Distribution of newly described enterotoxin-like genes in Staphylococcus aureus from food

Bania, Jacek; Dąbrowska, Anna; Bystroń, Jarosław; Korzekwa, Kamila; Chrzanowska, Józefa; Molenda, J.

doi:10.1016/j.ijfoodmicro.2005.10.013

Cited by 89 publications

(61 citation statements)

References 36 publications

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“…The presence of staphylococcal enterotoxin genes sea, seb, sec, sed, see (Johnson et al, 1991), seg, seh, sei, sej, sek, sel, sep, seq (Bania et al, 2006), sem, sen, seo (Jarraud et al, 2002) and seu (Nashev et al, 2007), exfoliative toxins (eta and etb) (Johnson et al, 1991), toxic-shock syndrome toxin (tst) (Booth et al, 2001), α-and β-haemolysins (hla and hlb) (Salasia et al, 2004), protease encoding genes (sspA, sspB and aur) (Karlsson and Arvidson, 2002), lipase encoding gene (geh) (Saïd-Salim et al, 2003), lukE-lukD leukocidin gene (Jarraud et al, 2002), and enterotoxin like protein-1 (set1) (Salasia et al, 2004) were searched by either monoplex or multiplex PCR. The PCR products were resolved in 1.5% (w/v) agarose gel electrophoresis in 1× TAE buffer (0.04 M Tris-acetate and 0.001 M EDTA).…”

Section: Virulence Factor Genesmentioning

confidence: 99%

Virulence properties of methicillin-susceptible Staphylococcus aureus food isolates encoding Panton–Valentine Leukocidin gene

Sudağıdan

Aydın

2010

International Journal of Food Microbiology

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Section: Virulence Factor Genesmentioning

confidence: 99%

Virulence properties of methicillin-susceptible Staphylococcus aureus food isolates encoding Panton–Valentine Leukocidin gene

Sudağıdan

Aydın

2010

International Journal of Food Microbiology

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“…1 and Table 2). The majority of seg and sei distribution studies show that these genes are always detected together in S. aureus because they coexist on a common genetic element, a so-called enterotoxin gene cluster (Jarraud et al, 2001;Rosec and Gigaud, 2002;Stephan et al, 2002;Scherrer et al, 2004;Katsuda et al, 2005;Bania et al, 2006;Boerema et al, 2006;Hata et al, 2006;Kérouanton et al, 2007). It should also be noted that none of our isolates possessed the see, seh, sej, or tst genes, and on 15 out of 20 farms tested, no enterotoxigenic staphylococci were recovered from either mastitic quarter milk or bulk tank milk (Table 2).…”

Section: Pcr Amplification Of Genes Encoding Staphylococcal Enterotoxinsmentioning

confidence: 99%

“…Traditionally, five classical antigenic SE types (SEA, SEB, SEC, SED, and SEE) were recognized. However, in recent years, the existence of new types of SEs, including enterotoxin-like (SEl) toxins (SEG, SEH, SEI, SElJ, SElK, SElL, SElM, SElN, SElO, SElP, SElQ, SElR, SElU, SElU2, and SElV) has been reported and their genes described (Letertre et al, 2003;Lina et al, 2004;Omoe et al, 2004;Jørgensen et al, 2005a;Bania et al, 2006;Boerema et al, 2006;Hata et al, 2006;Thomas et al, 2006). A distantly related protein, toxic shock syndrome toxin 1 (TSST-1), also produced by S. aureus, was the first toxin shown to be involved in the toxic shock syndrome of humans and animals (Akineden et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary

Peles

Wagner

Varga

et al. 2007

International Journal of Food Microbiology

133

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Staphylococcus aureus is a major foodborne pathogen due to its capability to produce a wide range of heat-stable enterotoxins. The primary purpose of this research was to characterize S. aureus isolates recovered from mammary quarter milk of mastitic cows and from bulk tank milk produced on Hungarian dairy farms of different sizes. Macrorestriction analysis of chromosomal DNA from S. aureus isolates was performed using the restriction enzyme SmaI followed by pulsed-field gel electrophoresis (PFGE). The prevalence rates of nine S. aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and of the toxic shock syndrome toxin 1 gene (tst) were determined by multiplex polymerase chain reaction (PCR). The bulk tank milks of 14 out of 20 farms were contaminated with S. aureus at levels of up to 6.0 × 10 3 CFU/ml. Farm size had no significant effect (P N 0.05) on the S. aureus counts in bulk milk. The prevalence rates of penicillin resistance were 88.9% and 20.0% among the S. aureus recovered from mastitic quarter milk and bulk tank milk, respectively. After phenotypic characterization, a total of 59 S. aureus isolates were selected for genotyping. PFGE analysis revealed 22 distinct pulsotypes, including 14 main types and 8 subtypes, at a similarity level of 86%. Only one or two main types were observed on each of the farms tested, indicating a lack of genetic diversity among S. aureus isolates within farms, and there were only two pulsotypes which occurred on more than one farm. The PFGE patterns showed genetic relatedness between the S. aureus strains recovered from quarter milk and bulk milk on two large farms, implying that on farms having a high number of mastitic cows, S. aureus from infected udders may contaminate bulk milk and, subsequently, raw milk products. Sixteen (27.1%) of the S. aureus isolates tested by multiplex PCR were found to be positive for enterotoxin genes, with 15 of them carrying just one gene and one strain carrying two genes (seg and sei). The most commonly detected toxin genes were seb, sea, and sec, whereas none of our isolates possessed the see, seh, sej, or tst genes. On 75% of the dairy farms surveyed, no enterotoxigenic staphylococci were recovered from either mastitic quarter milk or bulk tank milk.

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“…Other enterotoxins, marked J-V, are called the putative SEls (Staphylococcal Enterotoxin-like superantigens) because they showed no emetic properties in animal studies (Lina et al 2004, Collery et al 2008, Podkowik et al 2013. Another classification of enterotoxins involves their division into classical enterotoxins (five antigenically different types of SEA, SEB, SEC, SED, SEE) and new enterotoxins (SEG-SElV) (Bania et al 2006, Park et al 2011. Genes encoding staphylococcal enterotoxins may be located on plasmids, bacteriophages, transposons, or pathogenicity islands (PIs) and are transferred by horizontal gene transfer.…”

Section: Introductionmentioning

confidence: 99%

Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

Piechota

Kot

Zdunek

et al. 2014

Polish Journal of Veterinary Sciences

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The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with-and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes.The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes.The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.

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Distribution of newly described enterotoxin-like genes in Staphylococcus aureus from food

Cited by 89 publications

References 36 publications

Virulence properties of methicillin-susceptible Staphylococcus aureus food isolates encoding Panton–Valentine Leukocidin gene

Virulence properties of methicillin-susceptible Staphylococcus aureus food isolates encoding Panton–Valentine Leukocidin gene

Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary

Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

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