Distribution of α1‐adrenoceptor subtype mRNAs in human renal cortex

Abstract

Three alpha1-adrenoceptor subtype mRNAs were recognized in human renal cortex and detected particularly in the smooth muscle of the arteries. There was more alpha1a-adrenoceptor subtype in human renal cortex than the other subtypes. It is not known how each subtype operates against adrenergic stimulation; further studies are needed to examine receptor density or receptor function.

Alpha1-Adrenergic Receptor Activation Stimulates Calcium Entry and Proliferation via TRPC6 Channels in Cultured Human Mesangial Cells

“…However, the underlying mechanisms remain poorly defined. The gene expression of all three α 1 -AR subtypes (α 1A , α 1B , and α 1D ) was identified in the kidney cortex [11,12]. Using in situ hybridization, α 1 -AR mRNA staining was detected in glomerular MCs [12].…”

“…The gene expression of all three α 1 -AR subtypes (α 1A , α 1B , and α 1D ) was identified in the kidney cortex [11,12]. Using in situ hybridization, α 1 -AR mRNA staining was detected in glomerular MCs [12]. Prototypically, stimulation of α 1 -AR leads to the activation of G protein-coupled phospholipase Cβ (PLCβ), which catalyzes the formation of inositol 1,4,5-triphosphate (IP 3 ) and diacylglycerol (DAG), thereby causing a release of internal Ca 2+ stores and an accompanying sustained Ca 2+ influx [13,14].…”

Altered Expression Profile of Renalα1D-Adrenergic Receptor in Diabetes and Its Modulation by PPAR Agonists

“…Using in situ hybridization, Kurooka et al identified the gene expression of all three α 1 -AR subtypes in human kidney cortex [ 14 ]. They found that intense α 1 -AR mRNA staining was apparent especially in the smooth muscle of arterial walls, whereas weak staining of each of the α 1 -AR mRNAs was observed in the glomeruli and renal tubules [ 14 ].…”

“…Using in situ hybridization, Kurooka et al identified the gene expression of all three α 1 -AR subtypes in human kidney cortex [ 14 ]. They found that intense α 1 -AR mRNA staining was apparent especially in the smooth muscle of arterial walls, whereas weak staining of each of the α 1 -AR mRNAs was observed in the glomeruli and renal tubules [ 14 ]. More recently, the presence and distribution of subtypes in human renal pelvis and calyces were evaluated, and α 1D -AR was most dense in both followed by α 1A and α 1B [ 8 ].…”

Dopamine D1-like receptors regulate the α1A-adrenergic receptor in human renal proximal tubule cells and D1-like dopamine receptor knockout mice

“…A fourth AR (␣ 1L -AR) represents a functional phenotype of ␣ 1A -ARs (19). Of the three ␣ 1 -ARs, the ␣ 1A -AR is the major receptor subtype that is expressed in renal proximal and distal renal tubules, although to a lesser degree compared with arteries, in the human renal cortex (48). The ␣ 1A -AR is the predominant ␣ 1 -AR subtype that mediates vasoconstrictor responses to exogenous NE in rats (55).…”