“…They were incubated with 1/600 rabbit antimouse laminin, 1/250 rabbit anti-human callus keratin, 1/ 6000 rabbit anti-rat milk fat globule membrane after prior treatment with pronase (Warburton et al, 1982), with 1/50 horseradish peroxidase-conjugated peanut lectin after prior treatment with neuraminidase (Newman et al, 1979) or with 1/250 mouse monoclonal antibody (MAb) to smooth muscle actin (Cambridge BioScience, Cambridge: C34931), 1/50 MAb to vimentin (Dako Ltd, Bucks: M0725), 1/750 rabbit anti-rat recombinant S100A4 (p9Ka) (Gibbs et al, 1995). The primary antibodies were detected using commercially-available antibody complexes containing horseradish peroxidase (Hsu et al, 1981) (Dako Ltd, Bucks), with the appropriate anti-rabbit or anti-mouse biotinylated secondary antibodies (Amersham Int, Bucks), as described previously (Gibbs et al, 1995).…”