Distribution of microtubule-associated protein 2 in the nervous system of the rat studied by immunofluorescence

Camilli, Pietro De; Miller, Peter; Navone, Francesca; Theurkauf, William E.; Vallee, R. B.

doi:10.1016/0306-4522(84)90194-5

Cited by 368 publications

(158 citation statements)

References 33 publications

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“…Within each pyramidal neuron, the MAP2 was localized primarily to the soma and dendrites, whereas the NF200k protein was distributed predominantly to the axon (not shown). Consistent with previous in vivo and in vitro reports (Bernhardt and Matus, 1984;Caceres et al, 1984;De Camilli et (Willins et al, 1997;Jakab and Goldman-Rakic, 1998;Cornea-Hebert et al, 1999). Within the dendrites of cortical pyramidal neurons, the majority of the 5-HT 2A receptors are found intracellularly in close association with MAP-1A (Cornea-Hebert et al, 2002), with a small population of 5-HT 2A receptors also present in dendritic spines and post-synaptic densities (Miner et al, 2003).…”

Section: Resultssupporting

confidence: 90%

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

et al. 2003

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Section: Resultssupporting

confidence: 90%

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

et al. 2003

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“…In sham controls, the distribution of MAP2 im munoreactivity was similar to that described previ ously (Bernhardt and Matus, 1984;DeCamilli et al, 1984). Within the hippocampal formation, basal and apical dendrites of pyramidal cells, as well as den drites of granule cells and dendrites in the CA3c region were intensely stained, whereas perikarya of hippocampal neurons appeared only lightly stained (Fig.…”

Section: Map2supporting

confidence: 79%

Alterations in τ Immunostaining in the Rat Hippocampus following Transient Cerebral Ischemia

Geddes¹,

Schwab²,

Craddock³

et al. 1994

J Cereb Blood Flow Metab

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Summary: Previous studies in gerbils have shown that cytoskeletal disruption and a loss of the dendritic micro tubule-associated protein, MAP2, may occur after short periods of transient global ischemia. T, a predominantly axonal microtubule-associated protein, has not been ex amined following ischemia. We compared neuronal dam age with alterations in MAP2, T, and 72-kD heat shock protein (HSP72) immunostaining at various reperfusion times following 20 min of ischemia in the rat four-vessel occlusion model. T accumulated in neuronal cell bodies throughout the hippocampal formation 30 min to 2 h after the ischemic insult. Perikaryal T immunostaining was transient in most regions, but persisted in polymorphic hilar neurons. This was accompanied by a loss of immu no staining in the target of many hilar neurons, the inner Transient global ischemia is followed by a de layed loss of neurons in select brain regions, includ ing the hippocampus (for review see Schmidt Kastner and Freund, 1991). Using the rat four vessel occlusion model (Pulsinelli and Brierly, 1979), ischemia maintained for 20 min in halothane anesthetized animals results in neuronal damage in CAl and hilus, but not in CA3 (Jorgensen and Di emer, 1982; Pulsinelli et aI., 1982). The postisch emic rate of cell death is not uniform, with hilar neurons degenerating within a few hours, but CAl pyramidal cells degenerating 24-72 h following isch emia and reperfusion (Crain et aI., 1988; Hsu and Received August 3, 1993; final revision received October 28, 1993; accepted November 23, 1993. Address correspondence and reprint requests to Dr. James W. Geddes, 209 Sanders-Brown Building, University of Kentucky, Lexington, KY 40536-0230, U. S. A.Abbreviations used : HSP, heat shock protein; KA, kainic acid; MAP, microtubule-associated protein; TBS, Tris-buffered saline; 4VO, four-vessel occlusion. 554 molecular layer of the dentate gyrus. The same neuronal populations that exhibited increased T immunostaining of perikarya later displayed an induction of HSP72 immu noreactivity. In contrast, loss of MAP2 immunostaining was not consistently observed before neuronal death and did not correspond to HSP72 induction. The altered T immunostaining is not the direct result of excitotoxic in sult, as intrahippocampal injection of kainic acid did not cause the somal accumulation of T, but did cause disrup tion of MAP2 immunostaining. Taken together, the re sults suggest that the somal accumulation of T is an early, sensitive, and selective marker of ischemic insult.

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“…MAPi is, according to our observations, associated exclusively with neurons in the adult brain and is more abundant in dendrites than in axons (11)(12)(13). MAP2 is also neuron specific and is predominantly, and probably exclusively, located in dendrites (12, [14][15][16][17][18]. MAP3 occurs in both neurons and glia, but in neurons it is restricted to neurofilament-rich axons (10,19).…”

supporting

confidence: 52%

Differential expression of distinct microtubule-associated proteins during brain development.

Riederer¹,

Matus²

1985

Proc. Natl. Acad. Sci. U.S.A.

214

169

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The levels of three different microtubuleassociated proteins (MAP1, -2, and -3) in brain were found to undergo large changes during postnatal development. MAPi was barely detectable at birth but thereafter steadily increased, reaching adult levels by postnatal day 20 (P20). Both MAP2 and MAP3 showed differential expression patterns of their component peptides. At birth, MAP2 was represented by the smaller of two Mr 280,000 peptides (MAP2b) and three antigenically related Mr 70,000 peptides. The larger of the M, 280,000 peptides (MAP2a) first appeared between P10 and P20, and the Mr 70,000 components disappeared at the same time. Of the two MAP3 peptides, the larger (MAP3a) was present in the late embryo, several days before MAP3b appeared. Between P10 and P20, both MAP3 components underwent a striking decrease in abundance (a factor of 10), which correlated with their disappearance from all neuronal compartments except neurofrlament-containing axons. These developmental changes in expression are different and characteristic for each of the three MAPs, yet in each case they are detectable in brain homogenates, indicating that they occur concurrently throughout the brain.The assembly of microtubules is an essential step in the growth of neuronal processes (1-3). Although very little is known about its regulation in the developing nervous system, it is believed that microtubule-associated proteins (MAPs) are involved (4-9). Three MAP species have been shown by immunohistochemistry to be differentially distributed in the brain, differing both in the types of cells in which they occur and in their distribution within the neuronal cytoplasm (10). MAPi is, according to our observations, associated exclusively with neurons in the adult brain and is more abundant in dendrites than in axons (11-13). MAP2 is also neuron specific and is predominantly, and probably exclusively, located in dendrites (12, 14-18). MAP3 occurs in both neurons and glia, but in neurons it is restricted to neurofilament-rich axons (10,19). In a recent immunohistochemical study, we followed the appearance of these proteins in the developing rat cerebellum and found that each exhibits a characteristic developmental pattern (26). MAPi appears in axons before dendrites, but the balance changes during the first 3 postnatal weeks by the gradual accumulation of MAPi in dendrites and by its simultaneous decrease in axons. MAP2 is exclusively associated with dendrites throughout development. MAP3 appears transitorily in nascent axons (from which it is absent in mature tissue) and later appears in glial cell bodies and processes.These changes in cellular distribution indicate that the expression of these proteins is developmentally regulated. In the present study, we have used monoclonal antibodies to trace the appearance of MAPi, -2, and -3 in rat brain from the late embryo to the end of the third postnatal week. We find that each MAP shows a distinct developmental pattern, which includes large changes in the abundance of their components that occ...

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Distribution of microtubule-associated protein 2 in the nervous system of the rat studied by immunofluorescence

Cited by 368 publications

References 33 publications

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

Alterations in τ Immunostaining in the Rat Hippocampus following Transient Cerebral Ischemia

Differential expression of distinct microtubule-associated proteins during brain development.

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