Distribution of Oligomannosyl Side Chains in the Cell Wall Mannan of Pichia pastoris Purified by Benanomicin A

Anomeric Proton and Carbon (H1-C1) NMR Chemical Shifts of Antigenic Mannans Obtained from Pathogenic Yeast <i>Candida tropicalis</i>

“…Summarizing these analysis results, the overall structure of C. tropicalis NBRC 0199 and 1400 strain mannans can be proposed as shown in Figure 2. In the previous study [18], it was shown to be suitable to analyze the comblike yeast mannan composed of α-1,2-, β-1,2and α-1,6-linked mannose residues by two-dimensional H-C COSY. In this report, we have shown that this procedure is also useful in the structural analysis of similar mannan composed of α-1,2-, α-1,3-, β-1,2-, and α-1,6-linked mannose residues.…”

“…13 C-NMR spectrum (internal CD 3 OD, 49.00 ppm) was measured with the same spectrometer on solutions (15 -25 mg sample/0.7mL) in D 2 O at 55˚C [17]. Two-dimensional H-C COSY was also recorded under the same conditions as for the 1 H-and 13 C-NMR spectra in accordance with previous description [18].…”