Disturbance of systemic antioxidant profile in nonsmall cell lung carcinoma

Abstract

The present study aimed to determine the alterations of antioxidant activities in erythrocytes from patients with nonsmall cell lung carcinoma (NSCLC).A comparative study of the systemic antioxidant activities in red blood cell lysate from subjects with NSCLC and healthy control subjects was conducted. The antioxidants catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured using chemical kinetic reactions under spectrophotometry.In total, 189 cases of mostly advanced-stage IIIB or stage IV NSCLC and 202 healthy controls were studied. In subjects with lung cancer, there was similar catalase activity, lower SOD activity (median (interquartile range) 13.4 (9.0-27.2) versus 48. ) compared with controls. The antioxidant activities in lung cancer subjects were not associated with age, sex, smoking status, or tumour cell types. However, more advanced disease (stage IV compared with stage IIIB) was associated with lower SOD activity. Using multivariable analysis, the presence of lung cancer independently predicted SOD and GPx activities.In conclusion, nonsmall cell lung carcinoma in Chinese subjects is associated with alterations in systemic antioxidant activities, which may play an important role in carcinogenesis.

Decreased Selenium-Binding Protein 1 Enhances Glutathione Peroxidase 1 Activity and Downregulates HIF-1α to Promote Hepatocellular Carcinoma Invasiveness

“…Many studies have already reported that GPX1 may protect cancer cells under conditions of severe oxidative stress as it has been observed that increased GPX1 activity can inhibit apoptosis (14,15), reduce tumor sensitivity toward ROSgenerating anticancer drugs (17,18), and promote the more malignant stages of cancer (16). Our findings showed that SBP1 could greatly inhibit the activity, but not expression, of GPX1 in cancer cells both in vitro and in vivo; the translocation of GPX1 to the nucleus in cancer cells under oxidative stress may facilitate the antioxidant functions of GPX1, whereas the formation and combination of GPX1 and SBP1 nuclear bodies might inhibit this process.…”

“…GPX1 is a ubiquitously expressed antioxidant enzyme that scavenges organic hydroperoxides and protects cells from reactive oxygen species (ROS) and hydrogen peroxide-induced or -dependent apoptotic injury (14,15). Elevated GPX1 activity was reported to protect cancer cells from oxidative stress and anticancer agents (16)(17)(18). Previous studies using coimmunoprecipitation showed that these 2 distinct selenium-containing proteins (GPX1 and SBP1) can form a physical association that facilitates their interactions (19), but their possible roles in cancer development are still unknown.…”

Antioxidant enzymes and cancer

“…The serum levels of SOD, GPx and CAT and vitamin C and E were significantly declined in patients with multiple myeloma whereas malondialdehyde levels were elevated as compared with controls studied recently [45] . A comparative study of the systemic antioxidant activities in red blood cell lysate from subjects with non-small cell lung carcinoma (NSCLC) and healthy control subjects was conducted [46] . In this study, in subjects with lung cancer, there was similar CAT activity, lower SOD activity and higher GPx activity compared with controls.…”

EXPERIMENTAL CARDIOVASCULAR AND LUNG RESEARCH Evaluation of the impact of radical tumor resection in lung cancer patients on the activity of selected antioxidant enzymes

“…These antioxidants can intercept, scavenge, and neutralize radicals and their reactive intermediates. The most significant antioxidant enzymes are superoxide dismutase (SOD), occurring in 3 isoforms, catalase (CAT), and glutathione peroxidase (GPX) [12, 13]. …”

“…Antioxidants are mainly situated intracellularly [13]. The term “oxidative stress” is defined as the adverse condition resulting from an imbalance between cellular oxidants (mostly ROS and RNS) and antioxidants (enzymatic, such as CAT and SOD, and others).…”