Distribution of radiation sensitivities for human tumor cells of specific histological types: Comparison of in vitro to in vivo data

Radiation sterilisation of cultured human brain tumour cells for clinical immune tumour therapy

“…Other previously documented studies of the intrinsic cellular radiation sensitivity of noninfected human glioblastoma cell lines have mostly used irradiation at absorbed dose levels below 12 Gy (Malaise et al, 1986;Taghian et al, 1992;Allam et al, 1993;Taghian et al, 1995;Wilkins et al, 1996;Budach et al, 1997;Raaphorst et al 1999). The first study of five human glioma cell lines resulted in the mean lethal dose D 0 ¼ 1.470.4 Gy (Malaise et al, 1986).…”

“…The first study of five human glioma cell lines resulted in the mean lethal dose D 0 ¼ 1.470.4 Gy (Malaise et al, 1986). A second study of 10 human glioblastoma cell lines in vitro resulted in the mean lethal dose D 0 ¼ 1.670.5 (Taghian et al, 1992(Taghian et al, , 1995 and a third study of 11 human glioblastoma cell lines in vitro resulted in a mean inactivation dose of D 0 ¼ 2.070.6 (Allam et al, 1993).…”

“…Previous studies of the intrinsic cellular radiation sensitivity of noninfected human glioblastoma cell lines have used low radiation dose levels in order to investigate the shoulder of the survival curves (Malaise et al, 1986;Taghian et al, 1992). In the present study, however, we examine the radiation survival characteristics at much higher radiation dose levels.…”

Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia

“…The first category included embryonic tumours and lymphomas, the second squamous cell and adenocarcinomas and the third gliomas, sarcomas and melanomas. More recently, it has been shown that this observed clinical variation in radiation response could be explained in part by differences in in vitro sensitivity of cell lines (Malaise et al, 1986). For cervical cancer (West et al, 1993), glioma (Ramsay et al, 1992) and head and neck cancers (Brock et al, 1990) there is also a wide range of in vitro radiosensitivity within each histological group and that this may correlate with clinical response (West et al, 1993).…”

The RBE of Fast Neutrons for in Vitro Inactivation of Human Tumour Cells Determined by the Ratio of Mean Inactivation Doses

“…A convenient single parameter that describes overall sensitivity is the mean inactivation dose (MID) (10,11). Its use carries many advantages: 1) it represents the radiosensitivity of the cell population as a whole; 2) it predicts radiosensitivity among histological groups better than any other parameter (12); 3) it has a low coefficient of variation (12)(13)(14); 4) it has units of dose (Gy); 5) it is strongly influenced by survival at clinically relevant doses; and 6) its use is recommended by the ICRU (15 extreme values of sensitivity such as in ataxia telangiectasia cells, which may distort the distribution (16).…”