Distribution of neuropeptide F in the ventral nerve cord and its possible role on testicular development and germ cell proliferation in the giant freshwater prawn, Macrobrachium rosenbergii

Thongrod, Sirorat; Wanichanon, Chaitip; Sobhon, Prasert

doi:10.1007/s00441-019-02999-8

Cited by 9 publications

(25 citation statements)

References 36 publications

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“…We named neuronal clusters of the ES based on the description of Sandeman et al (1992) and Thongrod et al (2017), while the naming of various parts of the BR, that is, medial protocerebrum (PC), deuto-cerebrum (DC), and tritocerebrum (TC) was based on that described by Sandeman et al (1992) and Thongrod et al (2017). In addition, groups of neurons and neuropils SEG, TG, and AG were named following the description of Tinikul et al (2011) and Thongrod et al (2019).…”

Section: Gross Anatomy and Nomenclature Of The Cnsmentioning

confidence: 99%

“…A polyclonal antiserum against MrNPF was produced previously, and its specificity was also rigorously tested, as previously described (Thongrod et al, 2017(Thongrod et al, , 2019. This primary antiserum was used for subsequent immunolabeling in this study.…”

Section: Antisera Used For Immunolabeling Mrnpfmentioning

confidence: 99%

“…The whole-mount immunohistochemistry protocol for immunolabeling of MrNPF in the BR and VNC tissues was based on Tinikul et al (2011) and Thongrod et al (2017Thongrod et al ( , 2019…”

Section: Whole-mount Immunohistochemistrymentioning

confidence: 99%

“…We characterized sizes of neurons as there was a clear gap between the classes, that is, small-, medium-, and large-sized neurons, whose diameters were approximately 8 ± 3.2, 26 ± 6.4, and 60 ± 7.5 μm, respectively. The classification of these neural sizes was performed based on that described in male M. rosenbergii (Thongrod et al, 2017(Thongrod et al, , 2019.…”

Section: Measurement Of Neuronal Size During the Ovarian Cyclementioning

confidence: 99%

“…In male prawn, M. rosenbergii, MrNPF was detected in several regions of the CNS. This neuropeptide stimulated the proliferation of early male germ cells and increased the gonado-somatic index, suggesting its role in regulating testicular development (Thongrod et al, 2019). In female prawn, M. rosenbergii, MrNPF was detected in the ovaries, and its expression level was more intense at stage III, but slightly declined at stage IV of the ovarian cycle.…”

Section: Introductionmentioning

confidence: 96%

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Comparative neuroanatomical distribution and expression levels of neuropeptide F in the central nervous system of the female freshwater prawn, Macrobrachium rosenbergii, during the ovarian cycle

Tinikul

Kruangkum

Tinikul

et al. 2021

J of Comparative Neurology

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Neuropeptide F (NPF) plays critical roles in controlling the feeding and reproduction of prawns. In the present study, we investigated changes in the expression levels of Macrobrachium rosenbergii neuropeptide F (MrNPF), and its neuroanatomical distribution in eyestalk (ES), brain (BR), subesophageal ganglion (SEG), thoracic ganglia (TG), and abdominal ganglia (AG), during the ovarian cycle of female prawn. By qRT-PCR, the amount of MrNPF transcripts exhibited a gradual increase in the ES, BR, and combined SEG and TG from stages I and II, to reach a maximum level at stage III, and slightly declined at stage IV, respectively. The highest to lowest expression levels were detected in combined SEG and TG, BR, ES, and AG, respectively. MrNPF immunolabeling was observed in several neuronal clusters, associated fibers, and neuropils of these central nervous system (CNS) tissues. MrNPF-ir was more intense in neurons and neuropils of SEG and TG than those found in other parts of the CNS. The number of MrNPFir neurons and intensity of MrNPF-ir were higher in the ES, BR, SEG, and TG at the late stages than those at the early stages of the ovarian cycle, while those in AG exhibited insignificant change. Taken together, there is a correlation between changes in the neuroanatomical distribution of MrNPF and stages of the ovarian cycle, implying that MrNPF may be an important neuropeptide that integrates sensory stimuli, including photo-, chemo-, and gustatory receptions, to control feeding and reproduction, particularly ovarian development, of this female prawn, M. rosenbergii.

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Section: Gross Anatomy and Nomenclature Of The Cnsmentioning

confidence: 99%

Section: Antisera Used For Immunolabeling Mrnpfmentioning

confidence: 99%

“…The whole-mount immunohistochemistry protocol for immunolabeling of MrNPF in the BR and VNC tissues was based on Tinikul et al (2011) and Thongrod et al (2017Thongrod et al ( , 2019…”

Section: Whole-mount Immunohistochemistrymentioning

confidence: 99%

Section: Measurement Of Neuronal Size During the Ovarian Cyclementioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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