Distribution of RAPD Markers on a Linkage Map of Barley

BackgroundHigher plants possess a large multigene family encoding secreted class III peroxidase (Prx) proteins. Peroxidases appear to be associated with plant disease resistance based on observations of induction during disease challenge and the presence or absence of isozymes in resistant vs susceptible varieties. Despite these associations, there is no evidence that allelic variation of peroxidases directly determines levels of disease resistance.Methodology/Principal FindingsThe current study introduces a new strategy called Prx-Profiling. We showed that with this strategy a large number of peroxidase genes can be mapped on the barley genome. In order to obtain an estimate of the total number of Prx clusters we followed a re-sampling procedure, which indicated that the barley genome contains about 40 peroxidase gene clusters. We examined the association between the Prxs mapped and the QTLs for resistance of barley to homologous and heterologous rusts, and to the barley powdery mildew fungus. We report that 61% of the QTLs for partial resistance to P. hordei, 61% of the QTLs for resistance to B. graminis and 47% of the QTLs for non-host resistance to other Puccinia species co-localize with Prx based markers.Conclusions/SignificanceWe conclude that Prx-Profiling was effective in finding the genetic location of Prx genes on the barley genome. The finding that QTLs for basal resistance to rusts and powdery mildew fungi tend to co-locate with Prx clusters provides a base for exploring the functional role of Prx-related genes in determining natural differences in levels of basal resistance.

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“…Prx2 [13], Prx7 [37], Prx4 [38], or from Prx -like EST sequences. They were recently located on transcript maps of barley [13], [39], [40].…”

Section: Discussionmentioning

confidence: 99%

Peroxidase Profiling Reveals Genetic Linkage between Peroxidase Gene Clusters and Basal Host and Non-Host Resistance to Rusts and Mildew in Barley

González

Marcel²,

Kohutova³

et al. 2010

PLoS ONE

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“…When a homozygous crop like barley is studied, dominant markers can be applied in the same manner as codominant markers (RFLP, isozymes) (CLARK and LANIGAN 1993). Disadvantages of RAPDs also include problems with reproducibility (SKROCH and NIENHUIS 1995) and clustering to certain chromosomal areas (GIESE et al 1994). In our case the unreproducible RAPD bands were omitted.…”

Section: Dna Markersmentioning

confidence: 83%

Genetic Diversity Among Finnish Six-Rowed Barley Cultivars Based on Cpedigree Information and DNA Markers

Manninen

Nissilä

2004

Hereditas

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Manninen, 0. and Nissila, E. Genetic diversity among Finnish six-rowed barley cultivars based on pedigree information and DNA markers. -Hereditas 126: 87-93. Lund, Sweden.Genetic diversity among Finnish six-rowed spring barleys was studied by both pedigree analysis and random amplified polymorphic DNA (RAPD) markers. 26 six-rowed barley varieties have been released by Finnish plant breeders, 22 of which are based on controlled crosses. These 22 varieties originate from 14 ancestors, of which 7 (Olli, Asplund, Vega, Gull, Hanna, Maskin, OAC21) are principal ancestors with a coancestry r > 0.05. Contribution of the principal ancestors to the pedigrees of the varieties has not changed significantly during the 60 years of barley breeding in Finland. Jaccard's similarity indices, based on 54 polymorphic RAPD markers, were calculated for a set of modern, ancestral and introgression lines and varieties. Jaccard's indices varied from 0. I54 to 0.935, and similarities between related accessions were significantly higher (t-test, P i 0.01) than between the unrelated ones. Correlation between kinship coefficients based on pedigrees and Jaccard's indices were weak, and Jaccard's indices varied widely for accessions without a known pedigree. The genetic similarity within the old varieties is comparable to that of the modern varieties.

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“…The SSrDNA locus was mapped using the same technique and mapping population as described in GIESE et al (1994).…”

Section: Rflp-mappingmentioning

confidence: 99%

Towards an Integration of the Physical and the Genetic Chromosome Maps of Barley by in Situ Hybridization

Pedersen¹,

Giese²,

Linde‐Laursen³

2004

Hereditas

Self Cite

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I. 1995. Towards an integration of the physical and the genetic chromosome maps of barley by in situ hybridization -Hereditas 123: 77-88. Lund, Sweden. ISSN 0018-0661.The relationship between physical and genetic distances on the barley chromosomes was studied by fluorescence in situ hybridization (FISH). Physical mapping of low-and single copy genes by FISH includes the protein Zx gene on chromosome 4 (41), the a-amylase, high PI, genes on chromosome 6 (61), the early-methionine-labelled polypeptide gene(s) on chromosome 5 (II), and the leaf thionin genes on chromosome 6 (61). Two interstitial sites of the telomere-associated sequence, the HvRT-family, were mapped by FISH on chromosome 3 (31), and hybridization sites of histone 4 and ubiquitin genes were detected on chromosomes 1 (71) and 5 (lI), respectively. In addition, a 5SrDNA locus was mapped genetically on chromosome I. The study provides seven new anchor sites for integrating the genetic and physical chromosome maps and allows estimation of the relationship between physical and genetic distances to 1-2 Mb/cM for the distal 40 % of the chromosome arms. All chromosome arms studied showed a strongly reduced level of recombination in their inner halves. These results are discussed in relation to the feasibility of map-based cloning in barley and other cereal species.

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Distribution of RAPD Markers on a Linkage Map of Barley

Cited by 47 publications

References 23 publications

Peroxidase Profiling Reveals Genetic Linkage between Peroxidase Gene Clusters and Basal Host and Non-Host Resistance to Rusts and Mildew in Barley

Peroxidase Profiling Reveals Genetic Linkage between Peroxidase Gene Clusters and Basal Host and Non-Host Resistance to Rusts and Mildew in Barley

Genetic Diversity Among Finnish Six-Rowed Barley Cultivars Based on Cpedigree Information and DNA Markers

Towards an Integration of the Physical and the Genetic Chromosome Maps of Barley by in Situ Hybridization

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