Disturbance of chlorophyll biosynthesis at Mg branch affects the chloroplast ROS homeostasis and Ca2+ signaling in Pisum sativum

Luo, Sha; Luo, Tao; Peng, Peng; Li, Yanping; Li, Yong

doi:10.1007/s11240-016-1008-3

Cited by 9 publications

(8 citation statements)

References 34 publications

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“…The total chlorophyll (chlorophyll, a + b) was extracted with 100% acetone, and the concentration was determined spectrophotometrically according to our previous study ( Luo et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

“…It is the only reported subunit modulated by redox regulation via the chloroplast thioredoxin system ( Ikegami et al, 2007 ; Luo et al, 2012 ; Perez-Ruiz et al, 2014 ). In addition, it is also involved in chloroplast reactive oxygen species homeostasis and Ca 2+ signaling in pea ( Luo et al, 2016 ). As a key regulatory point of chlorophyll biosynthesis, the CHLI subunit might be the primary target to engineer at a molecular level.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Mg-Chelatase CHLI Subunit in Pea (Pisum sativum L.)

Wang

Zhu

et al. 2022

Front. Plant Sci.

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As a rate-limiting enzyme for chlorophyll biosynthesis, Mg-chelatase is a promising target for improving photosynthetic efficiency. It consists of CHLH, CHLD, and CHLI subunits. In pea (Pisum sativum L.), two putative CHLI genes (PsCHLI1 and PsCHLI2) were revealed recently by the whole genome sequencing, but their molecular features are not fully characterized. In this study, PsCHLI1 and PsCHLI2 cDNAs were identified by PCR-based cloning and sequencing. Phylogenetic analysis showed that PsCHLIs were derived from an ancient duplication in legumes. Both PsCHLIs were more highly expressed in leaves than in other organs and downregulated by abscisic acid and heat treatments, while PsCHLI1 was more highly expressed than PsCHLI2. PsCHLI1 and PsCHLI2 encode 422- and 417-amino acid proteins, respectively, which shared 82% amino acid identity and were located in chloroplasts. Plants with a silenced PsCHLI1 closely resembled PsCHLI1 and PsCHLI2 double-silenced plants, as both exhibited yellow leaves with barely detectable Mg-chelatase activity and chlorophyll content. Furthermore, plants with a silenced PsCHLI2 showed no obvious phenotype. In addition, the N-terminal fragment of PsCHLI1 (PsCHLI1N, Val63-Cys191) and the middle fragment of PsCHLI1 (PsCHLI1M, Gly192-Ser336) mediated the formation of homodimers and the interaction with CHLD, respectively, while active PsCHLI1 was only achieved by combining PsCHLI1N, PsCHLI1M, and the C-terminal fragment of PsCHLI1 (Ser337-Ser422). Taken together, PsCHLI1 is the key CHLI subunit, and its peptide fragments are essential for maintaining Mg-chelatase activity, which can be used to improve photosynthetic efficiency by manipulating Mg-chelatase in pea.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Mg-Chelatase CHLI Subunit in Pea (Pisum sativum L.)

Wang

Zhu

et al. 2022

Front. Plant Sci.

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“…Clover yellow vein virus (pCIYVV), pea early browning virus (PEBV), bean pod mottle virus (BPMV), and cucumber mosaic virus (CMV) have all been developed as silencing vectors for legume species (Constantin et al, 2004;Gronlund et al, 2010;Luo et al, 2013Luo et al, , 2016Masuta et al, 2000;Nagamatsu et al, 2007;Zhang & Ghabrial, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Tobacco rattle virus (TRV) has been employed as a VIGS vector in Nicotiana benthamiana , Arabidopsis thaliana , and tomato successfully (Burch‐Smith et al, 2006; Liu et al, 2002, 2004). Clover yellow vein virus (pCIYVV), pea early browning virus (PEBV), bean pod mottle virus (BPMV), and cucumber mosaic virus (CMV) have all been developed as silencing vectors for legume species (Constantin et al, 2004; Gronlund et al, 2010; Luo et al, 2013, 2016; Masuta et al, 2000; Nagamatsu et al, 2007; Zhang & Ghabrial, 2006). However, limited host ranges and phenotypic symptoms associated with the virus itself has restricted the utility of VIGS as a functional genomics tool in this group of plants.…”

