Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lomé, Togo

Salah, Fortune Djimabi; Soubeïga, Serge Théophile; Ouattara, Abdoul Karim; Sadji, Adodo Yao; Mètuor-Dabiré, Amana; Obiri‐Yeboah, Dorcas; Banla-Kere, Abiba; Karou, Simplice Damintoti; Simporé, Jacques

doi:10.1186/s13756-019-0552-0

Cited by 110 publications

(102 citation statements)

References 32 publications

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“…Our study showed that all the E. coli isolates (5/5) were ESBL producers in agreement with 95.60% reported in Togo [41]. The presence of ESBL-producing bacteria in hospitals is a major challenge that affects both developed and developing countries [26].…”

Section: Discussionsupporting

confidence: 88%

Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso

Dembélé

Konaté²,

Traoré

et al. 2020

BMC Pediatr

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Background The emergence and spread of multidrug-resistant gram-negative bacteria (MDR) has become a major public health concern worldwide. This resistance is caused by enzymes-mediated genes (i.e., extended spectrum beta-lactamases) that are common in certain Enterobacterioceae species. However, the distribution of these genes is poorly documented in Burkina Faso. This study aims to determine the prevalence and distribution of the resistant genes coding for broad spectrum beta-lactamases and quinolones in rural Burkina Faso. Methods Multiplex PCR assays were carried out to detect ESBL-encoding genes, including blaOXA, blaTEM, blaCTX-M, blaSHV. The assays also assessed the presence of quinolone resistance gene namely qnrA, qnrB and qnrS in the quinolone-resistance DEC and Salmonella strains. Results The Extended-Spectrum Beta-Lactamases (ESBL) resistance phenotype was reported in all the E. coli isolates (5/5). Cross-resistance phenotype to quinolones (CRQ) was shown by one Salmonella strain (1/9) and three E. coli (3/5). Cross-resistance phenotypes to fluoroquinolones (CRFQ) were harboured by one Salmonella (1/9) and carbapenemase phenotypes were detected in two E. coli strains (2/5). Whilst the blaOXA genes were detected in 100% (5/5) of E. coli isolates and in 33.33% (3/9) Salmonella isolates. One strain of E. coli (1/5) harbored the blaCTX−M gene and the qnrB gene simultaneously. Conclusions This study identified β-lactam (bla) and quinolone resistance (qnr) genes in multidrug-resistant E. coli and Salmonella spp. in rural Burkina Faso. Our finding which highlighted the enterobacteriaceae strains resistance to β-lactams and quinolones are of high interest for adequate management of antimicrobial resistant genes outbreak in Burkina Faso.

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Section: Discussionsupporting

confidence: 88%

Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso

Dembélé

Konaté²,

Traoré

et al. 2020

BMC Pediatr

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“…We also reported the prevalence of plasmid-mediated quinolone resistance in Salmonella and E. coli. Only a single isolate of E. coli (20%) was positive for the qnrB gene which is lower than 67.21% reported in Togo [41] and higher than 3.17% reported in Niger [52]. No Salmonella strain was positive for the qnr genes in the present study.…”

Section: Discussioncontrasting

confidence: 75%

Extended spectrum beta-lactamase and ﬂuoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso.

