Distribution of Vibrio Parahaemolyticus in Plankton and Fish in the Open Sea

Abstract

In the previous oceanographic survey conducted by HORIE et al .8) it was revealed that Biotype 1 organisms of Vibrio parahaemolyticus distribute in coastal sea area. It was also noted that Biotype 1 organisms can be more frequently isolated from plankton or marine mud than from sea water sample. The present study was undertaken to investigate the occurrence of Biotype 1 or 2 organ isms in the plankton or fish inhabiting in the pelagic ocean. The survey was carried out aboard the Umitaka-maru, a research vessel of Tokyo University of Fisheries in the sea off Southern Kanto and Tokai districts late in July 1963. The location of sampling stations is shown in Fig. 1. Plankton samples were obtained at all the 13 stations , whereas young fish were caught only at 3 stations. At the station 9, particularly , several samples of sizable fish and squid could be caught using flyingfish drift net , and the gastric contents of these organ isms were tested. A selective liquid medium, 3% NaCl containing arabinose ethyl violet broth (AE medium) , as shown in Table 1, was used. In order to get higher rate of detection of Biotype 1 organ isms, ME medium, 3% NaCl containing mannitol ethyl violet broth was employed , and the said medium of pH 7.8 was used in parallel with that of pH 9 .0. No bacteria belonging to Biotype 1 could be detected from the samples collected i n the pelagic ocean, whereas Biotype 2 were found to be widely distributed in the plankton samples. Bi otype 3 bacteria was not isolated from the plankton , but detected in the samples of young fish and in the gastric contents of fish and squid. Some strains isolated from the flying fish could be classified as Biotype 2 on account of the salt resistance , positive Voges-Proskauer reaction and sucrose fermentation, however , they were differentiated from the typical organ isms of Biotype 2 by other biological features.

The distribution ofVibrio parahaemolyticusin British coastal waters: report of a collaborative study 1975–6

“…During (Cann, 1976 (Hori et al, 1964;Hechelman, Asakawa & Leistner, 1969, Varga & Hirthe, 1975 which suggest that it is largely confined to coastal areas. Although Golten & Scheffers (1975) failed to isolate V. parahaemolyticus from samples of water from the Dutch coast during mid-summer, Kampelmacher, van Noorle Jansen, Mossel & Groen (1972) isolated the organism from larger volumes of sediment-rich estuarine vTater early in the year.…”

Microbiological quality of retail fresh fish fillets in The Netherlands

“…Vibrio parahaemolyticus was initially (1977)(1978) searched for in 10 g of fish fillet by enrichment in 5% NaCl-Meat Broth for 18-24 h at 37°C (Kampelmacher et al, 1970(Kampelmacher et al, , 1972. After this technique had been found insufficiently productive (van den Broek et al, 1979a) 110 samples were resuscitated in Tryptone Soya Broth (Oxoid CM 129) with 2% NaC1 for 2 h at room temperature and then cultured overnight in Horie's Arabinose Ethylviolet Broth (Horie et al, 1964) at 37°C. Subcultures were in all instances made on TCBS-Agar (Oxoid CM 333) incubated overnight at 37°C (Kampelmacher et al, 1970(Kampelmacher et al, , 1972.…”

Occurrence of Vibrio parahaemolyticus in Dutch mussels

“…As a comparison, the enrichment media were also inoculated directly. Besides the salt meat broth used in survey 1, the arabinose-ethyl violet broth of Horie et al (11) was also used. This was because Beuchat (5, 6) found the latter broth most suitable for detecting uninjured, heat-stressed, chilled, and frozen V. parahaemolyticus.…”