Section: Introductionmentioning

confidence: 99%

A fast and efficient method to introduce apple latent spherical virus to legume plants via Agrobacterium rhizogenes‐mediated transformation of hairy roots

et al. 2022

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Virus‐induced gene silencing (VIGS) is a functional genomics tool used to determine the function of unknown genes or assess the impact of gene silencing on plant phenotype. However, VIGS methods for analyzing gene function are not equally efficient across species, and virus inoculation is difficult for some legume species. We describe a fast and efficient inoculation method using Agrobacterium rhizogenes K599 harboring an apple latent spherical virus (ALSV) full‐length cDNA clone. Pisum sativum cv. AAC Lacombe and Lens culinaris cv. CDC Viceroy showed silencing rates of 100% and 47.7%, respectively, within 30 days starting from seed. To our knowledge, this is the first report of virus inoculation by A. rhizogenes‐mediated introduction to legume plants. This work paves the way for high throughput gene function screening in Pisum sativum and other closely related legume crops using ALSV as a symptomless VIGS vector.

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“…However, Fe 3+ , Mg 2+ , Ca 2+ , and Mn 2+ as impurities in WPA are necessary medium trace elements for the growth of plants. It is well‐known that magnesium is an essential element in chlorophyll, iron is an important plant material of aerobic respiration enzymes (it also participates in the formation of chlorophyll), and calcium can promote a variety of metabolic processes in the plant body . On the one hand, the removal of impurities (Fe 3+ , Mg 2+ , Ca 2+ , and Mn 2+ ) is necessary to produce high purity fertilizer; however, on the other hand, these medium trace elements (Fe, Mg, Ca, and Mn elements of chelating state) are added to base high purity fertilizer to produce compound fertilizer .…”

Section: Introductionmentioning

confidence: 99%

Chelation of metal ions with citric acid in the ammoniation process of wet‐process phosphoric acid

Wan

Luo

et al. 2019

Can J Chem Eng

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A new process is proposed in which citric acid is added during the ammoniation process of wet-process phosphoric acid (WPA) to chelate metal ions to produce fertilizer ammonium dihydrogen phosphate (MAP) containing medium trace elements. The parameters of the chelation reaction were studied systematically and the results show that the optimal reaction condition is pH 3.0, reaction time 60 minutes, temperature 50.0 C, and citric acid content 1.48%. The XRD patterns show that the waterinsoluble compounds are complex mixtures, and the composition of the waterinsoluble compounds are changed through the addition of citric acid. FTIR and XPS analyes further demonstrate that water-insoluble compounds are complex mixtures. The XRD patterns of the MAP products indicate that the structure and morphology of the MAP were changed by the addition of citric acid. In addition, the contents of major elements, medium elements, and trace elements in the MAP product are 68.72%, 0.24%, and 0.50%, respectively, which is consistent with the relevant China national standard. K E Y W O R D Sammonium dihydrogen phosphate, chelation, citric acid, wet-process phosphoric acid

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Disturbance of chlorophyll biosynthesis at Mg branch affects the chloroplast ROS homeostasis and Ca2+ signaling in Pisum sativum

Cited by 9 publications

References 34 publications

Molecular Characterization of Mg-Chelatase CHLI Subunit in Pea (Pisum sativum L.)

Molecular Characterization of Mg-Chelatase CHLI Subunit in Pea (Pisum sativum L.)

A fast and efficient method to introduce apple latent spherical virus to legume plants via Agrobacterium rhizogenes‐mediated transformation of hairy roots

Chelation of metal ions with citric acid in the ammoniation process of wet‐process phosphoric acid

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