Distribution of the Level of Antibody to AIDS‐Associated Virus (LAV) in Sera from AIDS or AIDS Related Complex and Japanese Hemophiliacs Infected with AIDS‐Associated Virus

Abstract

The level of antibody to AIDS-associated virus (LAV) in sera from patients with AIDS or AIDS related diseases (AIDS related complex; ARC) and Japanese hemophiliacs was studied using indirect immunofluorescence.Titer of anti-LAV antibody in sera from 89 patients with AIDS or ARC ranged between 10 and 40,960 (median : 1,280) and that of 83 Japanese hemophiliacs ranged from 20 to 20,480(median : 640). The distribution of the level of anti-LAV antibody in hemophiliacs was similar to that of patients with AIDS or ARC, and no correlation between the titer of antibody and the presence of symptoms of AIDS was observed.Our results suggested that hemophiliacs in Japan might have been infected with live LAV rather than been immunized with inactivated LAV by injection of factor VIII or IX concentrate, and more hemophiliacs in Japan might show symptoms of AIDS in the future as in the United States.

Prevalence and clinical implications of occult hepatitis B viral infection in hemophilia patients in Japan

“…The genotypes of viruses such as HCV or GBV-C in the Japanese hemophilia patients are foreign and not domestic genotypes [Kinoshita et al, 1993;Toyoda et al, 1998]. This is because, in this population, transmission of these viruses has been by imported blood products, as well as HIV transmission in this population [Tsuchie et al, 1985]. In contrast, only the domestic HBV genotype was found in the Japanese hemophilia patients.…”

“…The high prevalence of infection with HIV [Tsuchie et al, 1985;Kroner et al, 1994], HCV [ Makris et al, 1990;Troisi et al, 1993], and GBV-C [Hanley et al, 1998;Toyoda et al, 1998] has been reported in many studies. The status of serological markers on HBV infection has also been reported [Kumar et al, 1993;Goedert et al, 2002].…”

“…High prevalence of infection with hepatitis C virus (HCV) [Makris et al, 1990;Troisi et al, 1993], human immunodeficiency virus (HIV) [Tsuchie et al, 1985;Kroner et al, 1994], and GB virus C (GBV-C) [Hanley et al, 1998;Toyoda et al, 1998] has been reported. A few studies have also been carried out on hepatitis B virus (HBV) infection in this population by serological evaluation [Kumar et al, 1993;Goedert et al, 2002].…”

Replication of Human Immunodeficiency Virus in Two T‐Cell Lines and Their Application as Antigens for Immunofluorescence

“…To confirm the results of the anti-HIV assay, we employed IF using TALL-1 infected with HIV as the antigen (20,21), since only 30% of TALL-1 expressed HIV antigens and the rest of the cells could serve as the control. This study also suggests that TALL-1 infected with HIV were suitable as the antigen for IF, because gp120, p65, p55, p24, and p17 could be detected by WB when the percentage of IF-positive cells was at its peak.…”