Distribution of Na+-K+-activated ATPase in the alimentary tract of Locusta migratoria

Peacock, A. J.

doi:10.1016/0020-1790(76)90080-9

Cited by 13 publications

(4 citation statements)

References 14 publications

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“…However, inconsistent results, as reported here and elsewhere (e.g. Komnick & Achenbach, 1979;Peacock, 1976), suggest that the Ernst (1972b) technique may not be a reliable method for the localization of (Na+ + K+)-ATPase in the Malpighian tubules of Locusta rnigraforia, and some other epithelia. The biochemical experiments also call into question the validity of this technique in the localization of (Na" + K+)-ATPase.…”

Section: Potassium-dependent Phosplzatase In Locusta 139contrasting

confidence: 71%

“…In contrast, the absence of a significant reduction in the amount of precipitate associated with the apical microvilli suggests that relatively little (Na+ + K+)-ATPm activity is associated with the apical cell membrane. These findings are consistent with the fact that, with the exception of frog choroid plexus (Quinton et ul.,197% (Na+ + K+)-ATPase has been reported to be almost exclusively associated with the cytoplasmic side of the lateral and basal cell membrane infoldings of secretory epithelia (Ernst, 1972b;Ernst & Mills, 1977;Karnaky et a/., 1976;Peacock, 1976;Komnick & Achenbach, 1979: GreenWald et al, 1984. Nevertheless, on the basis of the present cytochemical study it is not possible to discount So, (Na+ + Kf)-ATPase activity associated with the aP microvilli.…”

Section: Discussionmentioning

confidence: 53%

“…E. Fogg, Dcpartment of Biological cnces, University of Durham, Scicncc Laboratories, South h d , Durham DHl3LE. Anstee et al, 1986); an enzyme which has been implicated in mechanisms associated with cation and fluid transport processes in various tissues from a variety of different species (Ernst, 1972b;Ernst & Mills, 1977;Karnaky et a[., 1976;Peacock, 1976;Komnick & Achenbach, 1979;Greenwald et a/., 1984). In addition, a K'-activated ATPase has been proposed for the apical surface (Harvey et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

“…Ernst, 1972a, b;Leuenberger & Novikoff, 1974;Greenwald et al, 1984). However, application of this method to insect studies has met with limited success (Peacock, 1976;Komnick & Achenbach, 1979). Indeed, Komnick & Achenbach (1979) reported that the K+-p-NPPase of the rectum of Aeshria cyaneu larvae was inhibited by 2 0 m~ strontium used in the incubation medium and by the fixation process.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of a strontium capture technique for cytochemical localization of potassium‐dependent phosphatase in Malpighian tubules of Locusta

Fogg

Hyde

Anstee

1992

Physiological Entomology

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A strontium capture method, using p‐nitrophenyl phosphate as substrate, was used to determine the subcellular localization of (Na+ + K+)‐ATPase activity in Malpighian tubules of Locusta migratoria L. Ultrastructural studies revealed that (Na+ + K+)‐ATPase activity was restricted to the basolateral plasma membranes with little evidence of activity associated with the apical microvilli. In contrast, alkaline phosphatase activity was specifically associated with the apical cell membrane. Biochemical assays of fixed and non‐fixed tubule homogenates were used to evaluate the p‐nitrophenyl phosphate‐strontium procedure for localization of the phosphatase component of (Na+ + K+)‐ATPase. No significant potassium‐dependent, ouabain‐sensitive p‐nitrophenyl phosphatase activity was demonstrated in homogenates under conditions necessary for the cytochemical procedure, viz fixation, pH 9.0 and the presence of strontium. The significance of the biochemical results are discussed in relation to the validity of such cytochemical techniques for (Na+ + K+)‐ATPase localization.

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Section: Potassium-dependent Phosplzatase In Locusta 139contrasting

confidence: 71%

Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of a strontium capture technique for cytochemical localization of potassium‐dependent phosphatase in Malpighian tubules of Locusta

Fogg

Hyde

Anstee

1992

Physiological Entomology

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Ultrastructure of the ileum of Locusta migratoria

Peacock

1986

Journal of Morphology

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The epithelium of the ileum of Locusta migratoria is composed of a single layer of columnar cells surrounded on the luminal side by cuticle and on the haemocoel side by a circular muscle layer. Where the circular muscles contact the epithelium, longitudinal muscles are present and the epithelium is slightly reduced. Elsewhere, a subepithelial sinus separates the epithelium from the circular muscle layer. The epithelial cells are characterised by extensive infoldings of the apical and basal cell membranes, which in the latter case, produce a maze of interconnecting channels and spaces. Mitochondria are closely associated with both apical and basal infoldings, although the bulk of these organelles is located in the cytoplasm underlying the infoldings of the apical plasma membrane. Vesicles of assorted sizes occur throughout the cytoplasm. They are particularly abundant in the apical region, where they appear to be produced by pinocytosis. Larger vesicles, containing either material of varying electron density or smaller vesicles, occur in the medial and basal regions of the cells. The contents of some of these vesicles have, in section, a lamellar appearance, composed of concentric layers of material. Similar vesicles are present in the basement membrane as well as the cells and connective tissue of the subepithelial sinus. Microtubules and groups of ribosomes commonly occur throughout the cytoplasm, and lipid-like droplets are also present in some of the cells. Ultrastructural features of the epithelial cells are discussed in relation to current knowledge of the function of the ileum.

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Techniques for Studying Na+,K+-ATPase

Anstee¹,

Bowler²

1984

Springer Series in Experimental Entomology

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Distribution of Na+-K+-activated ATPase in the alimentary tract of Locusta migratoria

Cited by 13 publications

References 14 publications

Assessment of a strontium capture technique for cytochemical localization of potassium‐dependent phosphatase in Malpighian tubules of Locusta

Assessment of a strontium capture technique for cytochemical localization of potassium‐dependent phosphatase in Malpighian tubules of Locusta

Ultrastructure of the ileum of Locusta migratoria

Techniques for Studying Na+,K+-ATPase

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