Distribution of tyrosine hydroxylase(TH)-like, neuropeptide Y(NPY)-like immunoreactive and acetylcholinesterase(AChE)-positive nerve fibers in the prostate gland of the monkey (macacus fuscatus).

Yokoyama, Ryuji; Inokuchi, Tetsuo; Satoh, Hiromi; Kusaba, Tooru; Yamamoto, Kazumi; Andō, Kōichi

doi:10.2739/kurumemedj.37.1

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Published data on the autonomic nerves in human prostate indicate that adrenergic nerves are found in stroma, whereas parasympathetic acetylcholine nerves are projecting to secretory acini. 32 , 33 Similar conclusions were made from the analysis of prostates of Japanese monkeys 34 and in rats. 1 , 35 These neuroanatomic studies suggest that there are no direct contacts between sympathetic adrenergic nerves and prostate epithelium.…”

Section: Discussionsupporting

confidence: 57%

Inhibition of signaling downstream of beta‐2 adrenoceptor by propranolol in prostate cancer cells

et al. 2022

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Background: There is accumulating evidence that propranolol, an antagonist of beta-1 and beta-2 adrenoreceptors, extends survival of patients with prostate cancer; yet it is not known whether propranolol inhibits beta-adrenergic signaling in prostate cancer cells, or systemic effects of propranolol play the leading role in slowing down cancer progression. Recently initiated clinical studies offer a possibility to test whether administration of propranolol inhibits signaling pathways in prostate tumors, however, there is limited information on the dynamics of signaling pathways activated downstream of beta-2 adrenoreceptors in prostate cancer cells and on the inactivation of these pathways upon propranolol administration.Methods: Western blot analysis was used to test the effects of epinephrine and propranolol on activation of protein kinase (PKA) signaling in mouse prostates and PKA, extracellular signal-regulated kinase (ERK) , and protein kinase B/AKT (AKT) signaling in prostate cancer cell lines.Results: In prostate cancer cell lines epinephrine induced robust phosphorylation of PKA substrates pS133CREB and pS157VASP that was evident 2 min after treatments and lasted for 3−6 h. Epinephrine induced phosphorylation of AKT in PTEN-positive 22Rv1 cells, whereas changes of constitutive AKT phosphorylation were minimal in PTEN-negative PC3, C42, and LNCaP cells. A modest short-term increase of pERK in response to epinephrine was observed in all tested cell lines. Incubation of prostate cancer cells with 10-fold molar excess of propranolol for 30 min inhibited all downstream pathways activated by epinephrine. Subjecting mice to immobilization stress induced phosphorylation of S133CREB, whereas injection of propranolol at 1.5 mg/kg prevented the stress-induced phosphorylation.Conclusions: The analysis of pS133CREB and pS157VASP allows measuring activation of PKA signaling downstream of beta-2 adrenoreceptors. Presented results on the ratio of propranolol/epinephrine and the time needed to inhibit

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Section: Discussionsupporting

confidence: 57%

Inhibition of signaling downstream of beta‐2 adrenoceptor by propranolol in prostate cancer cells

et al. 2022

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“…Muscarinic receptors (m3 subtype) have been localized both in the stroma and epithelia of the prostate gland [35] and are also expressed in rat prostate cancer cell lines [62]. Sympathetic cholinergic nerves exhibit an intimate spatial association with the glandular epithelium of the prostate [63] and muscarinic agonists directly stimulate cellular secretion in the gland [15]. Proliferation of both primary cultures and cell lines of human prostate cancer cells is stimulated by muscarinic receptor activation [64].…”

Section: Acetylcholinementioning

confidence: 99%

Ionic and pharmacologic characteristics of epithelial cells in a semi‐intact preparation of the rat ventral prostate gland

Mycielska¹,

Szatkowski²,

Djamgoz³

2002

The Prostate

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“…Besides noradrenaline Immunohistochemical properties of nerve fibres supplying accessory male genital glands in the pig. A colocalisation study (and presumably acetylcholine), a variety of other substances have been identified in these nerves, such as neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP), enkephalins (ENK), somatostatin (SOM), serotonin, peptide histidine isoleucine, gastrin-releasing peptide, bombesin, pituitary adenylate cyclase-activating peptide, helospectin and nitric oxide synthase (Alm et al 1978(Alm et al , 1980(Alm et al , 1981Vaalasti et al 1980Vaalasti et al , 1986Gu et al 1983;Stjernquist et al 1983;Adrian et al 1984;Lamano Carvalho et al 1986;Higgins and Gosling 1989;Lange and Unger 1990;Yokoyama et al 1990;Yuri 1990;Crowe et al 1991;Properzi et al 1992;Iravani and Zar 1994;Jen et al 1995Jen et al , 1996Tainio 1995;Afonso et al 1996;Hedlund et al 1996;Chow et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical properties of nerve fibres supplying accessory male genital glands in the pig. A colocalisation study

Kaleczyc¹,

Timmermans

Majewski

et al. 1999

Histochemistry and Cell Biology

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Immunohistochemical studies have been performed to investigate the occurrence and coexistence of two catecholamine-synthesising enzymes, tyrosine hydroxylase and dopamine-beta-hydroxylase, and several neuropeptides, including neuropeptide Y, vasoactive intestinal polypeptide, Leu5-enkephalin, somatostatin, calcitonin gene-related peptide and substance P, in nerve fibres supplying porcine accessory genital glands, the seminal vesicles, prostate (body and the disseminated part) and bulbourethral glands. Three major populations of nerve fibres supplying non-vascular elements of the glands have been distinguished (from the largest to the smallest one): (1) noradrenergic fibres, the majority of which contain Leu5-enkephalin, neuropeptide Y or, to a lesser extent, somatostatin, (2) non-noradrenergic, putative cholinergic fibres containing vasoactive intestinal polypeptide, neuropeptide Y and/or somatostatin and, (3) nonnoradrenergic, presumably sensory fibres, containing calcitonin gene-related peptide and substance P. Whilst the coexistence patterns within nerves supplying particular glands are similar, the density of innervation varies between the organs. The innervation of the seminal vesicles and prostatic body is more developed than that of the disseminated part of the prostate and bulbourethral glands. The majority of noradrenergic fibres related to blood vessels contain neuropeptide Y only, while the non-noradrenergic nerves contain mainly vasoactive intestinal polypeptide. The possible function and origin of particular nerve fibre populations are discussed.

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Distribution of tyrosine hydroxylase(TH)-like, neuropeptide Y(NPY)-like immunoreactive and acetylcholinesterase(AChE)-positive nerve fibers in the prostate gland of the monkey (macacus fuscatus).

Cited by 7 publications

References 25 publications

Inhibition of signaling downstream of beta‐2 adrenoceptor by propranolol in prostate cancer cells

Inhibition of signaling downstream of beta‐2 adrenoceptor by propranolol in prostate cancer cells

Ionic and pharmacologic characteristics of epithelial cells in a semi‐intact preparation of the rat ventral prostate gland

Immunohistochemical properties of nerve fibres supplying accessory male genital glands in the pig. A colocalisation study

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