Distribution of Na+,K+-ATPase in rat exocrine pancreas as monitored by K+-NPPase cytochemistry and [3H]-ouabain binding: a plasma membrane protein found primarily to be ductal cell associated.

Madden, Michael E.; Sarras, Michael P.

doi:10.1177/35.12.2824600

Cited by 22 publications

(12 citation statements)

References 15 publications

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“…The Na + /K + ATPase pump is abundantly expressed in the basolateral membrane of the ducts (256, 369, 399, 410). The primary Na + /K + ATPase pump in conjunction with the basolateral K + channels uses and converts the chemical energy in ATP to osmotic energy in the form of the Na + and K + gradients and a negative membrane potential, which fuel fluid and electrolyte secretion by the duct.…”

Section: Ductal Fluid and Hco3− Secretionmentioning

confidence: 99%

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Lee

Ohana

Park

et al. 2012

Physiological Reviews

349

455

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Fluid and HCO3−secretion is a vital function of all epithelia and is required for the survival of the tissue. Aberrant fluid and HCO3−secretion is associated with many epithelial diseases, such as cystic fibrosis, pancreatitis, Sjögren's syndrome, and other epithelial inflammatory and autoimmune diseases. Significant progress has been made over the last 20 years in our understanding of epithelial fluid and HCO3−secretion, in particular by secretory glands. Fluid and HCO3−secretion by secretory glands is a two-step process. Acinar cells secrete isotonic fluid in which the major salt is NaCl. Subsequently, the duct modifies the volume and electrolyte composition of the fluid to absorb the Cl−and secrete HCO3−. The relative volume secreted by acinar and duct cells and modification of electrolyte composition of the secreted fluids varies among secretory glands to meet their physiological functions. In the pancreas, acinar cells secrete a small amount of NaCl-rich fluid, while the duct absorbs the Cl−and secretes HCO3−and the bulk of the fluid in the pancreatic juice. Fluid secretion appears to be driven by active HCO3−secretion. In the salivary glands, acinar cells secrete the bulk of the fluid in the saliva that is driven by active Cl−secretion and contains high concentrations of Na+and Cl−. The salivary glands duct absorbs both the Na+and Cl−and secretes K+and HCO3−. In this review, we focus on the molecular mechanism of fluid and HCO3−secretion by the pancreas and salivary glands, to highlight the similarities of the fundamental mechanisms of acinar and duct cell functions, and to point out the differences to meet gland-specific secretions.

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Section: Ductal Fluid and Hco3− Secretionmentioning

confidence: 99%

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Lee

Ohana

Park

et al. 2012

Physiological Reviews

349

455

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“…The main driving force for ductal HCO 3 -secretion is provided by the Na + ,K + -ATPase, that is strongly expressed in the basolateral membrane of ductal cells (66,97) and is a crucial component to maintain the Na + gradient that allows the accumulation of intracellular HCO 3 -and Cl -by the different transporters: the Na + /H + exchanger, the Na + -HCO 3 -cotransporter and the Na + -K + -2Cl -cotransporter (101).…”

Section: Basolateral Membrane Mechanismsmentioning

confidence: 99%

Pancreatic duct secretion: experimental methods, ion transport mechanisms and regulation

et al. 2008

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The pancreatic ductal tree conveys enzymatic acinar products to the duodenum and secretes the fluid and ionic components of pancreatic juice. The physiology of pancreatic duct cells has been widely studied, but many questions are still unanswered concerning their mechanisms of ionic transport. Differences in the transport mechanisms operating in the ductal epithelium has been described both among different species and in the different regions of the ductal tree. In this review we summarize the methods developed to study pancreatic duct secretion both in vivo and in vitro, the different mechanisms of ionic transport that have been reported to date in the basolateral and luminal membranes of pancreatic ductal cells and the regulation of pancreatic duct secretion by nervous, endocrine and paracrine influences.

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“…[3H]Ouabain binding assays for Na+ , K+-ATPase, a primarily ductal cell-associated enzyme in the pancreas (32,33), demonstrated that MIA PaCa-2 cells have statistically higher levels of this enzyme than detected for rat acini or L cells. Since it was not possible to isolate viable human pancreatic acini or identify freshly isolated human ducts, the specific activity of these preparations could not be compared to MIA PaCa-2 cells.…”

Section: Discussionmentioning

confidence: 99%

“…Naf , Kf-ATPase has been shown to be a basolateral plasma membrane enzyme primarily associated with the ductal epithelium in the pancreas (32,33). As monitored by the cytochemical Kdependent, ouabain-sensitive p-NPPase method of Ernst (20,21), MIA PaCa-2 cells had what appeared to be a basolateral plasma membrane distribution of N a + , K+-ATPase (Fig.…”

Section: E Madden Et Almentioning

confidence: 95%

Comparative Analysis of a Human Pancreatic Undifferentiated Cell Line (MIA PaCa-2) to Acinar and Ductal Cells

Madden¹,

Heaton²,

Huff³

et al. 1989

Pancreas

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Pancreatic ductal cell secretion has not been well characterized due to the difficulty in obtaining sufficient quantities of purified ductal cells. To determine if the MIA PaCa-2 cell line would provide a useful model for in vitro studies of pancreatic ductal cell secretion, the present study was designed to characterize these cells in greater detail. In this investigation, the human pancreatic undifferentiated cell line, MIA PaCa-2, was compared with PANC-1 cells (a human ductal cell line previously characterized), isolated rat and human ducts, acinar cells, and nonpancreatic cell lines. The results indicate that while the morphology of the MIA PaCa-2 cell line is nonpolarized and generally atypical of either ductal or acinar cells, the cell line has retained certain biochemical similarities to ductal cells. Additional morphological studies indicated (a) the presence of intermediate filaments characteristic of epithelial cells, (b) the absence of zymogen granules, and (c) an apparent basolateral plasma membrane localization of Na+ , K+-ATPase. Similar to ductal cells, biochemical analyses indicated (a) the presence of N a + , K+-ATPase based on [3H]-ouabain binding assays, (b) high levels of carbonic anhydrase, (c) low levels of y-glutamyl transpeptidase, (d) nondetectable levels of amylase, and (e) protein composition and protein synthetic patterns comparable to PANC-1 cells. Finally, as with PANC-1 cells and isolated rat and human ducts, the major sulfated secretory product of MIA PaCa-2 cells was a protein with a molecular weight of approximately 660,000 to 1 million. These results suggest that while the MIA PaCa-2 cell line can be used to study pancreatic ductal cell secretion, it possesses less ductal-like characteristics than observed with the PANC-1 cell line.

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Distribution of Na+,K+-ATPase in rat exocrine pancreas as monitored by K+-NPPase cytochemistry and [3H]-ouabain binding: a plasma membrane protein found primarily to be ductal cell associated.

Cited by 22 publications

References 15 publications

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Pancreatic duct secretion: experimental methods, ion transport mechanisms and regulation

Comparative Analysis of a Human Pancreatic Undifferentiated Cell Line (MIA PaCa-2) to Acinar and Ductal Cells

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Distribution of Na+,K+-ATPase in rat exocrine pancreas as monitored by K+-NPPase cytochemistry and [3H]-ouabain binding: a plasma membrane protein found primarily to be ductal cell associated.

Cited by 22 publications

References 15 publications

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO3−Secretion

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO3−Secretion

Pancreatic duct secretion: experimental methods, ion transport mechanisms and regulation

Comparative Analysis of a Human Pancreatic Undifferentiated Cell Line (MIA PaCa-2) to Acinar and Ductal Cells

Contact Info

Product

Resources

About

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion

Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO₃⁻Secretion