Distribution of Porphyromonas gingivalis fimA Genotypes in Chronic Apical Periodontitis Associated with Symptoms

Wang, Qian; Zhou, Xuedong; Zheng, Qianqian; Wang, Yao; Tang, Lu; Huang, Dingming

doi:10.1016/j.joen.2010.08.018

Cited by 24 publications

(23 citation statements)

References 32 publications

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“…These studies have shown that, in severe marginal periodontitis, type II is most frequently detected, followed by type IV (2,24). Similarly, in apical periodontitis, type II P. gingivalis shows the highest appearance ratio in symptomatic focus and type I is isolated from asymptomatic focus (41). The present study was designed to investigate the effect of antibiotics on adherent cells and multilayer biofilms.…”

Section: Discussionmentioning

confidence: 94%

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Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

Maezono

Noiri

Asahi

et al. 2011

Antimicrob Agents Chemother

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Antibiotic resistance of biofilm-grown bacteria contributes to chronic infections, such as marginal and periapical periodontitis, which are strongly associated with Porphyromonas gingivalis. Concurrent azithromycin (AZM) administration and mechanical debridement improve the clinical parameters of periodontal tissue in situ. We examined the in vitro efficacy of AZM against P. gingivalis biofilms. The susceptibilities of adherent P. gingivalis strains 381, HW24D1, 6/26, and W83 to AZM, erythromycin (ERY), ampicillin (AMP), ofloxacin (OFX), and gentamicin (GEN) were investigated using a static model. The optical densities of adherent P. gingivalis cells were significantly decreased by using AZM and ERY at sub-MIC levels compared with those of the controls in all the strains tested, except for the effect of ERY on strain W83. AMP and OFX inhibited P. gingivalis adherent cells at levels over their MICs, and GEN showed no inhibition in the static model. The effects of AZM and ERY against biofilm cells were investigated using a flow cell model. The ATP levels of P. gingivalis biofilms were significantly decreased by AZM at concentrations below the sub-MICs; however, ERY was not effective for inhibition of P. gingivalis biofilm cells at their sub-MICs. Furthermore, decreased density of P. gingivalis biofilms was observed three-dimensionally with sub-MIC AZM, using confocal laser scanning microscopy. These findings suggest that AZM is effective against P. gingivalis biofilms at sub-MIC levels and could have future clinical application for oral biofilm infections, such as chronic marginal and periapical periodontitis.

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Section: Discussionmentioning

confidence: 94%

“…A lot of research has been carried out to study the relationship between the stage of progression of periodontitis and differences in genotypes (2,24,35,41). These studies have shown that, in severe marginal periodontitis, type II is most frequently detected, followed by type IV (2,24).…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

Maezono

Noiri

Asahi

et al. 2011

Antimicrob Agents Chemother

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“…The microbiological profiles of persistent endodontic infections have been analyzed by a variety of methods including conventional culture systems [4,[10][11][12], polymerase chain reaction [13], reverse-capture checkerboard analysis [14], and pyrosequencing [15]. Evidence from these studies has shown that the microbial flora from persistent endodontic infections differs markedly from that found in untreated necrotic dental pulp, quantitatively as well as qualitatively.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Imamura

Tatehara

Takebe

et al. 2014

International Journal of Photoenergy

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The purpose of this study is to evaluate the usefulness of 405 nm monochromatic laser irradiation as an alternative management for prevention and/or treatment of endodontic infections. A monochromatic laser-emitting device equipped with a 405-nm laser diode was developed. Using this device, the effect of 405 nm laser irradiation on the growth of Porphyromonas gingivalis, Prevotella intermedia, Enterococcus faecalis, and Candida albicans, which are microorganisms associated with persistent endodontic infections, was evaluated by viable colony counting. As a result, the irradiation with a 405 nm laser had a significant bactericidal/fungicidal effect on P. gingivalis, P. intermedia, and C. albicans, whereas the growth of E. faecalis was not affected by the irradiation. The inhibition rate in P. gingivalis and P. intermedia was ∼60% and ∼80%, respectively, following irradiation at 0.2 W for 300 sec. The inhibition rate in C. albicans was ∼90% following irradiation at 0.2 W for 1200 sec. These results indicate that 405 nm monochromatic laser irradiation exerts a bactericidal/fungicidal effect on these microorganisms. The present study clearly demonstrates that 405 nm laser irradiation is a promising alternative management strategy for prevention and/or treatment of endodontic infections.

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“…During FFPE preparation, the samples were soaked in formaldehyde. This treat- (10)(11)(12). Furthermore, to confirm the specificity of PCR-LAMP, IHC was performed using the same 110 FFPE samples.…”

Section: Discussionmentioning

confidence: 99%

“…Culture has been used to detect P. gingivalis in root canals during endodontic treatment (prevalence, 6.7-15%) (9,10) but is poorly suited for clinical diagnosis because it requires time-consuming microbiological culture and laboratory confirmation and has a low detection rate. Molecular biological techniques including PCR have recently been developed, and these methods have much higher detection rates for P. gingivalis (28-43%) (11,12).…”

Section: Introductionmentioning

confidence: 99%

Loop-mediated isothermal amplification combined with PCR and immunohistochemistry for detecting <i>Porphyromonas gingivalis</i> in periapical periodontitis

Kitano

Mikami

Igarashi

et al. 2016

Journal of Oral Science

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Porphyromonas gingivalis is important in the development of marginal periodontitis. However, the precise role and localization of P. gingivalis in chronic periapical periodontitis remain unclear. Thus, methods that can detect P. gingivalis in formalin-fixed and paraffin-embedded (FFPE) tissue samples are needed. We assessed a technique combining loopmediated isothermal amplification (LAMP) with PCR (PCR-LAMP) for detection of P. gingivalis, using 110 FFPE tissue samples of chronic apical periodontitis. PCR-LAMP specifically detected P. gingivalis with high sensitivity in FFPE tissue samples, and the sensitivity of the technique was higher than that of PCR or LAMP alone. The results of immunohistochemistry (IHC) confirmed the specificity of PCR-LAMP. IHC showed that P. gingivalis was localized in a granular layer of chronic apical periodontitis, a region that correlated with the localization of macrophages. This is the first study to describe the localization of P. gingivalis in human periapical periodontitis. In conclusion, PCR-LAMP was an effective tool for detecting P. gingivalis in periapical periodontitis. In addition, IHC results improve our understanding of the role of P. gingivalis in the progression of periapical periodontitis. (J Oral Sci 58, 163-169, 2016)

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Distribution of Porphyromonas gingivalis fimA Genotypes in Chronic Apical Periodontitis Associated with Symptoms

Cited by 24 publications

References 32 publications

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

Antibiofilm Effects of Azithromycin and Erythromycin on Porphyromonas gingivalis

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Loop-mediated isothermal amplification combined with PCR and immunohistochemistry for detecting <i>Porphyromonas gingivalis</i> in periapical periodontitis

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