Distribution of necrotizing hepatopancreatitis bacterium (NHPB) in cultured white shrimp, Litopenaeus vannamei, from Mexico

Ibarra-Gámez, J C; Galaviz‐Silva, Lucio; Molina-Garza, ZJ

doi:10.7773/cm.v33i1.1044

Cited by 9 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar clinical signs have been reported in cases of infection by the necrotizing hepatopancreatitis bacterium (NHP-B) (3,20) and septic vibriosis (21), where the atrophy of the HP together with the immunological response of the shrimp is associated with necrotic lesions and tubular melanization in the HP tissue. Although certain signs are the same as those associated with diseases and syndromes caused by bacteria of the genus Vibrio and NHP-B found in cultured shrimp around the world (3), a notable difference in the size of the affected shrimp is observed.…”

Section: Discussionmentioning

confidence: 49%

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Soto-Rodríguez

Gómez‐Gil

Lozano‐Olvera

et al. 2015

Appl Environ Microbiol

319

212

View full text Add to dashboard Cite

Moribund shrimp affected by acute hepatopancreatic necrosis disease (AHPND) from farms in northwestern Mexico were sampled for bacteriological and histological analysis. Bacterial isolates were molecularly identified as Vibrio parahaemolyticus by the presence of the tlh gene. The tdh-negative, trh-negative, and tlh-positive V. parahaemolyticus strains were further characterized by repetitive extragenic palindromic element-PCR (rep-PCR), and primers AP1, AP2, AP3, and AP and an ems2 IQ2000 detection kit (GeneReach, Taiwan) were used in the diagnostic tests for AHPND. The V. parahaemolyticus strains were used in immersion challenges with shrimp, and farmed and challenged shrimp presented the same clinical and pathological symptoms: lethargy, empty gut, pale and aqueous hepatopancreas, and expanded chromatophores. Using histological analysis and bacterial density count, three stages of AHNPD (initial, acute, and terminal) were identified in the affected shrimp. The pathognomonic lesions indicating severe desquamation of tubular epithelial cells of the hepatopancreas were observed in both challenged and pond-infected shrimp. The results showed that different V. parahaemolyticus strains have different virulences; some of the less virulent strains do not induce 100% mortality, and mortality rates also rise more slowly than they do for the more virulent strains. The virulence of V. parahaemolyticus strains was dose dependent, where the threshold infective density was 10 4 CFU ml ؊1 ; below that density, no mortality was observed. The AP3 primer set had the best sensitivity and specificity. Field and experimental results showed that the V. parahaemolyticus strain that causes AHPND acts as a primary pathogen for shrimp in Mexico compared with the V. parahaemolyticus strains reported to date.

show abstract

Section: Discussionmentioning

confidence: 49%

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Soto-Rodríguez

Gómez‐Gil

Lozano‐Olvera

et al. 2015

Appl Environ Microbiol

319

212

View full text Add to dashboard Cite

show abstract

“…Las postlarvas, de 7 mg ind -1 de peso promedio, fueron cultivadas en un sistema de recirculación de agua y alimentadas a saciedad (formulación comercial con 40% de proteína cruda), durante de 60 días hasta obtener un peso promedio individual de 4,5 ± 0,4 g. Para verificar el estado de salud antes del experimento, se colectaron 30 individuos al azar a los cuales se realizó un análisis en fresco (Ibarra-Gámez et al, 2006). Adicionalmente, se tomó una muestra de hepatopáncreas para determinar la presencia de bacterias tipo Vibrio mediante la siembra en placas (Gómez-Gil et al, 1998).…”

Section: Materiales Y Métodosunclassified

Efecto del fotoperiodo y la razon camaron:macroalga en la remoción de nitrogeno amoniacal total por Gracilaria vermiculophylla, en cultivo con Litopenaeus vannamei, sin recambio de agua

Sánchez-Romero

Miranda‐Baeza

López‐Elías³

et al. 2013

lajar

View full text Add to dashboard Cite

Efecto del fotoperiodo y la razón camarón:macroalga en la remoción de nitrógeno amoniacal total por Gracilaria vermiculophylla, en cultivo con Litopenaeus vannamei, sin recambio de agua ), N-NO 2 (0.145 ± 0.29 mg L -1 ) y N-NO 3 (3.13 ± 0.73 mg L -1 ) were registered in the biomass rate of 1:8. The highest growth rates of the macroalgae (3.07 ± 1.44% day -1 ) were recorded with 24:00 h photoperiod and biomass ratio 1:8. The highest rate of N incorporation (0.31 ± 0.12 mg N L -1 ) of the macroalgae occurred under the same conditions. Shrimp growth was similar to data reported in traditional recirculation systems. The integration of G. vermiculophylla without water exchange system was able to maintain the TAN and N-NO 2 under levels conditions for the growth of L. vannamei.

show abstract

Assessing the viability of necrotizing hepatopancreatitis bacterium (NHPB) stored at −20°C for use in forced-feeding infection of Penaeus (Litopenaeus) vannamei

et al. 2011

View full text Add to dashboard Cite

Distribution of necrotizing hepatopancreatitis bacterium (NHPB) in cultured white shrimp, Litopenaeus vannamei, from Mexico

Cited by 9 publications

References 11 publications

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico

Efecto del fotoperiodo y la razon camaron:macroalga en la remoción de nitrogeno amoniacal total por Gracilaria vermiculophylla, en cultivo con Litopenaeus vannamei, sin recambio de agua

Assessing the viability of necrotizing hepatopancreatitis bacterium (NHPB) stored at −20°C for use in forced-feeding infection of Penaeus (Litopenaeus) vannamei

Contact Info

Product

Resources

About