Distribution of the molecular forms of Anopheles gambiae and pyrethroid knock down resistance gene in Nigeria

Awolola, T. S.; Oyewole, Isaac O.; Amajoh, C. N.; Idowu, Emmanuel Taiwo; Ajayi, M.B.; Oduola, A. M. J.; Manafa, Ogenna; Ibrahim, Kolade Tahiru; Koekemoer, Lizette L.; Coetzee, Maureen

doi:10.1016/j.actatropica.2005.06.002

Cited by 69 publications

(71 citation statements)

References 18 publications

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“…The comparably low KDT 50 values recorded in our study suggest the presence of other mechanisms of resistance in the populations. Although, we did not perform any studies on the frequency of this mutation in our study localities, reports previously published from Oyo State indicate that the kdr mutation is present at a frequency of 0% (Djouaka et al, 2007: Djouaka et al, 2008 to 48.1% (Awolola et al, 2005;Awolola et al, 2007). However, the relatively low frequency of this mutation in certain areas of Oyo State is suggestive of the involvement of metabolic mechanisms of resistance (Brooke et al, 1999;Awolola et al, 2003;Oduola et al, 2010).…”

Section: Discussionmentioning

confidence: 90%

“…The ratio of sympatry between Anopheles colluzzi and Anopheles gambiae s.s. in our study was 95:5 at Oyo and 50:50 at Eruwa. Previous studies in Oyo State have reported the ratio as 39:61 (Awolola et al, 2005); 35:65 (Awolola et al, 2007) and 100:0 (Djouaka et al, 2007;2008). The varying frequencies of these species in different studies around the same region for samples that were collected around the same period of the year may be attributed to changes in species dynamics which is hence recommended for further studies.…”

Section: Discussionmentioning

confidence: 99%

“…Our study revealed that Anopheles coluzzi, Anopheles arabiensis and Anopheles gambiae s.s. are the three species present in the six localities surveyed. Studies had indicated that these three species are widely distributed throughout Nigeria at varying frequencies across geopolitical zones (Onyabe and Conn, 2001;Onyabe et al, 2003;Awolola et al, 2005;PMI, 2015). This scenario also played out between our sample sites.…”

Section: Discussionmentioning

confidence: 99%

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High Level of DDT Resistance and Reduced Susceptibility to Deltamethrin in Anopheles gambiae, Anopheles coluzzi, and Anopheles arabiensis from Urban Communities in Oyo State, South-West Nigeria

Adeogun

Popoola

Oduola

et al. 2017

jmr

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Selection of vector populations capable of withstanding doses of insecticides is a major threat facing insecticide based vector control interventions. In view of the rapidly evolving insecticide resistance in the primary vector, Anopheles, there is need to provide information on the status of insecticide resistance in Oyo State where Long Lasting Insecticide Treated Nets (LLIN) is the only intervention that has been deployed over the years. Morphologically identified Anopheles mosquito larva were collected from six localities (Ojoo, Iwo road, Bodija, Oluyole, Oyo town and Eruwa) in Oyo State. The larval samples were reared to adults and exposed to DDT (4%) and Deltamethrin (0.05%), according to WHO criteria. Knockdown was recorded every ten minutes and mortality scored at 24 hrs post exposure. The KDT 50 and KDT 95 were determined using probit regression analysis and species identification was by adult morphology and Polymerase Chain Reaction techniques. A total of 1,800 female anopheline mosquitoes were exposed to DDT and Deltamethrin insecticides. All the Anopheles mosquito populations from the six localities were resistant to DDT with 13-84% mortality. The anopheline mosquito populations from Iwo road and Oyo town were susceptible to Deltamethrin, while those from Ojoo and Eruwa showed reduced susceptibility to the insecticide. However,mosquito populations from Oluyole and Bodija were resistant to Deltamethrin. The knock down values for DDT and Deltamethrin across all sites was comparatively high indicative of some degree of selection within the populations. Of the 1,764 positively identified anophelines, 639 (36.2%), 1,034 (58.6%) and 91 (5.2%) were Anopheles coluzzi, Anopheles arabiensis and Anopheles gambiae s.s. respectively. This data indicates the widespread resistance of Anopheles coluzzi, Anopheles arabiensis and Anopheles gambiae s.s. to Dichlotrichloroethane (DDT) in Oyo State. It also showed an ongoing selection against pyrethroid which poses a major threat to the ongoing LLIN intervention in the State.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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High Level of DDT Resistance and Reduced Susceptibility to Deltamethrin in Anopheles gambiae, Anopheles coluzzi, and Anopheles arabiensis from Urban Communities in Oyo State, South-West Nigeria

