Distribution of the organellar Ca2+ transport ATPase SERCA2 isoforms in the cat brain

Baba-Aı̈ssa, Fawzia; Raeymaekers, Luc; Wuytack, Frank; Greef, Christine De; Missiaen, Ludwig; Casteels, Rik

doi:10.1016/s0006-8993(96)01037-2

Cited by 42 publications

(34 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1) and tiger salamander (Krizaj et al, 2004) retinas. These results indicate that SERCA2 isoform is ubiquitous in the vertebrate retina, similar to its expression in the CNS (Baba-Aissa et al, 1996;Wu et al, 1995). Ca 2+ release from intracellular stores has been suggested to play an important role in stabilization of contacts between inner retinal cells in dendritic stratification that occurs during retinal development (Lohmann et al, 2002).…”

Section: Light Microscopic Localization Of Sercas In the Mammalian Rementioning

confidence: 56%

“…Immunoblotting and immunofluorescence studies have shown that SERCA2 represents the main SERCA isoform in most neuronal tissues (Wu et al, 1995;Wuytack et al, 2002). Consistent with the prominent SERCA2 expression in the brain (Baba-Aissa et al, 1996;Wu et al, 1995), intense staining was observed in mouse, rat, squirrel and macaque retinas. These results suggest that, SERCA2 is expressed in the mammalian retina across many species.…”

Section: Serca2 Is Prominently Expressed In the Mammalian Retinamentioning

confidence: 62%

“…SERCA2 is the most widespread of all SERCA pumps and is phylogenetically the oldest (Baba-Aissa et al, 1996;Wuytack et al, 2002). Immunoblotting and immunofluorescence studies have shown that SERCA2 represents the main SERCA isoform in most neuronal tissues (Wu et al, 1995;Wuytack et al, 2002).…”

Section: Serca2 Is Prominently Expressed In the Mammalian Retinamentioning

confidence: 99%

See 2 more Smart Citations

Serca isoform expression in the mammalian retina

Križaj¹

2005

Experimental Eye Research

View full text Add to dashboard Cite

The sarcoplasmic-endoplasmic reticulum calcium ATPase (SERCA) is a key intracellular calcium transporter, which regulates cellular calcium concentration [Ca 2+ ] by transporting Ca 2+ ions from the cytosol into the endoplasmic reticulum. SERCA-mediated Ca 2+ sequestration controls proper folding of newly synthesized proteins within the ER as well as the timing and spatial patterning of depolarization-evoked Ca 2+ responses in the cytoplasm. We studied expression and distribution of all three SERCA isoforms in the mouse retina using isoform-specific antibodies. No immunostaining was observed with the SERCA1 antibody. SERCA2 was expressed in photoreceptor inner segments, amacrine and ganglion cells of the mouse retina. Similar SERCA2 localization was observed in adult rat, macaque and ground squirrel retinas. Analysis of distribution of SERCA2 immunofluorescence in the developing mouse retina revealed prominent SERCA2 signals throughout postnatal development. The antibody raised against the SERCA3 isoform labeled inner segments of photoreceptors and cell bodies in the inner nuclear layer of the mouse retina. The SERCA3 signal was detected in the inner plexiform layer of the early postnatal retina, but moved by P10 to the outer retina where it was concentrated in outer segments of cones. These results indicate that SERCA2 represents the dominant SERCA isoform in the mammalian retina. SERCA3 may contribute to calcium regulation in photoreceptors and bipolar cells.

show abstract

Section: Light Microscopic Localization Of Sercas In the Mammalian Rementioning

confidence: 56%

Section: Serca2 Is Prominently Expressed In the Mammalian Retinamentioning

confidence: 62%

See 1 more Smart Citation

Serca isoform expression in the mammalian retina

Križaj¹

2005

Experimental Eye Research

View full text Add to dashboard Cite

show abstract

“…Resequestration of calcium into the SER in PN dendrites is mediated by the sarco/endoplasmic reticulum Ca 2+ ATPase pump (SERCA), the 2b isoform being predominant in PN [49]. Dilation of the SER in PN dendrites [50][51][52] may be due to overloading the SER with calcium [53] or depletion of calcium from the SER [54].…”

