Distribution of Theta Antigens in Ovum Fusion Derived AKR → CBA/H‐T6T6 Tetraparental Mouse Chimaeras

Abstract

The distribution of antigens has been studied on blood lymphocytes of eight AKR++ CBA/ H-ToTa chimaeras using immunoautoradiography and of male hybrids born from the mixed germ line AKR++CBAiH-ToTfi chimaera X Balbic involving the cytotoxicity method. Analysis of 0 positive cells in the peripheral blood of the tetraparental chimaeras showed that the sum of both 0 positive cell populations varied between 49 and 81 % of the total blood lymphocytes. In spite of this, there was a marked variation in the proportion of the two cell populations ~ in some chimaeras ARK 8 positive cells predominated, in others the reverse was true. I n hybrids it is apparent that both antigens were detected, and furthermore, the total percentage exceeded 100 %I. The quantitative distribution of both 0 antigens is held to reflect the contribution of each genotype in the general make-up of the total somatic population and it is concluded that the ovum fusion has not resulted in cell hybridization.

Chimeric drift and immunological tolerance in allophenic mice

“…(Munro et al, 1974a). One previous study suggests that there is no fusion of cells in allophenic mice (Bona et al, 1974). However, it is clear from these data that the vast majority of cells in these chimeric animals have one or the other parental type of cell surface antigens.…”

Chimeric drift and immunological tolerance in allophenic mice

“…ber of parental cells in allophenic mice has never been vigorously excluded (Munro et al, 1974a). One previous study suggests that there is no fusion of cells in allophenic mice (Bona et al, 1974). However, it is clear from these data that the vast majority of cells in these chimeric animals have one or the other parental type of cell surface antigens.…”

“…in the scientific fields of experimental embryology, genetics, and immun ology. Some of the markers used to identify the different cell popula tions in an allophenic mouse include cell surface antigens (Mintz and Palm, 1969;Mintz and Silvers, 1970;Bona et al, 1974;Festenstein et al, 1975;Warner et al, 1976Warner et al, , 1977aand Stephens et al, 1977), hemoglobin 3-chain differences (Wegmann and Gilraan, 1970;Stephens et al, 1977;and Warner et al, 1977d), biochemical variations of the enzymes glucose phosphate isomerase (GPI) and isocitrate dehydrogenase (IDH) (Gearhart and Mintz, 1972;Chapman et al, 1972;Mintz and Baker, 1967;and Ansell et al, 1974), and chromosomal markers such as the T6 chromo some translocation (Mystkowska and Tarkowski, 1968;Ford et al, 1975). Tarkowski, 1970;Milet et al, 1972;McLaren, 1975;and Ford et al, 1975).…”