Distribution of Salmonella in swine herds in Québec

Letellier, Ann; Messier, Serge; Paré, Julie; Ménard, J; Quessy, Sylvain

doi:10.1016/s0378-1135(99)00049-8

Cited by 99 publications

(70 citation statements)

References 15 publications

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“…Copenhagen, and the group G isolates as serotype Typhimurium. The prevalences of particular serotypes can also be difficult to compare among studies due to regional differences, seasonal changes, and alternate microbiological methodologies; however, several studies performed in North America and Europe reported on the predominance of serotype Derby and/or serotype Typhimurium in swine (4,7,9,13,16,20), results similar to those presented here. Of particular interest is that 35% of the Salmonella isolates recovered in this study were PFGE group F isolates, identified as serotype Typhimurium subsp.…”

Section: Discussionsupporting

confidence: 84%

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Use of Pulsed-Field Gel Electrophoresis To Characterize the Heterogeneity and Clonality ofSalmonellaIsolates Obtained from the Carcasses and Feces of Swine at Slaughter

Wonderling

Pearce

Wallace

et al. 2003

Appl Environ Microbiol

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Salmonella enterica isolates were recovered from swine at a collaborating processing plant over a 2-month period in the spring of 2000. In the present study, molecular subtyping by pulsed-field gel electrophoresis (PFGE) was performed on the 581 confirmed Salmonella isolates from the 84 Salmonella-positive samples obtained from the previous study. A total of 32 different PFGE pulsotypes were observed visually, and a BioNumerics software analysis clustered those pulsotypes into 12 PFGE groups. The B, F, and G groups predominated throughout the sampling period and were isolated from 39, 22, and 13% of the swine, respectively. In addition, multiple isolates were obtained from 67 of the 84 Salmonella-positive samples, and subtyping revealed multiple PFGE profiles in 35 of these 67 (62%) samples. Both carcass and fecal isolates of Salmonella were recovered from 13 swine, resulting in "matched" samples. Molecular typing of the 252 isolates recovered from the matched samples revealed that 7 (54%) of the 13 carcasses were contaminated with Salmonella pulsotypes that were not isolated from the feces of the same animal. Conversely, from 6 of the 13 (46%) matched animals, Salmonella clonal types were isolated from the feces that were not isolated from the carcass of the same animal. These data establish that each lot of swine introduces new contaminants into the plant environment and that swine feces from one animal can contaminate many carcasses. In addition, these results indicate that the examination of multiple Salmonella isolates from positive samples is necessary to determine the variety of potential contaminants of swine carcasses during slaughter and processing.

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Section: Discussionsupporting

confidence: 84%

“…The isolation of serotype Typhimurium subsp. Copenhagen from swine at much lower frequencies has been reported in several studies (7,9,13,16,20). Serovar Typhimurium subsp.…”

Section: Discussionmentioning

confidence: 81%

Use of Pulsed-Field Gel Electrophoresis To Characterize the Heterogeneity and Clonality ofSalmonellaIsolates Obtained from the Carcasses and Feces of Swine at Slaughter

Wonderling

Pearce

Wallace

et al. 2003

Appl Environ Microbiol

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“…S. Derby has been associated with pigs ( 11 , 12 ). Pork and other swine products were not sampled in this report, but a frequent occurrence of the serovar was observed among unspecified other food products, which could include pork.…”

Section: Discussionmentioning

confidence: 99%

SalmonellaSerovars from Humans and Other Sources in Thailand, 1993–2002

Bangtrakulnonth¹,

Pornreongwong²,

Pulsrikarn³

et al. 2004

Emerg. Infect. Dis.

183

131

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“…For example, salmonellae are frequently found in association with animal manure (11,18,23,24), which is often applied as fertilizer to soil prior to vegetable production, thereby introducing a potential risk of contamination of vegetables grown in the manure-amended soil. This threat is evident from the fact that during recent years, the consumption of raw vegetables has been related to food-borne outbreaks (4,7,9,14,20,21,27).…”

mentioning

confidence: 99%

Comparison of Methods of Extracting Salmonella enterica Serovar Enteritidis DNA from Environmental Substrates and Quantification of Organisms by Using a General Internal Procedural Control

Klerks

Bruggen

Zijlstra

et al. 2006

Appl Environ Microbiol

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This paper compares five commercially available DNA extraction methods with respect to DNA extraction efficiency of Salmonella enterica serovar Enteritidis from soil, manure, and compost and uses an Escherichia coli strain harboring a plasmid expressing green fluorescent protein as a general internal procedural control. Inclusion of this general internal procedural control permitted more accurate quantification of extraction and amplification of S. enterica serovar Enteritidis in these samples and reduced the possibility of false negatives. With this protocol it was found that the optimal extraction method differed for soil (Mobio soil DNA extraction kit), manure (Bio101 soil DNA extraction kit), and compost (Mobio fecal DNA extraction kit). With each method, as little as 1.2 ؋ 10 3 to 1.8 ؋ 10 3 CFU of added serovar Enteritidis per 100 mg of substrate could be detected by direct DNA extraction and subsequent S. enterica-specific TaqMan PCR. After bacterial enrichment, as little as 1 CFU/100 mg of original substrate was detected. Finally, the study presents a more accurate molecular analysis for quantification of serovar Enteritidis initially present in soil or manure using DNA extraction and TaqMan PCR.

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Distribution of Salmonella in swine herds in Québec

Cited by 99 publications

References 15 publications

Use of Pulsed-Field Gel Electrophoresis To Characterize the Heterogeneity and Clonality ofSalmonellaIsolates Obtained from the Carcasses and Feces of Swine at Slaughter

Use of Pulsed-Field Gel Electrophoresis To Characterize the Heterogeneity and Clonality ofSalmonellaIsolates Obtained from the Carcasses and Feces of Swine at Slaughter

SalmonellaSerovars from Humans and Other Sources in Thailand, 1993–2002

Comparison of Methods of Extracting Salmonella enterica Serovar Enteritidis DNA from Environmental Substrates and Quantification of Organisms by Using a General Internal Procedural Control

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