2–6 and identification of five novel MICB alleles

Ying, Yanling; He, Yanmin; Tao, Sudan; Han, Z.‐D.; Wang, Wei; Chen, Nanying; He, J.; Zhang, Wei; He, Ji; Zhu, Faming; Lv, H.-J.

doi:10.1007/s00251-013-0699-4

Cited by 14 publications

(15 citation statements)

References 31 publications

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“…Compared to MICA locus, MICB allelic distribution has been less thoroughly investigated in worldwide populations [6]. Our data showed that similar to Chinese Han populations and European Caucasian populations studied [6,[13][14][15][16], MICA*008:01 and MICB*005:02 were the most common alleles in this population. Peculiarities in MICA allelic profile were observed in this population, for example this population had a relatively high frequency of MICA gene deletion (5.7%).…”

Section: Discussionmentioning

confidence: 54%

“…Nowadays most Chinese Mongolians inhabit the Inner Mongolia autonomous region, the third largest subdivision of China covering about 12.3% of the country's territory, where Mongols are the second largest ethnic group comprising about 17% (approximately 4 million) of the population. Data on MICA and MICB polymorphisms and their linkage disequilibrium (LD) with HLA-B in different Chinese Han populations are accumulating [13][14][15][16]; nevertheless, such information is still lacking for the Chinese Mongolian population. In this study, we investigated MICA and MICB polymorphisms as well as their LD with HLA-B in a Chinese Mongolian population.…”

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confidence: 99%

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MICA,MICB Polymorphisms and Linkage Disequilibrium with HLA‐B in a Chinese Mongolian Population

et al. 2016

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In this study, polymorphisms of major histocompatibility complex class I chain-related genes A and B (MICA and MICB) and human leucocyte antigen (HLA)-B gene were investigated for 158 unrelated Chinese Mongolian subjects recruited from central Inner Mongolia Autonomous Region, northern China, by polymerase chain reaction-sequence-based typing (PCR-SBT) and cloning. Collectively, 79 alleles, including 20 MICA, 12 MICB and 47 HLA-B alleles, were identified. MICA*008:01 (21.2%), MICB*005:02 (48.1%) and HLA-B*51:01 (7.91%) were the most common alleles. Significant global linkage disequilibrium (LD) was detected between HLA-B and MICA, HLA-B and MICB, and MICA and MICB loci (all P < 0.000001). The most frequent haplotypes were HLA-B*51:01-MICA*009:01 (7.28%), HLA-B*58:01-MICB*008 (6.96%), MICA*010-MICB*005:02 (13.92%) and HLA-B*58:01-MICA*002:01-MICB*008 (6.96%). HLA-B-MICA haplotypes such as HLA-B*50:01-MICA*009:02 were associated with single MICB allele. Some HLA-B-MICA haplotypes were associated with multiple MICB alleles, including HLA-B*51:01-MICA*009:01. One novel MICB allele, MICB*031, was identified, which has possibly arisen from MICB*002:01 through single mutation event. We also confirmed the existence of a recently recognized MICA allele, MICA*073, whose ethnic origin has not been previously described. Genotype distributions at MICA, MICB and HLA-B were consistent with a neutrality model. Our results provide new insight into MIC genetic polymorphisms in Chinese ethnic groups. Findings shown here are important from an anthropologic perspective and will inform future studies of the potential role of MIC genes in allogeneic organ transplantation and HLAlinked disease association in populations of related ancestry.

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Section: Discussionmentioning

confidence: 54%

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confidence: 99%

MICA,MICB Polymorphisms and Linkage Disequilibrium with HLA‐B in a Chinese Mongolian Population

et al. 2016

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“…There are currently 40 MICB alleles listed in the April 2014 IMGT/HLA Database release . Among these, MICB*005:02 is the most frequent in several populations (35%–65%) followed by MICB*004:01 (12%–26%), MICB*002:01 (11%–16%) and MICB*008 (6%–15%) . MICB allele typing of exons 2–6 by SBT revealed 10 different alleles from 38 IHW cell line DNA samples.…”

Section: Resultsmentioning

confidence: 99%

“…Exon 1 is not included due to an extremely long intron 1 sequence that would render the fragment too large for regular genomic polymerase chain reaction (PCR) amplification. Exons 2–6 of the MICB gene were amplified and sequenced using the methods described by Ying et al and Field et al using primers listed in Table S1. Allele level assignment (excluding intron variants) of MICB alleles was achieved in all cases using Assign SBT™ as above.…”

