Distribution of virulence genes and biofilm characterization of human isolates of Streptococcus agalactiae: A pilot study

Balasubramanian, N; Pounpandi, P.; Varatharaju, G.; Shanmugaiah, V.; Balakrishnan, K.; Thirunarayan, MA.

doi:10.1016/j.colsurfb.2023.113151

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…For the SAT assay, primers and probes were designed to be speci c for the mRNA of the GBS cfb gene. The cfb gene of GBS has been used in the detection of GBS in multiple studies, and it has been shown to be highly conserved (13).…”

Section: Primers and Probesmentioning

confidence: 99%

To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays

Lu,

Chen,

Wang

et al. 2024

Preprint

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Background To evaluate the performance of simultaneous amplification and testing (SAT) assay for the detection of group B Streptococcus (GBS) in maternal vaginal and perianal swabs compared with real-time polymerase chain reaction (RT‒PCR). Methods We obtained vaginal and perianal swabs from 1474 pregnant women at the Obstetrics and Gynecology Hospital of Fudan University (Shanghai, China) between April 2023 and June 2023. Vaginal and perianal swabs were collected at 35–37 weeks of gestation. Swabs were tested for GBS simultaneously by using the SAT assay and RT‒PCR, and a comparative analysis (kappa coefficient) was performed. Furthermore, we conducted additional droplet digital PCR (ddPCR) tests to confirm the results when there were controversial results between SAT and RT‒PCR. In addition, we compared the limit of detection, technical specificity, repeatability and reproducibility of SAT-GBS with those of routine RT‒PCR assays. Results In our study, the rate of clinical GBS colonization according to the SAT assay was 11.5% (169/1471). The SAT assay showed a sensitivity of 91.8%, a specificity of 99.9%, a diagnostic accuracy of 98.9%, a positive predictive value (PPV) of 99.4% and a negative predictive value (NPV) of 98.8%. The kappa value between RT‒PCR and SAT was 0.917. Conclusions This SAT assay for the detection of group B Streptococcus is not only easy to perform but can also detect GBS sensitively and specifically and may be used in the regular molecular diagnosis of GBS in cases of newborn sepsis and meningitis.

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Section: Primers and Probesmentioning

confidence: 99%

To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays

Lu,

Chen,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…This infection can cause symptoms such as vaginal inflammation ( Tano et al, 2021 ), abnormal vaginal discharge ( Dilrukshi et al, 2021 ), and itching ( Tano et al, 2021 ). GBS is also one of the common pathogens that cause urinary tract infections in non-pregnant women ( Balasubramanian et al, 2023 ). Urinary tract infections can cause symptoms such as frequent urination, urgency, and pain during urination.…”

Section: Gbs Related Clinical Diseases In Obstetrics and Gynecologymentioning

confidence: 99%

Current research update on group B streptococcal infection related to obstetrics and gynecology

Liu,

2024

Front. Pharmacol.

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Group B streptococcal (GBS) is a Gram-positive bacterium that is commonly found in the gastrointestinal tract and urogenital tract. GBS infestation during pregnancy is a significant contributor to maternal and neonatal morbidity and mortality globally. This article aims to discuss the infectious diseases caused by GBS in the field of obstetrics and gynecology, as well as the challenges associated with the detection, treatment, and prevention of GBS.

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To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays

Lu,

Chen,

Wang

et al. 2024

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

Background To evaluate the performance of simultaneous amplification and testing (SAT) assay for the detection of group B Streptococcus (GBS) in maternal vaginal and perianal swabs compared with real-time polymerase chain reaction (RT-PCR). Methods We obtained vaginal and perianal swabs from 1474 pregnant women at the Obstetrics and Gynecology Hospital of Fudan University (Shanghai, China) between April 2023 and June 2023. Vaginal and perianal swabs were collected at 35–37 weeks of gestation. Swabs were tested for GBS simultaneously by using the SAT assay and RT-PCR, and a comparative analysis (kappa coefficient) was performed. Furthermore, we conducted additional droplet digital PCR (ddPCR) tests to confirm the results when there were controversial results between SAT and RT-PCR. In addition, we compared the limit of detection, technical specificity, repeatability and reproducibility of SAT-GBS with those of routine RT-PCR assays. Results In our study, the detection rate of clinical GBS according to the SAT assay was 11.5% (169/1471). The SAT assay showed a sensitivity of 91.8%, a specificity of 99.9%, a diagnostic accuracy of 98.9%, a positive predictive value (PPV) of 99.4% and a negative predictive value (NPV) of 98.8%. The kappa value between RT-PCR and SAT was 0.917. Conclusions This SAT assay for the detection of group B Streptococcus is not only easy to perform but can also detect GBS sensitively and specifically and may be used in the regular molecular diagnosis of GBS infection among pregnancies.

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Distribution of virulence genes and biofilm characterization of human isolates of Streptococcus agalactiae: A pilot study

Cited by 4 publications

References 38 publications

To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays

To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays

Current research update on group B streptococcal infection related to obstetrics and gynecology

To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays

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