Distribution of the Pathogenicity Islands Markers (PAIs) in Uropathogenic E.coli Isolated from Children in Mofid Children Hospital

Abstract

Background: Uropathogenic Escherichia coli (UPEC) is a causative agent of most of urinary tract infections (UTIs), which expresses a multitude of virulence factors. Objectives: The aim of this study was to investigate virulence associated characteristics characteristics in UPEC isolates derived from urine specimens, and to investigate the distribution of the pathogenicity islands virulence markers (PAIs) among the isolates in relation to their antibiotic susceptibility pattern. Patients and Methods: A total of 50 E.coli isolates were collected from patients with UTI during September 2009 to August 2010. Biochemical and standard microbiological techniques were used to identify E.coli followed by screening for islands by polymerase chain reaction (PCR). Results: We found a high number of PAI markers such as PAI ICFT073, PAI IICFT073, PAI I536, PAI IV536, PAI II J96, and PAI II536 significantly associated with UPEC. High level of resistance to Aztreonam, Co-trimoxazole, Cefpodoxime, and Cefotaxime was found among the UPEC isolates. Conclusions: This observation is of special value considering that the UPEC pathotype constitutes an emerging group of enteropathogens, particularly, in our county. Knowledge of the molecular details of Uropathogenic E.coli is useful to develop successful strategies for the treatment of urinary tract infection and complications associated with UTIs in human.

Virulence Genes and Antimicrobial Resistance Pattern in Uropathogenic Escherichia coli Isolated From Hospitalized Patients in Kashan, Iran

“…The present study showed high resistance to aztreonam, which was in accordance with the findings of Navidinia et al who demonstrated that 78% of UPEC strains were resistant to aztreonam ( 23 ). High resistance to aztreonam was reported by Pobiega et al among the extended-spectrum β-lactamases (ESBL)-producing E. coli strains ( 33 ).…”

“…Johnson et al ( 10 ), reported a frequency of 71% for PAI - markers among UPEC isolated from the patients with urosepsis in the USA. In a survey conducted by Navidinia et al ( 23 ), a prevalence of 89% was documented for these markers in UPEC strains. It is documented that the pathogenicity islands (PAIs) capable of horizontal virulence genes transfer between species; therefore, a frequency of 61.3% for PAI markers in UPEC strains isolated from hospitalized patients is notable.…”

<p>Virulence and Resistance Determinants of Uropathogenic <em>Escherichia coli</em> Strains Isolated from Pregnant and Non-Pregnant Women from Two States in Mexico</p>

“…This result coincides with the reported by other authors in Spain, Iran and Iraq. [58][59][60] However, in our work, in addition to high percentage of CFT073 islands, we found, a higher percentage of PAIs (I and II) from E. coli J96 in both states, than the authors above mentioned. These results suggest that some E. coli strains isolated from Mexican patients with UTIs have PAIs more associated to E. coli J96 than the strains isolated in the mentioned countries, although more studies need to be done in Mexico to reinforce our hypothesis.…”

Evaluation of clonal relatedness among different sources of E. coli isolates in Iranian children with urinary tract infection (UTI) and age-matched healthy people.

“…The mechanisms underlying the evolution and emergence of such new bacterial pathogens are not well understood, and these atypical strains now are attracting more attention. The variety of virulence determinants due to horizontal gene transfers of mobile elements, such as pathogenicity islands (PAIs), phages and plasmids contributing to a strain's pathogenic potential [6][7][8][9][10].…”