Disturbance in Arrangement of Heliozoan Axopodia and Their Degradation with Concanavalin A Treatment&lt;SUB&gt;1&lt;/SUB&gt;

Toyohara, Akira; Shigenaka, Yoshinobu; Utsumi, Kozo

doi:10.1247/csf.2.307

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…This effect was dependent on the colchicine concentration. In a previous study we found that colchicine enhanced membrane fluidity thereby inducing the so-called patching phenomenon or the windmill formation of axopodia in the presence of concanavalin A (20). The fusion reaction in heliozoan cells also is considered to have a close relationship to cell membrane fluidity.…”

Section: Discussionmentioning

confidence: 96%

Studies on the Cell Fusion of Heliozoans. VI. Enhancement of the Fusion Reaction Induced by Cold and Colchicine Treatment

Shigenaka

Toyohara

Suzaki

et al. 1979

Cell Struct. Funct.

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ABSTRACT. Using low temperature and colchicine, we examined how axopodia play a role in the initiation and subsequent process of cell fusion in a large heliozoan Echinosphaerium nucleofilum (strain MA). Although the fusion reaction is naturally temperature-dependent, preliminary treatment with a low temperature (2?) induced the reaction more efficiently than in the control kept at room temperature (2?).During the recovery process from the cold, the reaction was enhanced conspicuously in the presence of colchicine; the fusion indices were 2.05 to 5.68 after a 9 h incubation with 4 to 8 mM colchicine. When treated with 4 to 10 mM colchicine without the preliminary cold treatment, the reaction also was enhanced. This means that the reaction depended upon the colchicine concentration and resulted in partial or complete degradation of axopodia; fusion indices were 1.08 to 3.77 after a 9 h incubation with 4 to 10 mM colchicine. We discuss these results and the electron-microscopical data, in relation to the role of axopodia in the fusion reaction.As indicated by the genus name, the large heliozoan Echinosphaerium is characterized by axopodia radiating from its spherical cell body that contain hundreds of microtubules as cytoskeletal entities. The axopodia have been investigated with respect to their stability and/or lability (1,5,6,8,13), the saltatory movement of intra-axopodial particles such as mitochondria, electron-dense bodies and kinetocysts (2-4, 21), the sliding and rotational locomotion of the organisms (15, 22), and feeding behavior (6). However, it is not yet known how the axopodia play a role in the fusion process in heliozoans.In the present study, therefore, we aimed to clarify the role of axopodia on the fusion process, especially in the very early stage. As tools, anti-mitotic factors such as colchicine and low temperature were used to induce partial or complete degradation of the axopodia and to inhibit their re-extension. We found that the fusion reaction was enhanced by the experimentally-induced axopodial degradation, particularly with the inhibition of axopodial re-extension.

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Section: Discussionmentioning

confidence: 96%

Studies on the Cell Fusion of Heliozoans. VI. Enhancement of the Fusion Reaction Induced by Cold and Colchicine Treatment

Shigenaka

Toyohara

Suzaki

et al. 1979

Cell Struct. Funct.

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“…無殻太陽虫アクチノフィリスのエクストルソームは，針状の仮足である軸足(axopodia)や細胞体の細胞膜直下に存在している 27,52,53) 。アクチノフィリスの餌の捕獲(図２A) は，軸足への餌生物の付着によって開始され，その後，軸足が餌生物とともに収縮し，食胞形成仮足が形成される 53,55) 。この過程で，最大10,000個のエクストルソームが消費されると報告されている 27,53) 。エクストルソームの内部には餌に対する接着物質が存在している 27,55) 23,60,65) 。そこで，アクチノフィリスの Con A 結合性の糖タンパク質を Con A -アフィニティークロマトグラフィーを用いて精製し，その特性と局在性を調べたところ，Con A 結合性の糖タンパク質 gp40がエクストルソームに含まれており，餌生物の認識とそれへの接着およびエンドサイトーシスの誘導に関与する物質であることが示された 56) 。また，アクチノフィリスの餌の捕獲は，Con A によって阻害されることも示された 56)…”

Section: ．アクチノフィリスの捕食機構unclassified

Molecular basis for interactions between protists and other organisms: How protists recognize and capture prey cells

Suzaki

2022

Hikaku seiri seikagaku(Comparative Physiology and Biochemistry)

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“…The degradation process in these labile microtubules has been investigated with emphasis on low temperature (22,24), hydrostatic pressure (23), colchicine (21,24), light metal ions (14,17,18), heavy metal ions (11,12,15), urea (16), various proteins and amino acids (13,25), EDTA (13), inhalational anaesthetics (1), and SH-blocking reagents (19). Moreover, the axonemal microtubules are known to be reversibly reassembled by the cancellation of these various factors.…”

mentioning

confidence: 99%

Microtubules in Protozoan Cells.V. Macrotubule-to-Microtubule Transition during the Recovery Process from Cold and Colchicine Treatment in Heliozoan Cells

Toyohara

Shigenaka

Suzaki

et al. 1980

Cell Struct. Funct.

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Disturbance in Arrangement of Heliozoan Axopodia and Their Degradation with Concanavalin A Treatment<SUB>1</SUB>

Cited by 6 publications

References 29 publications

Studies on the Cell Fusion of Heliozoans. VI. Enhancement of the Fusion Reaction Induced by Cold and Colchicine Treatment

Studies on the Cell Fusion of Heliozoans. VI. Enhancement of the Fusion Reaction Induced by Cold and Colchicine Treatment

Molecular basis for interactions between protists and other organisms: How protists recognize and capture prey cells

Microtubules in Protozoan Cells.V. Macrotubule-to-Microtubule Transition during the Recovery Process from Cold and Colchicine Treatment in Heliozoan Cells

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