Section: Methodsmentioning

confidence: 99%

Diversity and characterization of polymorphic 5′ promoter haplotypes of MICA and MICB genes

Cox

Madrigal

2014

Tissue Antigens

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The major histocompatibility complex (MHC) class I-related chain A (MICA) and B (MICB) are ligands for the natural killer group 2, member D (NKG2D) activating receptor expressed on natural killer (NK) cells, natural killer T (NKT) cells, CD8+ T cells and γδ T cells. Natural killer group 2, member D (NKG2D) ligand expression is stress-related and upregulated by infected or oncogenic cells leading to cytolysis. MICA and MICB genes display considerable polymorphism among individuals and studies have investigated allelic association with disease and relevance of MICA in transplantation, with variable success. It is now known that promoters of MICA and MICB are polymorphic with some polymorphisms associating with reduced expression. We sequenced International Histocompatibility Workshop (IHW) cell line DNA to determine promoter types and alleles encoded by exons 2-6. We found 8 of 12 known MICA promoter polymorphisms and although promoter P7 dominated, other promoters associated with the same allele. For example, MICA*002:01 had promoters P3, P4 or P7 and the common MICA*008:01/04 type had P1, P6 or P7. Similarly, we sequenced 8 of 12 known MICB promoter haplotypes. Some coding region defined MICB alleles had a single promoter, for example, MICB*002:01 and promoter P9, whereas the promiscuous MICB*005 allele had promoters P1, P2, P5, P6, P10 or P12. The results indicate potential for variation in expression of MICA and MICB ligands between individuals with the same allelic types. If differential expression by polymorphic MICA and MICB promoters is confirmed by functional studies, involvement of these genes in disease susceptibility or adverse transplantation outcomes may require knowledge of both promoter and allelic types to make meaningful conclusions.

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“…There, the allele MICB * 005 is the most common allele with frequencies of over 50%. It is followed by MICB * 002 and MICB * 004 with frequencies over 10% and MICB * 008 and the null allele MICB * 009N with frequencies over 5% (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

High-Throughput MICA/B Genotyping of Over Two Million Samples: Workflow and Allele Frequencies

et al. 2020

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MICA and MICB are ligands of the NKG2D receptor and thereby influence NK and T cell activity. MICA/B gene polymorphisms, expression levels and the amount of soluble MICA/B in the serum have been linked to autoimmune diseases, infections, and cancer. In hematopoietic stem cell transplantation, MICA matching between donor and patient has been correlated with reduced acute and chronic graft-vs.-host disease and improved survival. Hence, we developed an extremely cost-efficient high-throughput workflow for genotyping MICA/B for newly registered potential stem cell donors. Since mid-2017, we have genotyped over two million samples using NGS amplicon sequencing for MICA/B exons 2-5. In donors of German origin, MICA * 008 is the most common MICA allele with a frequency of 42.3%. It is followed by MICA * 002 (11.7%) and MICA * 009 (8.8%). The three most common MICB alleles are MICB * 005 (43.9%), MICB * 004 (21.7%), and MICB * 002 (18.9%). In general, MICB is less diverse than MICA and only 6 alleles, instead of 15, account for a cumulative allele frequency of 99.5%. In 0.5% of the samples we observed at least one allele of MICA or MICB which has so far not been reported to the IPD/IMGT-HLA database. By providing MICA/B typed voluntary donors, clinicians become empowered to include MICA/B into their donor selection process to further improve unrelated hematopoietic stem cell transplantation.

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Distribution of MICB diversity in the Zhejiang Han population: PCR sequence-based typing for exons 2–6 and identification of five novel MICB alleles

Cited by 14 publications

References 31 publications

MICA,MICB Polymorphisms and Linkage Disequilibrium with HLA‐B in a Chinese Mongolian Population

MICA,MICB Polymorphisms and Linkage Disequilibrium with HLA‐B in a Chinese Mongolian Population

Diversity and characterization of polymorphic 5′ promoter haplotypes of MICA and MICB genes

High-Throughput MICA/B Genotyping of Over Two Million Samples: Workflow and Allele Frequencies

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