Distribution of NO-Induced cGMP-Like Immunoreactive Neurones in the Abdominal Nervous System of the Crayfish, Procambarus clarkii

Aonuma, Hitoshi

doi:10.2108/zsj.19.969

Cited by 12 publications

(11 citation statements)

References 45 publications

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“…This is shown pharmacologically in preparations from the abdominal nervous system of the crayfish Pacifastacus leniusculus (Aonuma and Newland, 2002). Moreover, cGMP IR is elevated after NO stimulation of certain cells in the cardiac ganglion of the crab Cancer productus, and in the abdominal nervous system of the crayfish Procambarus clarcii (Aonuma, 2002). In contrast, NO does not affect luminescence through the cGMP pathway in fireflies and hatchetfish, two species in which NO has been demonstrated to be involved in the regulation of light production.…”

Section: Cgmpmentioning

confidence: 95%

Serotonin and nitric oxide interaction in the control of bioluminescence in northern krill, Meganyctiphanes norvegica (M. Sars)

Krönström

Dupont²,

Mallefet

et al. 2007

Journal of Experimental Biology

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Section: Cgmpmentioning

confidence: 95%

Serotonin and nitric oxide interaction in the control of bioluminescence in northern krill, Meganyctiphanes norvegica (M. Sars)

Krönström

Dupont²,

Mallefet

et al. 2007

Journal of Experimental Biology

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“…8). Such a suggestion has been alluded to in our previous studies, although we had no direct evidence that the increased levels of NO were acting via sGC (Aonuma andNewland, 2001, 2002). By applying L-arginine and ODQ simultaneously to the terminal ganglion, the L-arginine-induced increase in reflex response was blocked by ODQ, implying that NO exerts its modulatory effects through sGC and cGMP, which provides the important evidence that has been lacking until now.…”

Section: Endogenous No Levels Contribute To Reflex Responsesmentioning

confidence: 69%

“…and Newland, 2001), the depression of spiking local interneurons, and the down-regulation of presynaptic inhibition , all by virtue of the patterns of connections they make with these classes of interneurons. It is worth noting, however, that the cGMP staining pattern observed by Aonuma (2002) is not the exception to the rule, because Bicker et al (1996) showed that in the olfactory system of locusts NO also acts on intrinsic interneurons. Clearly there is much still to understand as to where NO is mediating these effects within the terminal ganglion and the precise mechanisms of its action, or indeed where it is acting.…”

Section: No Action In Local Circuitsmentioning

confidence: 94%

“…In crayfish, physiological studies have also pointed to a presynaptic site of action of NO on mechanosensory neurons, because NO had no effect on the membrane potential or input impedances of ascending interneurons (Aonuma and Newland, 2001) or on spiking local interneurons (Aonuma and Newland, 2002). Given the apparent similarity with the synaptic organization of the local circuits of insects, it is somewhat surprising that a recent study by Aonuma (2002) described a strikingly different pattern of NO-induced cGMP staining in the terminal abdominal ganglion of crayfish, in which specific local and intersegmental interneurons are described as being potential targets for NO. This suggests that a proposed model for NO action, in which activity-dependent NO acts on the terminals of neighboring sensory neurons (Ott et al, 2000(Ott et al, , 2001, is unlikely in the crayfish terminal ganglion.…”

Section: No Action In Local Circuitsmentioning

confidence: 99%

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Nitric oxide modulates local reflexes of the tailfan of the crayfish

Araki¹,

Schuppe²,

Fujimoto³

et al. 2004

J. Neurobiol.

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Electrical stimulation of sensory neurons that innervate receptors on the tailfan of crayfish evokes a reflex response of motor neurons that produce movements of the blades of the tailfan, the uropods. We analyzed the modulatory effects of nitric oxide (NO) on the spike frequency of the reflex response. Bath application of L-arginine and SNAP, which elevate endogenous and exogenous NO levels, increased the frequency of the evoked response, whereas the application of L-NAME and PTIO, which reduce NO levels, decreased the frequency of the response. To determine through what pathway and target NO exerted these effects we bath applied ODQ, an inhibitor of soluble guanylyl cyclase (sGC), which decreased the frequency of response, and 8-br-cGMP, which increased the spike frequency of response. To provide further evidence that NO acts via sGC, we elevated NO levels with L-arginine while simultaneously inhibiting sGC with ODQ. This application reduced the response to control levels, indicating that NO in the terminal ganglion of crayfish acts via sGC to modulate cGMP levels, which in turn regulate the responses of the uropod motor neurons.

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“…These results indicate that NO activates sGC and promotes the production of cGMP. Aonuma (Aonuma, 2002) showed the distribution of NO-induced cGMP-like imunoreactive neurones in the abdominal nervous system. Some axons projecting from nerve 1 of the abdominal ganglia are immunopositive (H. Aonuma, personal communication).…”

Section: Research Articlementioning

confidence: 99%

Nitric oxide modulates a swimmeret beating rhythm in the crayfish

Mita

Yoshida

Nagayama

2014

Journal of Experimental Biology

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The modulatory effects of nitric oxide (NO) and cAMP on the rhythmic beating activity of the swimmeret motor neurones in the crayfish were examined. Swimmerets are paired appendages located on the ventral side of each abdominal segment that show rhythmic beating activity during forward swimming, postural righting behaviour and egg ventilation in gravid females. In isolated abdominal nerve cord preparations, swimmeret motor neurones are usually silent or show a continuous low-frequency spiking activity. Application of carbachol, a cholinergic agonist, elicited rhythmic bursts of motor neurone spikes. The co-application of L-arginine, the substrate for NO synthesis with carbachol increased the burst frequency of the motor neurones. The co-application of the NO donor SNAP with carbachol also increased the burst frequency of the motor neurones. By contrast, co-application of a NOS inhibitor, L-NAME, with carbachol decreased beating frequency of the motor neurones. These results indicate that NO may act as a neuromodulator to facilitate swimmeret beating activity. The facilitatory effect of L-arginine was cancelled by co-application of the soluble guanylate cyclase (sGC) inhibitor ODQ suggesting that NO acts by activating sGC to promote the production of cGMP. Application of L-arginine alone or membrane-permeable cGMP analogue 8-Br-cGMP alone did not elicit rhythmic activity of motor neurones, but co-application of 8-Br-cGMP with carbachol increased bursting frequency of the motor neurones. Furthermore, application of the membrane-permeable cAMP analogue CPT-cAMP alone produced rhythmic bursting of swimmeret motor neurones, and the bursting frequency elicited by CPT-cAMP was increased by coapplication with L-arginine. Co-application of the adenylate cyclase inhibitor SQ22536 ceased rhythmic bursts of motor neurone spikes elicited by carbachol. These results suggest that a cAMP system enables the rhythmic bursts of motor neurone spikes and that a NO-cGMP signaling pathway increases cAMP activity to facilitate swimmeret beating.

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Distribution of NO-Induced cGMP-Like Immunoreactive Neurones in the Abdominal Nervous System of the Crayfish, Procambarus clarkii

Cited by 12 publications

References 45 publications

Serotonin and nitric oxide interaction in the control of bioluminescence in northern krill, Meganyctiphanes norvegica (M. Sars)

Serotonin and nitric oxide interaction in the control of bioluminescence in northern krill, Meganyctiphanes norvegica (M. Sars)

Nitric oxide modulates local reflexes of the tailfan of the crayfish

Nitric oxide modulates a swimmeret beating rhythm in the crayfish

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