Distribution of TRPV1 and TRPV2 in the human stellate ganglion and spinal cord

Kokubun, Souichi; Sato, Tadasu; Ogawa, Chikara; Kudo, Kai; Goto, Koju; Fujii, Yuki; Shimizu, Yoshinaka; Ichikawa, Hiroyuki

doi:10.1016/j.neulet.2015.01.074

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…Other limitations in this study were that (i) the majority of FD patients presenting with PDS, therefore we may not be able to elucidate the full picture of all FD subtypes. (ii) We could only obtain the projected expression of TRPV1 and TRPV2 from the gastric mucosa during endoscopy where TRPV originates from extrinsic sensory neurons (eg, dorsal root ganglion) . (iii) We were unable to perform a complete profile of RT‐PCR and protein assay for all biomarkers (eg, gastric MCP‐1) due to limited sample collections.…”

Section: Discussionmentioning

confidence: 99%

Up‐regulation of transient receptor potential vanilloid (TRPV) and down‐regulation of brain‐derived neurotrophic factor (BDNF) expression in patients with functional dyspepsia (FD)

Cheung

Lin

Kyaw

et al. 2017

Neurogastroenterology Motil

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Section: Discussionmentioning

confidence: 99%

Up‐regulation of transient receptor potential vanilloid (TRPV) and down‐regulation of brain‐derived neurotrophic factor (BDNF) expression in patients with functional dyspepsia (FD)

Cheung

Lin

Kyaw

et al. 2017

Neurogastroenterology Motil

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“…Nonetheless, electrophysiological properties of these receptors differed from those of TRPV1 present in DRG neurons, and only receptors located in DRG responded to capsaicin. Moreover, Kokubun et al (2015) using immunohistochemistry have found that TRPV1 are present in 25.3 % of stellate ganglion sympathetic neurons in human cadavers. These data (unfortunately fragmentary) may suggest another possible mechanism which should be taken into account while discussing the present results-a direct one involving TRPV1 receptors that potentially may be located on SChG-UBPN in the pig.…”

Section: Discussionmentioning

confidence: 99%

The influence of intravesical administration of resiniferatoxin (RTX) on the chemical coding of sympathetic chain ganglia (SChG) neurons supplying the porcine urinary bladder

Lepiarczyk

Majewski

Bossowska

2015

Histochem Cell Biol

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Resiniferatoxin (RTX) is used as an experimental drug in therapy of neurogenic urinary bladder disorders. The present study investigated the chemical coding of sympathetic chain ganglia (SChG) neurons supplying porcine urinary bladder after intravesical RTX instillation. The SChG neurons were visualized with retrograde tracing method and their chemical profile was disclosed with double-labeling immunohistochemistry using antibodies against dopamine β-hydroxylase (DβH; marker of noradrenergic neurons), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin, Leu(5)-enkephalin and neuronal nitric oxide synthase (nNOS). It was found that in both the control (n = 5) and RTX-treated pigs (n = 5), the vast majority (90.4 ± 2.8 and 89.7 ± 2.3%, respectively) of FB-positive (FB+) nerve cells were DβH+. RTX instillation caused a decrease in the number of FB+/DβH+ neurons immunopositive to NPY (71.1 ± 12.1 vs 43.2 ± 6.7%), VIP (21.3 ± 10.7 vs 5.3 ± 4.3%) or SOM (16.5 ± 4.6 vs 2.3 ± 2.6%) and a distinct increase in the number of FB+/DβH+ neurons immunoreactive to nNOS (0.8 ± 1 vs 5.3 ± 1.9 %). The present study for the first time has provided some information that therapeutic effects of RTX on the mammalian urinary bladder can be partly mediated by SChG neurons.

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“…We generated a family of amplicon plasmids (the pA5 family) that express firefly luciferase (FLuc) under the control of rTRPV-1, rCGRP, and rASIC3 sensory neuron-specific promoters (rat promoters). The selection of these promoters was based on the results of a non-systematic literature review [ 7 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ] that we conducted to identify proteins expressed in sensory neurons that innervate the bladder, but not (or weakly) expressed in parasympathetic or sympathetic neurons. The relative expression of these proteins should increase in sensory neurons after SCI.…”

Section: Resultsmentioning

confidence: 99%

Development and Assessment of Herpes Simplex Virus Type 1 (HSV-1) Amplicon Vectors with Sensory Neuron-Selective Promoters

Joussain

Coz

Pichugin

et al. 2022

IJMS

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Background: Neurogenic detrusor overactivity (NDO) is a severe pathological condition characterized by involuntary detrusor contractions leading to urine leakage. This condition is frequent after spinal cord injury (SCI). Gene therapy for NDO requires the development of vectors that express therapeutic transgenes driven by sensory neuron-specific promoters. The aim of this study was to develop and assess tools for the characterization of sensory neuron-specific promoters in dorsal root ganglia (DRG) neurons after transduction with herpes simplex virus type 1 (HSV-1)-based amplicon defective vectors. Methods: The HSV-1 vector genome encoded two independent transcription cassettes: one expressed firefly luciferase (FLuc) driven by different promoters’ candidates (rTRPV1, rASIC3, rCGRP, or hCGRP), and the other expressed a reporter gene driven by an invariable promoter. The strength and selectivity of promoters was assessed in organotypic cultures of explanted adult DRG, or sympathetic and parasympathetic ganglia from control and SCI rats. Results: The rCGRP promoter induced selective expression in the DRG of normal rats. The rTRPV-1 promoter, which did not display selective activity in control rats, induced selective expression in DRG explanted from SCI rats. Conclusions: This study provides a methodology to assess sensory neuron-specific promoters, opening new perspectives for future gene therapy for NDO.

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Distribution of TRPV1 and TRPV2 in the human stellate ganglion and spinal cord

Cited by 10 publications

References 26 publications

Up‐regulation of transient receptor potential vanilloid (TRPV) and down‐regulation of brain‐derived neurotrophic factor (BDNF) expression in patients with functional dyspepsia (FD)

Up‐regulation of transient receptor potential vanilloid (TRPV) and down‐regulation of brain‐derived neurotrophic factor (BDNF) expression in patients with functional dyspepsia (FD)

The influence of intravesical administration of resiniferatoxin (RTX) on the chemical coding of sympathetic chain ganglia (SChG) neurons supplying the porcine urinary bladder

Development and Assessment of Herpes Simplex Virus Type 1 (HSV-1) Amplicon Vectors with Sensory Neuron-Selective Promoters

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