Distribution of ³H‐fucose in the transseptal ligament of the mouse

Abstract

Johnson RB.: Distribution of^H-fucose in the transseptal ligament of the mouse. J Periodont Res 1988; 23: 363-369. The present study demonstrated 'H-fucose distribution in three regions of the transseptal hgament; the middle, mesial (adjaeent to the first molar tooth) and distal (adjacent to the second molar tooth) thirds; of 6-week-and 6-month-old Swiss mice. Incorporation of the isotope was rapid and maximal peaks occurred at 3 hours in most regions of 6-month and at 12 h in most regions of 6-wk animals: highest grain counts were over the middle third of the ligament of 6-wk animals (p< 0.001). During incorporation, mean grain counts were significantly different as a function of age (p<0.001), region (p<0.01) and time (p<0.001) and there was a significant interaction between region and time (p< 0.005). Grain removal occurred at a slower rate: there were significant differences in mean grain counts as a function of age (p<0.001), region (p<0.001) and time (p<0.00!) and a significant interaction between time and region (p<0.001) and between time, region and age (p<0.001). The highest rate of grain removal was in the middle third of the ligament of 6-wk animals. Half-hfe of labeled glycoproteins was shortest in the ligament of 6-wk animals. The study suggested regional differences in metabolism of fucose-containing glycoproteins of the transseptai ligament: highest turnover rates in the 1) middle third, and 2) young animals.