Distribution of the verrucomicrobial clade <scp><i>S</i></scp><i>partobacteria</i> along a salinity gradient in the <scp>B</scp>altic Sea

Bergen, Benjamin; Herlemann, Daniel P. R.; Labrenz, Matthias; Jürgens, Klaus

doi:10.1111/1758-2229.12178

Cited by 24 publications

(19 citation statements)

References 24 publications

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“…However, the relative read abundances detected by amplicon sequencing were on average about one-third lower than the relative cell abundances determined by CARD-FISH. This was in contrast to the results in a study of Spartobacteria within the salinity gradient of the Baltic Sea, in which the relative number of amplicon sequencing reads was four times higher than the CARD-FISH counts [4]. Since representative genomes from Spartobacteria and "Pelagibacterales" contain only one 16S rRNA gene [13,23], factors other than the 16S rRNA copy number must be responsible for the differences between the amplicon sequencing results and those of CARD-FISH.…”

Section: Resultscontrasting

confidence: 87%

Diversity and abundance of “Pelagibacterales” (SAR11) in the Baltic Sea salinity gradient

Herlemann

Woelk

Labrenz

et al. 2014

Systematic and Applied Microbiology

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The candidate order "Pelagibacterales" (SAR11) is one of the most abundant bacterial orders in ocean surface waters and, periodically, in freshwater lakes. The presence of several stable phylogenetic lineages comprising "Pelagibacterales" correlates with the physico-chemical parameters in aquatic environments. A previous amplicon sequencing study covering the bacterial community in the salinity gradient of the Baltic Sea suggested that pelagibacteral subclade SAR11-I was replaced by SAR11-IIIa in the mesohaline region of the Baltic Sea. In this current study, we investigated the cellular abundances of "Pelagibacterales" subclades along the Baltic Sea salinity gradient using catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). The results obtained with a newly designed probe, which exclusively detected SAR11-IIIa, were compared to CARD-FISH abundances of the marine SAR11-I/II subclade and the freshwater lineage SAR11-IIIb (LD12). The results showed that SAR11-IIIa was abundant in oligohaline-mesohaline conditions (salinities 2.7-13.3), with maximal abundances at a salinity of 7 (up to 35% of total Bacteria, quantified with a universal bacterial probe EUB). As expected, SAR11-I/II was abundant (27% of EUB) in the marine parts of the Baltic Sea, whereas counts of the freshwater lineage SAR11-IIIb were below the detection limit at all stations. The shift from SAR11-IIIa to SAR11-I/II was confirmed in the vertical salinity gradient in the deeper basins of the Baltic Sea. These findings were consistent with an overlapping but defined distribution of SAR11-I/II and SAR11-IIIa in the salinity gradient of the Baltic Sea and suggested the adaptation of SAR11-IIIa for growth and survival in mesohaline conditions.

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Section: Resultscontrasting

confidence: 87%

Diversity and abundance of “Pelagibacterales” (SAR11) in the Baltic Sea salinity gradient

Herlemann

Woelk

Labrenz

et al. 2014

Systematic and Applied Microbiology

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“…Similar trends have also been documented in the Chesapeake Bay (Bouvier and del Giorgio, 2002) and the Pearl River Estuary (Zhang et al, 2006). Verrucomicrobia, however, have previously been shown to peak in brackish conditions in the Baltic Sea in several separate studies (Herlemann et al, 2011;Bergen et al, 2014), though different lineages of the Verrucomicrobia have been detected in diverse environments (Freitas et al, 2012). With the exception of the Verrucomicrobia, our data is in agreement with previous studies showing that salinity is a primary stressor for many phyla of particle-associated bacteria.…”

Section: Bacterial Community Compositionsupporting

confidence: 92%

Impacts of Salinity and Oxygen on Particle-Associated Microbial Communities in the Broadkill River, Lewes DE

2018

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Particulate matter in estuarine systems hosts microbial communities that can impact biogeochemical cycles. While the bacterial community composition on suspended particles has been previously investigated, especially with regards to how salinity may structure these communities, the archaeal fraction of the microbial community has not received the same attention. Here we investigate both the bacterial and archaeal community composition on two sizes of particles along a riverine discharge gradient in the Broadkill River, DE, USA, to determine whether the archaeal community is selected by similar environmental stressors as the bacteria. We measured salinity, nutrients, and diatom abundances, and use particle size as a proxy for oxygen concentrations. We show that salinity is a strong environmental factor that controls both bacterial and archaeal community composition and oxygen is an additional factor, impacting archaea more than bacteria.

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“…In our analysis, Verrucomicrobia , and specifically those OTUs assigned to Spartobacteria , were particularly abundant in the brackish zone in July and February ( Figure 5 ). Spartobacterial OTUs are known to co-occur with phytoplankton blooms (Herlemann et al, 2013; Lindh et al, 2013; Bergen et al, 2014) which are highly abundant in the brackish part of the Baltic Sea (Wasmund et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

“…We also identified typical mesohaline bacterial members in the central Baltic Sea, including the verrucomicrobial taxon “ Candidatus Spartobacterium balticum” (Herlemann et al, 2013; Bergen et al, 2014) and the “SAR11-IIIa” clade (Herlemann et al, 2014). Here, we extend these earlier analyses by analyzing a transect dataset sampled in winter and comparing bacterial community composition in winter and summer along the salinity gradient of the Baltic Sea.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Signals of Salinity and Season in Bacterial Community Composition Across the Salinity Gradient of the Baltic Sea

et al. 2016

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Understanding the key processes that control bacterial community composition has enabled predictions of bacterial distribution and function within ecosystems. In this study, we used the Baltic Sea as a model system to quantify the phylogenetic signal of salinity and season with respect to bacterioplankton community composition. The abundances of 16S rRNA gene amplicon sequencing reads were analyzed from samples obtained from similar geographic locations in July and February along a brackish to marine salinity gradient in the Baltic Sea. While there was no distinct pattern of bacterial richness at different salinities, the number of bacterial phylotypes in winter was significantly higher than in summer. Bacterial community composition in brackish vs. marine conditions, and in July vs. February was significantly different. Non-metric multidimensional scaling showed that bacterial community composition was primarily separated according to salinity and secondly according to seasonal differences at all taxonomic ranks tested. Similarly, quantitative phylogenetic clustering implicated a phylogenetic signal for both salinity and seasonality. Our results suggest that global patterns of bacterial community composition with respect to salinity and season are the result of phylogenetically clustered ecological preferences with stronger imprints from salinity.

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Distribution of the verrucomicrobial clade Spartobacteria along a salinity gradient in the Baltic Sea

Cited by 24 publications

References 24 publications

Diversity and abundance of “Pelagibacterales” (SAR11) in the Baltic Sea salinity gradient

Diversity and abundance of “Pelagibacterales” (SAR11) in the Baltic Sea salinity gradient

Impacts of Salinity and Oxygen on Particle-Associated Microbial Communities in the Broadkill River, Lewes DE

Phylogenetic Signals of Salinity and Season in Bacterial Community Composition Across the Salinity Gradient of the Baltic Sea

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