Distribution of PAF-acetylhydrolase activity in human plasma low-density lipoprotein subfractions

Karabina, Sonia-Athena P.; Liapikos, Theodores A.; Grekas, Georgios; Goudevenos, John; Tselepis, Alexandros D.

doi:10.1016/0005-2760(94)90219-4

Cited by 83 publications

(65 citation statements)

References 23 publications

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“…4,5 The present work demonstrates that LDL-borne PAF-AH is preferentially bound to LDL(Ϫ). Increased PAF-AH activity associated with LDL(Ϫ) in NL subjects concurs with reports of preferential distribution of PAF-AH in dense LDL subfractions, 17 because LDL(Ϫ) from NL subjects has been reported to be more abundant in dense subfractions. 16,18 However, LDL(Ϫ) from FH patients is abundant in light subfractions 16 ; hence, the observation that PAF-AH was also preferentially carried in LDL(Ϫ) from FH suggests that its association depends on lipoprotein characteristics other than density or size.…”

Section: Discussionsupporting

confidence: 88%

Platelet-Activating Factor Acetylhydrolase Is Mainly Associated With Electronegative Low-Density Lipoprotein Subfraction

et al. 2003

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Background-Electronegative LDL [LDL(Ϫ)], a modified subfraction of LDL present in plasma, induces the release of interleukin-8 and monocyte chemotactic protein-1 from cultured endothelial cells. Methods and Results-We demonstrate that platelet-activating factor acetylhydrolase (PAF-AH) is mainly associated with LDL(Ϫ). LDL(Ϫ) had 5-fold higher PAF-AH activity than the nonelectronegative LDL subfraction [LDL(ϩ)] in both normolipemic and familial hypercholesterolemic subjects. Western blot analysis after SDS-PAGE confirmed these results, because a single band of 44 kDa corresponding to PAF-AH appeared in LDL(Ϫ) but not in LDL(ϩ).Nondenaturing polyacrylamide gradient gel electrophoresis demonstrated that PAF-AH was bound to LDL(Ϫ) regardless of LDL size. In accordance with the above findings, nonesterified fatty acids, a cleavage product of PAF-AH, were increased in LDL(Ϫ) compared with LDL(ϩ). Conclusions-The

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Section: Discussionsupporting

confidence: 88%

Platelet-Activating Factor Acetylhydrolase Is Mainly Associated With Electronegative Low-Density Lipoprotein Subfraction

et al. 2003

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“…This is consistent with the preferential distribution of Lp-PLA 2 in the sdLDL subfraction observed in our gradient ultracentrifugation experiments (16,25,26,29 ). However, serum triglyceride concentrations are a better predictor of sdLDL; in fact, an increased plasma triglyceride concentration is biochemically a prerequisite for the formation of sdLDL particles.…”

Section: Discussionsupporting

confidence: 88%

“…Using a gradient ultracentrifugation technique, we have previously shown that in plasma of either normolipidemic volunteers (16,25 ) or patients with various types of dyslipidemia (26,27 ), the majority of the LDLassociated Lp-PLA 2 activity was bound to sdLDL particles. However, it remains to be established whether the plasma enzyme activity or mass could be a marker of these atherogenic sdLDL particles in plasma.…”

mentioning

confidence: 99%

Lipoprotein-Associated Phospholipase A2 Activity Is a Marker of Small, Dense LDL Particles in Human Plasma

Gazi¹,

Lourida

Filippatos³

et al. 2005

Clinical Chemistry

159

101

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Background:Recent clinical studies showed that lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ) is a predictor for incident atherosclerotic disease. We have previously shown that among the LDL subfractions, Lp-PLA 2 activity is preferentially associated with the atherogenic small, dense (sdLDL) particles in vitro. We investigated whether Lp-PLA 2 could be a marker of sdLDL in human plasma. Methods: One hundred and seventy-six individuals participated in the study. LDL subclass analysis was performed by polyacrylamide gel electrophoresis. Lp-PLA 2 activity and mass were determined in total plasma and in apolipoprotein B-depleted plasma (HDL-Lp-PLA 2 ). Non-HDL-Lp-PLA 2 activity and mass were calculated by subtracting the HDL-Lp-PLA 2 from total plasma Lp-PLA 2 . Results: On the basis of the LDL subclass analysis, participants were categorized into phenotype A and non-A (total cholesterol mass of the sdLDL subfractions <0.155 and >0.155 mmol/L, respectively). Unlike total plasma Lp-PLA 2 mass, total plasma Lp-PLA 2 activity and non-HDL-Lp-PLA 2 activity and mass were significantly higher in persons with phenotype non-A compared with persons with phenotype A, whereas HDLLp-PLA 2 activity and mass were lower in persons with phenotype non-A compared with phenotype A. Total plasma activity and non-HDL-Lp-PLA 2 activity and mass, but not Lp-PLA 2 mass, were correlated with sdLDL-cholesterol mass, proportion, and mean LDL

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“…Notably, the LDL-cholesterol or the cholesterol levels in thalassemic patients are not correlated with enzyme mass or activity, in contrast to expectations based on the results from most studies including those from our group, published to date ( 18,26 ). As previously shown, the majority of the LDL-associated Lp-PLA 2 is bound to atherogenic sdLDL particles in both normolipidemic and dyslipidemic populations ( 18,22,26,29 ). Our results revealed that both patient groups are characterized by a predominance of sdLDL particles, a fi nding that is consistent with their elevated fasting triglyc- Similar to the total plasma Lp-PLA 2 , the HDL-Lp-PLA 2 also is elevated in both patient groups, despite the low HDL-cholesterol levels, and it also could be primarily attributed to the enhanced enzyme secretion from monocytes/macrophages.…”

Section: Discussioncontrasting

confidence: 73%

Plasma levels of lipoprotein-associated phospholipase A2 are increased in patients with β-thalassemia

Tselepis

Hahalis

Tellis

et al. 2010

Journal of Lipid Research

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Distribution of PAF-acetylhydrolase activity in human plasma low-density lipoprotein subfractions

Cited by 83 publications

References 23 publications

Platelet-Activating Factor Acetylhydrolase Is Mainly Associated With Electronegative Low-Density Lipoprotein Subfraction

Platelet-Activating Factor Acetylhydrolase Is Mainly Associated With Electronegative Low-Density Lipoprotein Subfraction

Lipoprotein-Associated Phospholipase A2 Activity Is a Marker of Small, Dense LDL Particles in Human Plasma

Plasma levels of lipoprotein-associated phospholipase A2 are increased in patients with β-thalassemia

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