Distribution of Pathogenicity Islands Among Uropathogenic Escherichia coli Isolates From Patients With Urinary Tract Infections

Asadi, Ehsan; Mohammadzadeh, Mohammad; Niakan, Mohammad

doi:10.34172/ijep.2020.09

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…The highest and lowest PAIs were PAI IV536 (81.8%) and PAI J196 (6.4%) among UPEC isolates. This result is consistent with other investigations in Iran (38), China (24), the Czech Republic (39), and Sweden (40). Among UPEC strains known as high PAI, PAI IV536 has been described as the most common PAI.has been described as the most common PAI.…”

Section: Discussionsupporting

confidence: 92%

Serogroup and Pathogenicity Island Marker Distributions Among Uropathogenic Escherichia coli Isolates in Rasht, Iran

Shamami

Anvari

Pourmoshtagh

et al. 2023

Jundishapur J Microbiol

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Background: Urinary tract infections (UTIs) are among the most prevalent infections in hospitals and communities worldwide. Objectives: Due to the medical importance of UTIs caused by uropathogenic Escherichia coli (UPEC), this study aimed to investigate pathogenicity island (PAI) markers, O-antigen serogroups, and resistance to antibiotic agents associated with UPEC isolates obtained from hospitalized patients in Rasht city hospitals. Methods: A total of 110 urine samples were taken from patients with UTI referred to selected hospitals in Rasht, Iran. The double-disk synergy test (DDST) was used to detect the isolate’s ability to produce extended-spectrum β-lactamase (ESBL). Using particular primers, eight PAIs were detected (ie, PAI I536, PAI II536, PAI III536, PAI IV536, PAI ICFT073, PAI IICFT073, PAI IJ96, and PAI IIJ96). Results: According to the antibiotic susceptibility pattern, a high level of antibiotic resistance was observed against nalidixic acid (81.8%) and co-trimoxazole (78.2%), while the most effective agent was amikacin (85.5%). Double-disk synergy test revealed that the incidence of ESBL-positive strains was 62.7% (69/110). Of the 110 UPEC isolates, 106 (96.4%) carried at least one of the investigated PAI markers. UPEC isolates with PAI IV536 (81.8%) had the highest prevalence, and PAI J196 (6.4%) had the lowest PAI marker. The most predominant serogroup O was O25 (36.4%), followed by O16 (17.3%), while the O4 and O7 serogroups (0.9%) were the lowest serogroups among UPEC isolates. Conclusions: The characterization of our strain revealed the co-occurrence of PAI and serogroups, confirming the importance of antibiotic resistance among the distinct serogroups and PAI markers. Our results have potential application for epidemiological studies and designing UTI treatment strategies against UTIs caused by UPEC.

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Section: Discussionsupporting

confidence: 92%

Serogroup and Pathogenicity Island Marker Distributions Among Uropathogenic Escherichia coli Isolates in Rasht, Iran

Shamami

Anvari

Pourmoshtagh

et al. 2023

Jundishapur J Microbiol

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Anticancer effects of cell-free culture supernatant of Escherichia coli in bladder cancer cell line: New insight into the regulation of inflammation

Mehmandar-Oskuie,

Tohidfar,

Hajikhani

et al. 2023

Gene

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Distribution of Pathogenicity Islands among Escherichia coli Strains from Mansoura Universty Hospitals

Mowafy,

Degla,

Edrees

2024

Preprint

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This study was carried out to illustrate the pathogenic E. coli, that had pathogenicity islands (PAIs) containing genes with many virulence factors (VFs). Thirty (2.9%) E. coli strains were isolated from adults (18 female and 12 male) from 1020 positive cultures of all pathogens isolated from Mansoura University Hospitals through one year from January 2022 to January 2023. The age of patients ranged from 20–50 in 56.6% and above 50 years old in 43.3%. E. coli were isolated from urine 18 (60%), stool 6 (20%) and Bronchoalveolar lavage (BAL) 6 (20%). Among 30 E. coli isolates 25 were positive PAIs examined using PCR, 11 (36.6%) had PAI ˂ 4, 14 (46.6%) had PAI ≥ 4 and no PAIs were detected in 5 isolates. Among E. coli isolates, PAI IJ96 had the highest prevalence were found in 15 E. coli strains (60%), the second was PAI III536 found in 14 (56%), PAI IICF073 13 (52%), PAI II536 2 (8%) both PAI I536 and PAI ICFT073 were 4 (16%), while the lowest prevalence was PAI IV536 1 (4%) and PAI II J96 (0%) was not detected in this study. In conclusion, PAI IJ96 and PAI III536 were the most frequent pathogenicity islands in this study and E.coli became more virulent and dangerous in the human health, especially infected with E. coli strains contains PAI IJ96.

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Distribution of Pathogenicity Islands Among Uropathogenic Escherichia coli Isolates From Patients With Urinary Tract Infections

Cited by 3 publications

References 21 publications

Serogroup and Pathogenicity Island Marker Distributions Among Uropathogenic Escherichia coli Isolates in Rasht, Iran

Serogroup and Pathogenicity Island Marker Distributions Among Uropathogenic Escherichia coli Isolates in Rasht, Iran

Anticancer effects of cell-free culture supernatant of Escherichia coli in bladder cancer cell line: New insight into the regulation of inflammation

Distribution of Pathogenicity Islands among Escherichia coli Strains from Mansoura Universty Hospitals

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