Distribution of Some Possible Intermediates of
the Haem Biosynthesis

Sg, Smith; Rv, Belcher

doi:10.1159/000459300

Cited by 6 publications

(2 citation statements)

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“…In spite of the logic of this possibility, it is probably not the case here since tricarboxylic porphyrins, whose formation and detection implies that coproporphyrinogen III oxidase activity is not impaired (Cavaleiro et al 1973, Smith & Belcher 1974, were detected in aluminum-treated RS-2 cultures ( Figure 2B). Furthermore, their detection in aluminumtreated cultures (as in the anoxic cultures; Figure 2C) might indicate that under these conditions this enzyme's activity is greater than in the control cultures.…”

Section: The Effect Of Aluminum On Intracellular Heme Concentrations mentioning

confidence: 89%

“…Tricarboxylic porphyrins, which were implicated as the intermediate forms of the enzymatic oxidative decaboxylation of coproporphyrinogen III protoporphyrinogen IX by coproporphyrinogen III oxidase (Figure 3, Cavaleiro et al 1973, Smith & Belcher 1974, were detected in elevated levels in supernatants of aluminum treated and anoxic cultures but not in control cultures (Figure 2). Protoporphyrin IX was almost undetected in any of the treatments (Figure 2).…”

Section: Interference Of Aluminum With Porphyrin Biosynthesismentioning

confidence: 99%

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Evidence for the interference of aluminum with bacterial porphyrin biosynthesis

et al. 1994

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Aluminum (0.74 mM) was found to retard bacterial growth, and enhance porphyrin formation and excretion in Arthrobacter aurescens RS-2. Coproporphyrin III was shown to be the main porphyrin excreted by aluminum-exposed A. aurescens RS-2 cultures and by RS-2 cultures grown under anoxic conditions. Synthesis and excretion of porphyrins in A. aurescens RS-2 increased in a dose-dependent manner when the bacteria were exposed to increasing aluminum concentrations. Incubation of A. aurescens RS-2 with delta-aminolevulinic acid (delta-ALA, 1.2 mM) brought about the intense formation and excretion of porphyrins by the cells, in the presence or absence of aluminum. delta-ALA slightly enhanced the toxicity of aluminum towards RS-2 bacteria. Furthermore, the intracellular concentration of heme was reduced by 63.9 +/- 8.67% in aluminum-exposed RS-2 bacteria when compared with control cultures. The results are discussed in light of the recent finding concerning aluminum toxicity and porphyrin biosynthesis in microorganisms.

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Section: The Effect Of Aluminum On Intracellular Heme Concentrations mentioning

confidence: 89%

Section: Interference Of Aluminum With Porphyrin Biosynthesismentioning

confidence: 99%