Adeogun

Popoola

Oduola

et al. 2017

jmr

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“…gambiae s.l. to DDT and pyrethroids (PYR) is especially conferred in West Africa by mutation of the sodium channel target site, the L1014F kdr (Chandre et al, 1999;Diabaté et al, 2002;Awolola et al, 2005;Nguessan et al, 2007). Burkina Faso is composed of three agro-climatic zones and the use of insecticides to control agricultural and human health pests varies considerably in the different zones particularly as the main cotton cropping areas are found in the south west of the country.…”

Section: Introductionmentioning

confidence: 99%

Trends in Insecticide Resistance in Natural Populations of Malaria Vectors in Burkina Faso, West Africa: 10 Years’ Surveys

Dabiré¹,

Diabaté²,

Namountougou³

et al. 2012

Insecticides - Pest Engineering

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“…gambiae s.l. namely in Côte d'Ivoire, Burkina Faso, Benin, Ghana and Nigeria (Yawson et al, 2004;Awolola et al, 2005;Corbel et al, 2007;Djogbé nou et al, 2007;Oduola et al, 2010;Yadouleton et al, 2011;Dabire et al, 2012;Koffi et al, 2012), as well as in Central Africa particularly in countries such as Equatorial Guinea and Cameroon by Nwane et al (2009), Etang et al (2006 and Reimer et al (2005).…”

Section: Discussionmentioning

confidence: 99%

Status and Genes Involved in Insecticide Resistance in Anopheles gambiae Sibling Species in (Togo) West Africa

Accrobessy¹,

Olé²,

Dorkenoo³

et al. 2017

jmr

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Malaria vector control relies on mosquito susceptibility to insecticides. Nowadays, the phenomenon of mosquitoes' resistance to insecticides is growing wider and wider, including all chemical families of insecticides. In order to update data on the insecticides susceptibility, the species' distribution and genes involved in insecticide resistance in Anopheles in the capital of Togo, we tested local strains of An. gambiae s.l. from three study sites in Lomé , with five insecticides namely DDT, Permethrin, Deltamethrin, Bendiocarb, and Fenitrothion. The tests had been performed with the WHO kits from 2013 to 2015. The results of the tests showed mortality rates of 16.0% with 4% DDT, 28.0% with 0.75% Permethrin, 33.0% with 0.05% Deltamethin, 44.0% with 0.1% Bendiocarb and 98.8% with 1% Fenitrothion. The major malaria vectors were shown, across all sites, to be resistant to all of the classes of insecticides used in the experiments except Fenitrothion. PCR analyses for the species' identification showed, proportions of 81% of An. gambiae s.s. and 19% of An. coluzzii in the city. For the Kdr gene, PCR analyses showed proportions of 57.94% RR, 33.33% RS and 8.73% of SS, revealing a high prevalence of kdr resistance in the Anopheles population in Lomé . However, analyses showed mosquitos without Ace1R gene. The multiple resistance to various insecticides is a major concern for the control of malaria and other vector-borne diseases in Lomé , as well as in Togo.

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Distribution of the molecular forms of Anopheles gambiae and pyrethroid knock down resistance gene in Nigeria

Cited by 69 publications

References 18 publications

High Level of DDT Resistance and Reduced Susceptibility to Deltamethrin in <i>Anopheles gambiae</i>, <i>Anopheles coluzzi</i>, and <i>Anopheles arabiensis</i> from Urban Communities in Oyo State, South-West Nigeria

High Level of DDT Resistance and Reduced Susceptibility to Deltamethrin in <i>Anopheles gambiae</i>, <i>Anopheles coluzzi</i>, and <i>Anopheles arabiensis</i> from Urban Communities in Oyo State, South-West Nigeria

Trends in Insecticide Resistance in Natural Populations of Malaria Vectors in Burkina Faso, West Africa: 10 Years’ Surveys

Status and Genes Involved in Insecticide Resistance in <i>Anopheles gambiae</i> Sibling Species in (Togo) West Africa

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