Dembélé

Konaté²,

Traoré

et al. 2020

Preprint

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Background: The emergence and spread of multidrug-resistant gram-negative bacteria (MDR) has become a major public health concern worldwide. This resistance is caused by enzymes-mediated genes (i.e., extended spectrum beta-lactamases) that are common in certain Enterobacterioceae species. However, the distribution of these genes is poorly documented in Burkina Faso. This study aims to determine the prevalence and distribution of the resistant genes coding for broad spectrum beta-lactamases and quinolones in rural Burkina Faso.Methods: Multiplex PCR assays were carried out to detect ESBL-encoding genes, including blaOXA, blaTEM, blaCTX-M, blaSHV. The assays also assessed the presence of quinolone resistance gene namely qnrA, qnrB and qnrS in the quinolone-resistance DEC and Salmonella strains.Results: The Extended-Spectrum Beta-Lactamases (ESBL) resistance phenotype was reported in all the E. coli isolates (5/5). Cross-resistance phenotype to quinolones (CRQ) was shown by one Salmonella strain (1/9) and three E. coli (3/5). Cross-resistance phenotypes to fluoroquinolones (CRFQ) were harboured by one Salmonella (1/9) and carbapenemase phenotypes were detected in two E. coli strains (2/5). Whilst the blaOXA genes were detected in 100% (5/5) of E. coli isolates and in 33.33% (3/9) Salmonella isolates. One strain of E. coli (1/5) harbored the blaCTX-M gene and the qnrB gene simultaneously.Conclusions: This study identified β-lactam (bla) and quinolone resistance (qnr) genes in multidrug-resistant E. coli and Salmonella spp. in rural Burkina Faso. Our finding which highlighted the enterobacteriaceae strains resistance to β-lactams and quinolones are of high interest for adequate management of antimicrobial resistant genes outbreak in Burkina Faso.

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“…In another study, a low frequency for qnr gene isolation was also reported among E. coli by Pereira et al [40] in Brazil. Furthermore, currently, resistance against quinolones and qnr genes has increased in many parts of the world including places like in Iran among E. coli [41], China among Shigella [42], Togo among Klebsiella spp., [43]; Salmonella isolates [44], and in Hungary among E. coli, Klebsiella, Citrobacter, Enterobacter, and Serratia species [45,46].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of qnr Genes among Multidrug Resistance Staphylococcus aureus from Clinical Isolates

Abdu

Mirabeau

2019

JAMMR

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Objectives: Staphylococcus aureus (S. aureus) is regarded as an important aetiological agent of various human infections. Fluoroquinolones are routinely used in the chemotherapeutic management of these infections; nonetheless, in recent years, a growing rate of resistance to these drugs has been reported worldwide. The aims of this study were to isolate and discover the prevalence of plasmid-mediated (qnrA, qnrB, and qnrS) genes among the quinolone-resistant clinical S. aureus isolates in Bayelsa State, Nigeria. Methods: A total of 25 (31.25%) clinical isolates of S. aureus were collected from hospitalized patients. The bacterial isolates were identified through standard laboratory protocols and further confirmed using the API Staph system (bioMérieux, France) test strips. The antimicrobial susceptibility and minimum inhibitory concentration (MIC) were determined by the standard disk diffusion and serial dilutions methods respectively. Polymerase chain reaction (PCR) was used for detecting qnrA, qnrB, and qnrS genes. Results: Of the 25 S. aureus isolates, 19(76.00%) were resistant to ampicillin-cloxacillin, while 14 (56.00%) each were resistant to norfloxacin and Amoxicillin, 13 (52.00%) each to gentamicin and erythromycin, 11 (44.00%) were resistant to streptomycin, rifampicin and ciprofloxacin, respectively. The resistance pattern among the isolates to chloramphenicol and levofloxacin were 10 (40.00%) and 7 (28.00%) respectively. All the eleven ciprofloxacin resistant were high-level (1000 µg/mL) resistance isolates and only one (9.00%) of these isolates was positive for the qnrB gene. Conclusion: The study results were indicative of the presence of low frequency of qnr genes among the clinical isolates of S. aureus in Yenagoa, indicating that other mechanisms are employed in resisting to these fluoroquinolones. This, however, emphasizes the need for establishing discreet policies associated with infection-control measures in hospital settings.

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Distribution of quinolone resistance gene (qnr) in ESBL-producing Escherichia coli and Klebsiella spp. in Lomé, Togo

Cited by 110 publications

References 32 publications

Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso

Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso

Extended spectrum beta-lactamase and ﬂuoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso.

Prevalence of qnr Genes among Multidrug Resistance Staphylococcus aureus from Clinical Isolates

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