Distribution of selected elements in atherosclerotic plaques of apoE/LDLR‐double knockout mice assessed by synchrotron radiation‐induced micro‐XRF spectrometry

Abstract

Apolipoprotein E and LDL receptor double-knockout (apoE/LDLR

Determination of the elemental composition of tissues of the cardiovascular system by atomic spectrometry, mass spectrometry and X-ray spectrometry methods

“…Such methods as ED XRF, electron probe microanalysis and nuclear microprobe analysis can be used for the measurement of intracellular concentra tions of chemical elements in different types of cells [118]; however, their application is limited by rela [152][153][154]. For the last two decades, the development of the third generation sources of synchrotron radiation as well as X ray optics enables the application of X ray radiation of extremely high brightness for the analysis of space distribution of chemical elements with high resolution and low detection limits in biological sam ples [49,[155][156][157][158][159]. The size of the produced micro beam can be 1-3 µm in diameter and is achieved by using mono and polycapillary X ray focusing lenses and half lenses.…”

“…In particular, the researchers applied the method of microXRF involving synchrotron radiation for the study of the elemental composition and distribution of the elements in myocardium tissue and smooth mus cles of aorta obtained from rats for the study of athero sclerosis [49]. These experiments were carried out using DORIS III storage ring (HASYLAB, Hamburg, Germany).…”

“…A large volume of the investigations of the role of microelements in the activity of the cardiovascular system actually implies the determination of the con centrations of chemical elements in biological liquids in humans [74][75][76][77][78] and directly in heart and vessel tis sue [16,35,43,49,56,72,73,79]; in some cases hair is used for the study [80,81]. JOURNAL OF ANALYTICAL CHEMISTRY Vol.…”

“…Cu 2+ ion induces the formation of lipid peroxides both in the presence and in the absence of hydrogen peroxide. On the other hand, copper ion is a component of Cu/Zn SOD which is involved in scavenging reactive oxygen species [43,49].…”

Chemical Composition of Atherosclerotic Plaques οf apoE/LDLR-Double Knockout Mice by Synchrotron Radiation FTIR Microspectroscopy

“…Biological effects of new antiatherosclerotic drugs and diets have been studied on this model [2,3]. Moreover, X-ray based synchrotron radiation microprobes were successfully applied to characterize elemental composition of atherosclerotic lesions in apoE/LDLR −/− mice [4]. Nutritional habits strongly influence the development * corresponding author; e-mail: wojciech.kwiatek@ifj.edu.pl of atherosclerosis.…”