Section: The Ser In Pnmentioning

confidence: 99%

Ethanol-Induced Alterations in Purkinje Neuron Dendrites in Adult and Aging Rats: a Review

Dlugos

2015

Cerebellum

View full text Add to dashboard Cite

Uncomplicated alcoholics suffer from discrete motor dysfunctions that become more pronounced with age. These deficits involve the structure and function of Purkinje neurons (PN), the sole output neurons from the cerebellar cortex. This review focuses on alterations to the PN dendritic arbor in the adult and aging Fischer 344 rat following lengthy alcohol consumption. It describes seminal studies using the Golgi-Cox method which proposed a model for ethanol-induced dendritic regression. Subsequent ultrastructural studies of PN dendrites showed dilation of the extensive smooth endoplasmic reticulum (SER) which preceded and accompanied dendritic regression. The component of the SER that was most affected by ethanol was the sarco/endoplasmic reticulum Ca(2+) ATPase pump (SERCA) responsible for resequestration of calcium into the SER. Ethanol-induced decreases in SERCA pump levels, similar to the finding of SER dilation, preceded and occurred concomitantly with dendritic regression. Discrete ethanol-induced deficits in balance also accompanied these decreases. Ethanol-induced ER stress within the SER of PN dendrites was proposed as an underlying cause of dendritic regression. It was recently shown that increased activation of caspase 12, inherent to the ER, occurred in PN of acute slices in ethanol-fed rats and was most pronounced following 40 weeks of ethanol treatment. These findings shed new light into alcohol-induced disruption in PN dendrites providing a new model for the discrete but critical changes in motor function in aging, adult alcoholics.

show abstract

“…In the molecular pathology of these two diseases, the epidermal Ca 2+ gradient is often confused regarding the similar distribution and role of SERCA and SPCA [13]. However, because SERCA2b is expressed at relatively high levels in neurons in the brain [92], the incidence of neuropsychiatric disorders is increased in some Darier's patients [93]. …”

Section: Diseases Related To Ga Ca2+ Regulationmentioning

confidence: 99%

The Golgi Apparatus: Panel Point of Cytosolic Ca²⁺Regulation

Tian

Jiang

et al. 2013

Neurosignals

View full text Add to dashboard Cite

TheGolgi apparatus (GA), an intermediate organelle of the cell inner membrane system, plays a key role in protein glycosylation and secretion. In recent years, this organelle has been found to act as a vital intracellular Ca²⁺ store because different Ca²⁺ regulators, such as the inositol-1,4,5-triphosphate receptor, sarco/endoplasmic reticulum Ca²⁺-ATPase and secretory pathway Ca²⁺-ATPase, were demonstrated to localize on their membrane. The mechanisms involved in Ca²⁺ release and uptake in the GA have now been established. Here, based on careful backward looking on compartments and patterns in GA Ca²⁺ regulation, we review neurological diseases related to GA calcium remodeling and propose a modified cytosolic Ca²⁺ adjustment model, in which GA acts as part of the panel point.

show abstract

Distribution of the organellar Ca2+ transport ATPase SERCA2 isoforms in the cat brain

Cited by 42 publications

References 18 publications

Serca isoform expression in the mammalian retina

Serca isoform expression in the mammalian retina

Ethanol-Induced Alterations in Purkinje Neuron Dendrites in Adult and Aging Rats: a Review

The Golgi Apparatus: Panel Point of Cytosolic Ca²⁺Regulation

Contact Info

Product

Resources

About

Distribution of the organellar Ca2+ transport ATPase SERCA2 isoforms in the cat brain

Cited by 42 publications

References 18 publications

Serca isoform expression in the mammalian retina

Serca isoform expression in the mammalian retina

Ethanol-Induced Alterations in Purkinje Neuron Dendrites in Adult and Aging Rats: a Review

The Golgi Apparatus: Panel Point of Cytosolic Ca2+Regulation

Contact Info

Product

Resources

About

The Golgi Apparatus: Panel Point of Cytosolic Ca²⁺Regulation