Distribution of zinedin in the rat brain

Abstract

Members of the striatin family are scaffolding proteins involved in numerous signaling pathways principally in neurons. Zinedin is the only member of this protein family for which the brain distribution has not been determined so far. Here, we have validated a specific antibody against zinedin and used this tool to study the localization of zinedin at cellular and subcellular levels in the rat brain. Zinedin is primarily expressed in neurons of the hippocampus, cerebral cortex, olfactory bulb and caudate putamen nucleus. Like other members of the striatin family, zinedin displays a polarized distribution in the somato-dendritic compartment of neurons and is enriched in dendritic spines. The rostral expression of zinedin as well as its compartmented distribution in dendritic spines may have important implications not only for zinedin function but also in the physiology of dendritic spines of a particular subset of neurons.

The phocein homologue SmMOB3 is essential for vegetative cell fusion and sexual development in the filamentous ascomycete Sordaria macrospora

“…The mammalian striatin family comprises the proteins striatin, zinedin and SG2NA, which are mainly expressed in neurons of the central nervous system. Within neurons, they display a typical polarized somato-dendritic localization, are absent from axons, and are highly concentrated in dendritic spines (Benoist et al 2008; Castets et al 1996; Gaillard et al 2006; Kachidian et al 1998). …”

Molecular and structural bases for postsynaptic signal processing: interaction between postsynaptic density and postsynaptic membrane rafts

“…32,33 The isolation of PSD-PSR complexes in our study was expected, because PSRs are relatively stably connected to PSDs and may provide attachment sites for PSD cytoskeletons/scaffolds. The close association between PSDs and PSRs is supported by reports showing localization of typical raft proteins, such as caveolin-1, 34 striatin, 35,36 zinedin, 37 paralemmin, 38 and Abbreviations: SDG, sucrose density gradient; ppt, pellet; PSD, postsynaptic density; PSR, postsynaptic membrane raft; SPM, synaptic plasma membrane; DRM, detergentresistant membrane.…”

STRIPAK complexes: Structure, biological function, and involvement in human diseases

“…1) (Castets et al, 2000). In brain, zinedin is expressed most abundantly in the hippocampus (Benoist et al, 2008). Similar to other striatin family members, zinedin shows somato-dendritic localization in neurons with high concentration in dendritic spines and is expressed in a variety of other tissues (Benoist et al, 2008, Castets et al, 2000, Gaillard et al, 2006, Gordon et al, 2011).…”